Penentuan Kadar Miconazole dalam salep dengan menggunakan metode Spektrofotometri Uv-Vis

Summary

This transcript outlines a laboratory procedure for determining the concentration of miconazole in cream using UV-Vis spectrophotometry. The process begins with the preparation of standard solutions and weighing miconazole nitrate, followed by sample preparation and dilution. The spectrophotometric analysis includes measuring absorbance at specific wavelengths, ensuring the solution is clear and homogeneous. The script covers all the necessary steps for proper analysis, from sample handling to measurement, providing a comprehensive guide for students or researchers performing this experiment.

Takeaways

• 😀 The purpose of the practical is to analyze the concentration of miconazole in a cream formulation using UV-Vis spectrophotometry.
• 😀 Miconazole nitrate standard and 95% ethanol are the main chemicals used for the experiment.
• 😀 The process begins with weighing the standard miconazole nitrate and preparing a solution using ethanol for dilution.
• 😀 A balance (analytical scale) and glassware are essential tools for measuring and preparing solutions.
• 😀 The procedure includes preparing both the standard solution and the sample solution of miconazole cream in ethanol.
• 😀 After dissolving the miconazole nitrate in ethanol, the solution is transferred to a 100 mL volumetric flask and made up to the mark.
• 😀 The sample of miconazole cream is dissolved similarly, with excess undissolved parts being filtered to ensure clarity for UV-Vis analysis.
• 😀 Both the standard and sample solutions are diluted to a final volume of 25 mL in separate flasks.
• 😀 UV-Vis spectrophotometry is used to measure absorbance, with the blank consisting of the 95% ethanol solution.
• 😀 The absorbance measurements are performed at a wavelength range of 250 nm to 400 nm, with the maximum absorbance wavelength of the standard being identified first.

Q & A

What is the objective of the experiment described in the script?

The objective of the experiment is to analyze a compound using UV-Vis spectrophotometry, specifically to determine the concentration of miconazole in a cream formulation.

Which methods and equipment are used in the experiment?

The experiment uses UV-Vis spectrophotometry as the primary method. The equipment involved includes an analytical balance, glassware, and the UV-Vis spectrophotometer.

How is the standard solution of miconazole prepared?

The standard miconazole solution is prepared by weighing 0.02 grams of miconazole nitrate, dissolving it in 95% ethanol, and transferring it to a 100 mL volumetric flask, ensuring the solution is homogeneous.

What is the procedure for preparing the miconazole sample for testing?

The miconazole sample (1 gram) is weighed, dissolved in 95% ethanol in a beaker, and filtered to ensure a clear solution. Then, it is transferred to a 100 mL volumetric flask and diluted to the mark with ethanol.

Why is the sample solution filtered during preparation?

The sample solution is filtered to remove any undissolved components of the cream, ensuring that only the dissolved miconazole is tested in the spectrophotometer.

What is the purpose of diluting the standard and sample solutions?

The dilution of both the standard and sample solutions is done to ensure the concentration falls within the measurable range of the UV-Vis spectrophotometer, making the analysis accurate.

How is the UV-Vis spectrophotometer set up for the analysis?

The UV-Vis spectrophotometer is set to measure absorbance with the wavelength range set from 250 nm to 400 nm. The samples are placed in cuvettes, which are carefully cleaned before use.

What is the importance of the blank solution in the analysis?

The blank solution, which is 95% ethanol, is used to zero the spectrophotometer before measuring the standard and sample solutions, ensuring that only the absorbance of miconazole is measured.

What happens after the standard solution and sample are placed in the spectrophotometer?

Once the standard and sample solutions are placed in the spectrophotometer, the absorbance at the wavelength corresponding to the maximum absorption of miconazole is measured. This data is used to create a calibration curve.

How is the wavelength for measurement determined?

The wavelength for measurement is determined by identifying the maximum absorption peak in the standard solution, which occurs at 272 nm, and then measuring absorbance at this wavelength for both the standard and the sample.