Biorisiko Bagian 2 : Science SOP, GLP

Summary

This online training focuses on biosafety management, covering three key components: personnel, engineering, and administrative controls. The script explains risk assessment, the importance of identifying biological hazards, and evaluating laboratory activities. It also highlights good laboratory practices (GLP), including proper safety containers, personal protective equipment (PPE), handwashing, and equipment calibration. Further, it covers the safe handling and transport of biological materials with a focus on packaging procedures. The session emphasizes the significance of maintaining a safe working environment in the lab to ensure the protection of both personnel and the integrity of research.

Takeaways

• 😀 Biosafety management involves three key components: science, engineering, and management.
• 😀 Identifying biological hazards is the first step in risk assessment, which includes evaluating potential dangers in lab work like handling blood or plants.
• 😀 Risk assessment also involves evaluating lab activities, determining biosafety levels, and implementing mitigation strategies.
• 😀 Good Laboratory Practice (GLP) includes using safety containers for biological materials to prevent contamination during transport.
• 😀 Proper personal protective equipment (PPE), like lab coats, gloves, and masks, must be selected based on the specific lab work being done.
• 😀 It's essential to use closed shoes and protective sleeves in the lab, not for fashion but for safety to prevent exposure to biological hazards.
• 😀 There are correct methods for donning and doffing gloves to avoid contamination and spreading risks in the lab environment.
• 😀 Masking standards vary, and fitting tests (fit tests) should be performed to ensure masks effectively protect against aerosols.
• 😀 Washing hands before and after lab work is crucial to minimize contamination and protect both personal and public health.
• 😀 Regular cleaning and equipment maintenance, such as calibrating tools and monitoring freezer temperatures, are vital to ensuring consistent, reliable research outcomes.

Q & A

What are the three key components of effective biosafety risk management?

The three key components of effective biosafety risk management are: 1) Science, which involves researchers and workers; 2) Engineering, which covers lab design and equipment like BSC; 3) Management, which involves administrative controls and procedures.

What is the first step in risk assessment for biosafety?

The first step in risk assessment is identifying the biological hazards associated with the work being performed, such as potential risks from blood samples or plants.

Why is it important to evaluate lab activities during biosafety risk assessment?

Evaluating lab activities is important because the risks can vary depending on the specific procedures being conducted, such as working with viruses, PCR experiments, or centrifugation, which require different safety measures.

What does biosafety level determination entail?

Biosafety level determination involves assessing the required safety protocols based on the identified biological hazards and the type of work being performed, ensuring appropriate containment and protection measures are in place.

How does good laboratory practice contribute to biosafety?

Good laboratory practices help reduce contamination risks and ensure safe handling of biological materials, such as using safety containers for transporting samples and following proper disposal protocols for hazardous materials like needles.

What personal protective equipment (PPE) is typically used in laboratories?

Typical PPE includes lab coats, gloves, goggles, and various types of masks, depending on the specific hazards present in the lab. The choice of PPE is based on the type of work being conducted.

Why is it necessary to wear closed-toe shoes in the laboratory?

Closed-toe shoes are necessary to protect the feet from potential biological hazards and accidents in the lab, preventing contamination or injury from sharp objects or spilled materials.

What are the proper procedures for handling gloves in the lab?

When wearing gloves in the lab, it’s essential not to touch surfaces or objects indiscriminately. Gloves should be removed carefully to avoid contamination and ensure that only the outside is discarded properly.

What is the significance of mask fitting tests in a lab environment?

Mask fitting tests ensure that masks provide adequate protection against aerosol hazards. Proper mask fitting is crucial to prevent the inhalation of harmful biological agents in the lab.

Why is it necessary to calibrate laboratory equipment?

Calibrating lab equipment, such as micropipettes, ensures accurate results in experiments. Without regular calibration, equipment may deliver inaccurate measurements, compromising the quality of research.

What is the correct way to package and transport biological materials?

Biological materials should be packaged in three layers: 1) A primary container to hold the material; 2) A secondary container with absorbent material to contain spills; 3) An outer packaging that is durable and resistant to impacts, sometimes including insulation like Styrofoam or dry ice for temperature-sensitive samples.