Uji Kadar Kalsium

Summary

In this video, the presenter from the Faculty of Animal Husbandry and Agriculture at Diponegoro University demonstrates the process of testing for calcium mineral content in a sample. The procedure includes the use of strong acid solutions, atom absorption spectrophotometry, and a series of lab tools such as an analytical balance, glassware, and a spectrophotometer. The steps covered in the video involve sample ignition, dilution, and calculation of calcium concentration in the sample. The presentation emphasizes the importance of accurate measurement and careful preparation to obtain precise results.

Takeaways

• 😀 The video is about testing mineral content, specifically calcium, in feed materials at the Faculty of Animal Husbandry and Agriculture, Universitas Diponegoro.
• 😀 The main purpose of the test is to measure the calcium content in a sample using inorganic materials and strong acid solutions.
• 😀 The testing process involves using equipment like atomic absorption spectrophotometry (AAS), a balance, filter paper, measuring flasks, and a glass funnel.
• 😀 The procedure begins with the ignition of the sample in a furnace to remove organic material.
• 😀 After the sample is ignited, it is cooled in a desiccator before further processing.
• 😀 The sample undergoes dilution, and the concentration of calcium is then measured using AAS.
• 😀 The formula to calculate calcium concentration is: C = (A × V × 10000) / (W × D), where C is the concentration, A is the absorbance reading, V is the volume of the sample, W is the sample weight, and D is the dilution factor.
• 😀 The video includes visual cues of music playing during the lab process to indicate transitions between steps.
• 😀 The use of safety equipment and careful handling of reagents and instruments is emphasized in the lab setting.
• 😀 The overall process focuses on precision and accuracy to determine the calcium content in the feed material.

Q & A

What is the objective of the experiment described in the script?

The objective of the experiment is to measure the calcium mineral content in feed samples using atomic absorption spectroscopy (AAS).

What is the principle behind the calcium content measurement in this experiment?

The principle involves dissolving the feed sample with a strong acid solution and then using atomic absorption spectroscopy to measure the calcium concentration, based on the absorption of light by calcium atoms.

What equipment is necessary to perform the calcium content analysis?

The required equipment includes an atomic absorption spectrophotometer (AAS), analytical balance, filter paper, volumetric flasks and pipettes, beakers, funnels, nitric acid (HNO₃), and the feed sample.

How is the sample prepared for the analysis?

The feed sample is dried and placed in a suitable container to prepare it for further processing.

What does the sample destruction process (ashing) involve?

The sample is heated to burn off the organic components, which results in the destruction of the organic material in the sample.

Why is nitric acid used in the experiment?

Nitric acid is used to digest the sample and break down the organic matter, facilitating the release of calcium into a form that can be measured.

How is the sample diluted after digestion?

Once the digestion process is complete, the sample is diluted with distilled water to bring it to a suitable volume for measurement.

How is the calcium concentration measured in the sample?

The diluted sample is placed in the atomic absorption spectrophotometer (AAS), which measures the calcium concentration based on absorbance readings.

What formula is used to calculate the calcium content, and what do the variables represent?

The formula used is: C = (A * P) / W * 10,000, where C is the calcium concentration (ppm), A is the absorbance reading from AAS, P is the dilution factor, and W is the sample weight.

What is the role of the atomic absorption spectrophotometer (AAS) in this experiment?

The AAS is used to measure the absorbance of light by the sample, specifically to quantify the calcium content in the feed sample.