Uji Kandungan Protein Kasar pada Pakan Ternak

Summary

This video demonstrates the process of testing crude protein, fat, ash, and water content in animal feed at the Faculty of Veterinary Medicine, Airlangga University. The procedure involves several steps: grinding and homogenizing the sample, weighing, and wrapping it for the destruction process, followed by adding chemicals like sulfuric acid and hydrogen peroxide. The process then includes distillation and titration using solutions like boric acid, BCG, and methyl red. The video provides a detailed walkthrough, explaining the tools and techniques involved in testing feed quality, aiming to improve the understanding of animal nutrition testing.

Takeaways

• 😀 The video is from the Faculty of Veterinary Medicine at Airlangga University, demonstrating an animal feed testing process.
• 😀 The primary tests conducted are for crude protein, crude fat, ash, and water content in animal feed.
• 😀 Various chemicals are used in the testing process, including HCl (0.2%), boric acid (4%), BCG (0.1%), and methyl red (0.1%).
• 😀 Equipment involved in the process includes Erlenmeyer flasks, glass beakers, measuring cups, distillation flasks, and paper for wrapping samples.
• 😀 The first step is grinding and homogenizing the sample using a blender before moving to the weighing phase.
• 😀 Each sample is weighed precisely, 0.5 grams, to ensure faster destruction and efficient testing.
• 😀 The samples are placed in coded destruction tubes for further processing.
• 😀 Destruction of the sample begins with the addition of sulfuric acid and a jealous tablet, followed by nitrogen destruction.
• 😀 During the destruction process, hydrogen peroxide is added to each sample for further processing, and the mixture turns from black to yellow-green.
• 😀 After destruction, 50 ml of distilled water is added to the samples, followed by 50 ml of sodium hydroxide, and they are homogenized.
• 😀 The distillation process follows, involving the addition of boric acid, BCG, and methyl red to the samples in Erlenmeyer flasks for nitrogen distillation.
• 😀 Prior to distillation, the nitrogen distillation equipment is washed thoroughly to avoid contamination and ensure clean results.
• 😀 The distillation process changes the color of the samples, and the final titration involves turning the green solution to pink.
• 😀 The video concludes with thanks and a hope that the process is useful to the viewers, wishing peace and blessings upon them.

Q & A

What is the main objective of the video?

The video demonstrates the process of testing crude protein, crude fat, ash, and water in animal feed at the Faculty of Veterinary Medicine, Airlangga University.

What chemicals are used in the testing process?

The chemicals used include hydrochloric acid (HCl) at a 0.2% concentration, boric acid at 4%, BCG at 0.1%, and methyl red at 0.1%.

What are the key tools required for the testing process?

The tools required are Erlenmeyer flasks, glass beakers, measuring cups, distillation flasks, destruction tubes, and paper (used for sample wrappers).

What is the first step in preparing the sample for testing?

The first step is to grind the sample using a blender and then homogenize it.

Why is 0.5g of the sample used in the testing process?

0.5g of the sample is used to speed up the destruction process, as a smaller sample size allows for a faster reaction.

What is the purpose of wrapping the sample in paper?

The sample is wrapped in paper to facilitate its placement in the destruction tube and ensure easy handling during the process.

What role does sulfuric acid play in the destruction process?

Sulfuric acid helps break down the sample during the destruction process, assisting in the release of nitrogen for further analysis.

What happens during the destruction process?

During the destruction process, a chemical reaction occurs with the addition of sulfuric acid and hydrogen peroxide, turning the thick black liquid into a clear yellow-green color over 30 minutes.

What is the purpose of the distillation process?

The distillation process is used to separate the components of the sample, allowing for the testing of crude protein by heating and extracting nitrogen.

Why is the nitrogen distillation tool washed before use?

The nitrogen distillation tool is washed to ensure that any residues from previous tests do not affect the accuracy of the current test results.

How is the protein content determined in the test?

The protein content is determined by titrating the distillation solution in tubes A and B, where a color change from green to pink indicates the amount of nitrogen, and thus protein, in the sample.

What happens if tube A and B are different colors after distillation?

If tube A and B are green, it indicates they contain the sample. If the control tube remains unchanged, it shows that it does not contain a sample. The color change during titration helps determine protein levels.