Pemeriksaan Feses Metode Tidak Langsung (Pengapungan) Untuk Deteksi Telur Cacing

nurlaili farida muhajir
12 Jul 202104:35

Summary

TLDRThis video demonstrates a laboratory procedure for testing fecal samples using an indirect method. The process involves using a saturated NaCl solution to float parasitic eggs in feces. The sample is mixed, filtered, and left to settle. After 45-60 minutes, a microscope slide is prepared for staining with a 1-2% solution to examine the sample for eggs. The careful preparation ensures clear observation under a microscope, providing an essential tool for identifying intestinal parasites in a non-invasive manner.

Takeaways

  • πŸ˜€ The indirect fecal examination method is used to detect worm eggs in feces.
  • πŸ˜€ The principle involves using a saturated NaCl solution, where worm eggs float on the surface of the liquid.
  • πŸ˜€ Step 1: Fill a beaker with approximately 10 ml of saturated NaCl solution.
  • πŸ˜€ Step 2: Add about half a teaspoon of fecal sample into the beaker.
  • πŸ˜€ Step 3: Mix the fecal sample gently until the solution becomes homogeneous.
  • πŸ˜€ Step 4: Prepare a clean glass slide and a test tube for the filtration process.
  • πŸ˜€ Step 5: Pour the mixture into the test tube and filter through two layers of gauze to remove solid elements.
  • πŸ˜€ Step 6: Allow the solution to stand for 45 minutes to 1 hour for proper separation.
  • πŸ˜€ Step 7: After the settling period, prepare a microscope slide for observation.
  • πŸ˜€ Step 8: Use a 1% or 2% iodine solution for staining the specimen on the slide.
  • πŸ˜€ Step 9: Use tweezers to carefully place the specimen on the slide, ensuring no air bubbles are trapped.
  • πŸ˜€ Step 10: The specimen is now ready for examination under a microscope.

Q & A

  • What is the purpose of the indirect feces examination method mentioned in the script?

    -The purpose of the indirect feces examination method is to detect parasitic eggs, specifically worm eggs, which float to the surface of a saturated NaCl solution when mixed with fecal matter.

  • What role does the saturated NaCl solution play in this examination process?

    -The saturated NaCl solution is used because it has a density that allows parasitic eggs, such as those of worms, to float when mixed with feces, aiding in their detection.

  • How long should the feces and NaCl solution mixture be left to stand?

    -The mixture should be left to stand for approximately 45 minutes to an hour to allow the parasitic eggs to float to the surface.

  • What is the first step in preparing the fecal specimen for examination?

    -The first step is to fill a chemical glass with about 10 ml of saturated NaCl solution, then add about half a teaspoon of the fecal specimen.

  • What should be done after adding the fecal specimen to the NaCl solution?

    -After adding the specimen, the mixture should be gently stirred to break up the fecal matter and create a homogeneous solution.

  • Why is filtering done with a two-layer gauze during the process?

    -The filtering with a two-layer gauze is done to remove any solid elements from the mixture, ensuring that only the liquid portion is used for further examination.

  • What is the purpose of using a glass slide and cover glass in this procedure?

    -A glass slide and cover glass are used to prepare the sample for microscopic examination, ensuring that the sample is properly prepared for staining and observation.

  • What staining solution is recommended for the microscopic examination?

    -A 1% or 2% Lugol's iodine solution is recommended for staining the specimen before it is examined under the microscope.

  • What should be checked before examining the prepared sample under a microscope?

    -Before examining the sample, it should be ensured that there are no air bubbles or excess water between the cover glass and the specimen.

  • What should the examiner look for during the microscopic examination?

    -The examiner should look for the presence of parasitic eggs, specifically those of worms, which will appear after proper staining.

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Related Tags
Feces ExaminationLaboratory ProcessParasitologyMedical TestingNaCl SolutionMicroscopyScientific ProcedureEgg DetectionHealth ScreeningParasitic Infections