[Video Praktikum] Ahmad Yudhistira Prasetyo - MBI Amanatul Ummah - Permeabilitas Membran Sel

Summary

In this video, a group of students from Madrasah Bertaraf Internasional, Pacet Mojokerto, conducts an experiment to test the effects of temperature and alcohol on the permeability of turmeric membrane. They use various solutions like 70% alcohol, 35% alcohol, ice water, and room temperature water, observing the color changes in turmeric samples over time. The experiment demonstrates that alcohol impacts the concentration of pigment in turmeric, while temperature affects the membrane's permeability, with lower temperatures strengthening hydrogen bonds in proteins, and higher temperatures weakening them. The results highlight how external factors can influence cellular structures.

Takeaways

• 😀 The experiment investigates the impact of temperature and alcohol on the permeability of turmeric membrane.
• 😀 The study uses various solutions, including 70% alcohol, 35% alcohol, ice, room temperature water, and turmeric rhizomes.
• 😀 The tools and materials used include scissors, white paper, knives, transparent glass containers, stopwatch, rulers, syringes, and towels.
• 😀 The procedure begins with cutting turmeric into 1x1 cm pieces, rinsing them, and drying them with a towel or tissue.
• 😀 The turmeric samples are then immersed in different treatments and observed for 15-60 minutes, noting the color changes every 5 minutes.
• 😀 Stock solutions are prepared by grinding the turmeric samples, filtering the mixture, and diluting the stock with water in three different ratios (1:1, 1:2, 1:3).
• 😀 The stock solution is compared with the immersion treatments to assess pigment concentration and membrane permeability.
• 😀 The experiment explores how external factors, like alcohol and temperature, can affect membrane permeability by disrupting the structure of the membrane.
• 😀 Alcohol affects the hydrophobic bonds in phospholipids, which can form gaps in the membrane and increase the pigment concentration in the solution.
• 😀 Temperature influences the fluidity of the membrane by affecting hydrogen bonds in proteins, with low temperatures strengthening these bonds and high temperatures weakening them.
• 😀 The results indicate that alcohol increases pigment concentration in turmeric, while temperature can slow down the movement of the turmeric's pigments.

Q & A

What is the focus of the experiment described in the video?

The experiment focuses on testing the effects of temperature and alcohol on the permeability of turmeric cell membranes.

What materials are used in the experiment?

The materials include turmeric, 70% and 35% alcohol, ice, water at room temperature, scissors, white paper, knives, transparent glass cups, stopwatch, ruler, syringe, and tissue or towel.

What is the first step in the experimental procedure?

The first step is cutting the turmeric into pieces of 1x1 cm size and then rinsing and drying it with a towel or tissue.

How long are the turmeric samples observed in the experiment?

The turmeric samples are observed for 15 to 60 minutes, and color changes in each medium are noted every five minutes.

What is the purpose of preparing a stock solution in the experiment?

The stock solution is prepared from the ground turmeric sample and serves as a comparison for the treated media to measure the concentration of pigments.

How is the stock solution diluted in the experiment?

The stock solution is diluted by mixing it with water in three different ratios: 1:1, 1:2, and 1:3.

How is the concentration of pigments measured in the experiment?

The concentration of pigments is determined by comparing the turbidity of the soaking media with the stock solution and its dilutions.

What is meant by 'permeability of the membrane' in this context?

Permeability of the membrane refers to the ability of the membrane to transfer substances across it, which in this experiment is influenced by external factors like temperature and alcohol.

How do alcohol and temperature affect membrane permeability?

Alcohol affects the hydrophobic bonds in the phospholipids, creating gaps in the membrane, while temperature affects the hydrogen bonds in proteins, with high temperatures weakening the bonds and potentially denaturing proteins.

What are the key findings from the experiment regarding alcohol and temperature?

The experiment shows that alcohol increases the concentration of pigments in the turmeric samples, while temperature affects the fluidity of the membrane, with lower temperatures strengthening protein bonds and higher temperatures weakening them.