PEMANTAPAN MUTU INTERNAL PADA PEMERIKSAAN SERUM KONTROL PARAMETER GLUKOSA MENGGUNAKAN FOTOMETER

Summary

This video from Stikes Bina Cipta Husada Purwokerto covers the process of internal quality control in clinical chemistry laboratories, focusing specifically on glucose parameters. It explains the importance of internal quality assurance through three stages: pre-analytic, analytic, and post-analytic. The procedure includes serum control testing to ensure accurate results, using tools like a photometer and glucose reagent. The video also demonstrates how to prepare and analyze control serum, interpret test results, and apply quality control measures to ensure reliable data, ultimately leading to precise and valid glucose testing in clinical practice.

Takeaways

• 😀 Internal Quality Control (IQC) is an ongoing process in clinical labs to ensure accurate and reliable test results.
• 😀 IQC involves three stages: pre-analytic, analytic, and post-analytic, each crucial for maintaining testing accuracy.
• 😀 Control serum is used in IQC to monitor the accuracy of glucose test results.
• 😀 The photometer needs to be calibrated and prepared before testing, with essential materials like gloves, micropipettes, and glucose reagents.
• 😀 The preparation of control serum includes pipetting and incubating with glucose reagent, which produces a pink color when ready for analysis.
• 😀 After testing control serum, glucose concentration is compared to reference values (92-120 mg/dL) to verify its acceptability.
• 😀 Data from glucose testing is recorded daily for 25 days to assess consistency and precision, forming the basis for statistical analysis.
• 😀 The Westgard chart is used to track and evaluate control test results, helping identify errors using statistical rules like 12s, 22s, and 13s.
• 😀 Values that fall outside control limits indicate potential errors, requiring further investigation to identify whether they are random or systematic.
• 😀 Successful internal quality control ensures accurate results and contributes to the overall reliability of laboratory testing and patient care.

Q & A

What is the purpose of internal quality control in a clinical chemistry laboratory?

The purpose of internal quality control is to prevent errors and ensure the accuracy, reliability, and validity of laboratory test results by using serum control, thus maintaining consistent and precise analysis throughout the testing process.

What are the three main phases of quality control in a clinical laboratory?

The three main phases of quality control in a clinical laboratory are the pre-analytic phase, analytic phase, and post-analytic phase, all of which aim to ensure the accuracy of test results.

How is the glucose parameter tested during internal quality control?

The glucose parameter is tested by preparing control serum, using specific laboratory instruments, and following steps such as aspirating water, checking reagent blanks, and measuring the absorbance to ensure accurate results.

What equipment and materials are needed for glucose parameter testing in this internal quality control process?

The required equipment and materials include disposable gloves, micropipettes (1000 µl and 10 µl), test tubes, rack for test tubes, tips (1000 µl and 10 µl), aquabides, and glucose reagent.

What is the significance of reagent blank testing in the glucose analysis process?

Reagent blank testing is important to ensure that the reagent does not interfere with the absorbance reading. Since the reagent blank was previously tested and found to have an absorbance of 0.0033, further testing is not necessary.

Why is it important to maintain calibration during the internal quality control process?

Maintaining calibration ensures that the laboratory instruments provide accurate and reliable results. It also ensures the proper functioning of reagents and the correctness of patient sample identification and eligibility for testing.

How are results evaluated in this internal quality control process?

Results are evaluated by comparing the measured glucose concentration with predefined ranges and limits, such as the upper control limit (UCL) and lower control limit (LCL), based on the true value and standard deviations.

What is the purpose of creating a Westgard chart in the quality control process?

A Westgard chart is used to visualize the quality control results over time, allowing for the identification of potential errors or deviations from acceptable ranges, thus facilitating error detection and corrective action if necessary.

What is the significance of the '12s' rule in Westgard's quality control system?

The '12s' rule indicates that if a single data point exceeds the ±2 SD range, it may signify a random error or potential problem with the testing process. In this case, corrective actions should be taken.

What should be done if two data points exceed the ±2 SD limit in the quality control process?

If two data points exceed the ±2 SD limit, it suggests a systematic or random error, and the results may not be valid. Further investigation should be conducted to identify the cause of the error, and corrective measures should be taken.