TEKNIK INKUBASI BAKTERI DENGAN CAWAN PETRI DI LABORATORIUM MIKROBIOLOGI FARMASI DAN BIOLOGI SEL

Summary

In this video, Wahyuna, Indra, and Sungsang take viewers on a tour of a biology department laboratory. They demonstrate the process of bacterial incubation after spreading, explaining the use of a glass rod for spreading and how bacterial cultures are handled. The lab maintains optimal conditions for bacterial and fungal incubation, with temperatures ranging from 36 to 37°C. Special care is taken to prevent condensation from affecting the culture medium. Viewers are encouraged to report any issues with leftover bacteria in the incubator to the supervising staff, ensuring proper lab management.

Takeaways

• 😀 The video begins with a warm welcome to the biology department and the hosts introducing themselves.
• 😀 The hosts take the viewers on a tour of a laboratory in the department, specifically a microbiology and cell biology lab.
• 😀 The primary activity demonstrated in the lab is the process of bacterial incubation following the spreading technique.
• 😀 The spreading technique involves using a tool called a 'Spider', which is a glass rod, to spread bacteria on a culture plate.
• 😀 After spreading the bacteria, the next step is to label the plates with names and then proceed to incubation.
• 😀 The bacterial incubation is carried out at a temperature range between 36°C and 37°C, using a bacterial incubator.
• 😀 The incubator is used for both bacterial and fungal incubation in the lab.
• 😀 There is a specific technique for placing bacterial culture plates in the incubator, which involves ensuring that the plates are placed upright to prevent condensation from dripping onto the medium.
• 😀 Condensation is avoided to prevent contamination of the media with water droplets, which could affect the bacterial growth process.
• 😀 If students find old bacterial cultures in the incubator, they are encouraged to report them to the responsible lecturer or laboratory supervisor.

Q & A

What is the main activity being demonstrated in the lab today?

The main activity being demonstrated is the incubation of bacteria after spreading them on a culture medium.

What is the role of the 'spreader' in the bacterial culturing process?

The spreader, which is a glass rod, is used to evenly spread the bacterial sample across the surface of the culture medium.

How is the bacterial culture incubated after spreading?

After spreading the bacteria, the sample is placed in an incubator, typically at a temperature between 36 to 37 degrees Celsius, to promote bacterial growth.

Why is it important to incubate the bacterial culture at 36-37 degrees Celsius?

This temperature range is optimal for the growth of many bacteria, particularly those that thrive at body temperature.

What is the purpose of placing a petri dish upside down in the incubator?

Placing the petri dish upside down prevents condensation from dripping onto the agar surface, which could interfere with the bacterial growth and experiment.

What could happen if condensation forms inside the petri dish?

If condensation forms, it could cause the droplets to fall onto the culture medium, potentially affecting the bacterial growth or contamination of the sample.

What should you do if you find old bacterial cultures in the incubator?

If old bacterial cultures are found in the incubator, they should be reported to the supervising professor or laboratory staff for proper handling.

What is the significance of labeling the bacterial culture plates with a marker?

Labeling the culture plates with the name and other relevant details ensures proper identification and tracking of the samples, which is essential for record-keeping and analysis.

What is a 'spreader' in microbiology, and how is it used?

A 'spreader' is a glass rod used in microbiology to spread bacterial samples evenly over the surface of an agar plate. It helps to create a uniform bacterial lawn.

What are the potential risks of improper incubation of bacteria?

Improper incubation can lead to inaccurate results, such as overgrowth, contamination, or failure to grow the bacteria as expected, potentially invalidating the experiment.