Alat Elisa

Summary

This video script outlines the process of operating a diagnostic machine for HBsAg testing. It covers the setup, including selecting testing parameters, adjusting settings for washing cycles, and entering program names for tests. The script demonstrates step-by-step instructions on configuring the system, including reagents, wavelengths, and calibration. It highlights the process of setting up samples, starting tests, and interpreting results. The tutorial emphasizes safety and proper procedures, such as labeling and printing results. It concludes with a reminder to clean up and turn off the equipment after use.

Takeaways

• 😀 The equipment can be set up either vertically or horizontally, with options available for both configurations.
• 😀 The device includes settings for cleaning cycles, with options to adjust the number of washes and the volume of cleaning fluid.
• 😀 The equipment can be customized to wash specific numbers of strips, with settings to wash anywhere from a few to a full plate depending on the number of samples.
• 😀 The washing cycle can be configured for multiple passes (e.g., 6 times), and the user can adjust the cycle's intensity and duration.
• 😀 When setting up the equipment, it's important to input the correct reagent information, especially when the system is open and can use a variety of reagents.
• 😀 A blank reading is performed to calibrate the system, ensuring accurate measurements of absorbance.
• 😀 The program configuration involves selecting a test, entering sample information (e.g., sample name), and specifying test parameters like absorbance and wavelength.
• 😀 For specific tests, a sample tray is used, with samples loaded according to their code (e.g., A1, B1) to ensure accurate tracking and processing.
• 😀 The equipment includes options to adjust the testing speed (e.g., slow or fast) depending on the required test parameters.
• 😀 After completing the test, results can be printed or saved in the system's memory, with options to view or export the report later.
• 😀 Proper waste disposal protocols must be followed after testing, and the machine must be turned off to ensure safety and compliance with lab standards.

Q & A

What is the initial setup step for the device after turning it on?

After turning on the device, the test plate is placed in the machine, and the settings for the number of wash cycles and wash buffer volume need to be configured.

Can the device be placed in different orientations?

Yes, the device can be placed either vertically or horizontally, depending on the available space and configuration needs.

How do you determine the number of wash cycles for the test?

The number of wash cycles can be adjusted in the settings, with options typically ranging from 1 to 6 cycles based on the test requirements.

What is the difference between using a strip and a full plate for testing?

A strip is used when only a few sample wells are needed, while a full plate is used when all wells need to be tested. The choice depends on the number of samples being processed.

What should you do after selecting the appropriate wash cycle and sample type?

After selecting the wash cycle and sample type, proceed by configuring the test parameters, such as wavelength, absorbance levels, and reagent settings.

Is it necessary to use proprietary reagents with this device?

No, the device is an open system, meaning it can accept non-proprietary reagents as well as those provided by the manufacturer.

What is the purpose of setting the absorbance and cutoff values during configuration?

Setting the absorbance and cutoff values helps to define the boundaries for positive or negative results. The cutoff value is used to distinguish reactive (positive) from non-reactive (negative) results.

What happens after the test parameters are configured?

Once the parameters are configured, the samples are inserted, and the test is started. The device will run the test and display the results once completed.

How can the test results be retrieved after completion?

The test results can be retrieved by either printing them directly from the device (if a printer is connected) or by accessing the results in the device's memory or report section.

What safety measures should be followed after completing a test?

After the test, all infectious waste should be disposed of according to safety protocols, and the device should be turned off and cleaned as necessary.