Labeling and plate terminology.
Summary
TLDRThe video script is an educational tutorial focusing on the terminology used in mycology, particularly for home cultivators. It explains the meanings behind labels such as 'T' for transfer, 'C' for clone, 'F' for filial generation, and 'Ms' for multipore syringe. The presenter clarifies concepts like spore cultivation, subculturing, and the significance of passage numbers. They also touch on the debate over naming and stabilizing strains before the F5 generation, emphasizing the practical aspects of these processes in mushroom cultivation.
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Outlines
π Introduction to Mycology Terminology
The speaker begins by introducing various terms used in mycology, focusing on 'tpcf Ms and WT'. They explain that 'WT' stands for 'wild type' and 'Ms' stands for 'multipore', often referring to a multipore syringe. The speaker emphasizes the importance of understanding these terms, especially within the home mycology community. They also mention the use of 'F' for 'filial generation' and 'P' for 'passage', noting that 'T' stands for 'transfer' or 'subculturing', a common practice in mycology. The speaker acknowledges the complexity of these terms and the variations in their usage, hinting at the need for clarification and further discussion.
π Deep Dive into Transfer and Passage Numbers
The speaker delves deeper into the concepts of 'transfer' and 'passage' numbers, explaining that 'T0' typically signifies the starting point with spores, while 'T1', 'T2', and so on represent subsequent transfers. They clarify that 'passage' implies a more significant growth and division of mycelium before transfer. The speaker also touches on the historical use of these terms, referencing Paul Stamets' work and the distinction between 'passage' and 'transfer' in the broader scientific community. They emphasize the importance of labeling and understanding one's own system for tracking these numbers, regardless of external debates or variations in terminology.
π± Discussing Cloning and Isolates in Mycology
The speaker discusses the terms 'clone' and 'isolate', explaining that 'C' stands for 'clone' and is used to denote a specific piece of mycelium taken from a culture. They mention 'ISO' as a shorthand for 'isolate', which is often used interchangeably with 'clone'. The speaker also introduces the concept of 'F' numbers, which represent 'filial generations', and begins to explain the significance of these numbers in tracking the genetic stability and progression of a culture. They provide examples of how these terms might be used in labeling and discuss the potential for controversy surrounding the use of 'F' numbers, particularly in the context of naming and stabilizing strains.
π Understanding the Significance of F Numbers and Wild Types
The speaker continues to elaborate on 'F' numbers, using the term 'filial' to describe generations of organisms. They explain that 'F0' typically represents the wild or parent generation, and each subsequent 'F' number indicates another generation of growth and cloning. The speaker also introduces 'WT' or 'wild type', a term used to describe organisms isolated directly from the wild. They discuss the concept of 'land race' and 'legacy' strains, suggesting a shift in terminology within the community. The speaker acknowledges the complexity of these topics and the potential for further exploration in future discussions.
π Concluding Thoughts on Mycology Terminology
In the concluding part, the speaker summarizes the key points discussed, including the importance of understanding terms like 'T' for transfer, 'C' for clone, 'F' for filial generations, and 'WT' for wild type. They reiterate the significance of these terms in the context of mycology and the need for clear communication within the community. The speaker also expresses their intention to create more videos to delve deeper into each term and address any questions from the audience. They acknowledge the length of the video and the complexity of the subject matter, promising to break down future discussions for better clarity and understanding.
Mindmap
Keywords
π‘Multipore Syringe
π‘Wild Type (WT)
π‘Filial Generation (F)
π‘Transfer (T)
π‘Clone (C)
π‘Subculturing
π‘Monocaron
π‘Isolate
π‘Meiosis
π‘Spawn
Highlights
Introduction to the concept of 'multipore syringe' and its abbreviation 'Ms'.
Explanation of 'wild type' and its abbreviation 'WT' in the context of biological samples.
Clarification of the term 'filial' and its abbreviation 'F' in the context of generational lineage.
Discussion on the term 'passage' and its abbreviation 'P', with a note on its alternative use in different communities.
Definition and use of 'transfer' in the process of subculturing, with the abbreviation 'T'.
Explanation of 'T0' as the starting point with spores in the culturing process.
Description of 'T1' as the first transfer or subculture from a spore plate.
Differentiation between 'transfer' and 'passage' numbers in culturing practices.
Importance of understanding one's own labeling system for culturing purposes.
Introduction to the term 'clone' and its abbreviation 'C' in the context of genetic replication.
Explanation of the term 'isolate' and its abbreviation 'ISO', often used interchangeably with 'clone'.
Discussion on the term 'monoculture' and its abbreviation 'M', with a note on its different use cases.
Clarification of 'F' numbers in relation to filial generations and their significance in stabilization.
Practical approach to raising F numbers through spore germination and meiosis.
Controversy and practical considerations regarding the naming and stabilization of strains at different F levels.
Advice on labeling and the importance of personal understanding of the culturing process over standardized terms.
Reflection on the necessity of creating detailed videos for each term due to the complexity of the subject.
Final thoughts on the use of terms like 'multipore land race' and 'legacy' in the context of biological samples.
Transcripts
hello everybody I wanted to go through
some terms today uh looks like the
things the recording uh I wrote all
these up here I don't know I was kind of
hesitant maybe I do need a bigger a
bigger whiteboard uh but this is
tpcf Ms and WT uh before I forget WT
stands for wild type Ms stands for
multipore often there's a multipore
syringe like an S um and there can be
various other letters in this a BRI
viation but MSS or Ms that stands for
multipore I'll come back to that here in
a second but for right now I want to
start at the top so F stands for filial
Generation Um that's F let's see I
probably should have wrote some of this
stuff down here might have to erase some
of this stuff so I'm going to write it
now before I forget um for clarity P
stands for passage and I'm going to get
to that in a second so I won't write it
t stands for transfer okay and there's a
bunch of other words that I'm going to
throw in so transfer or
subculturing uh is what we often
use when we're making essentially when
we take an agar plate uh and we transfer
a piece to a new plate so it's just a
generic word the rest of the world uses
the term subculture say so if you grow
Mamon uh tissue culture like in in agar
plates usually in liquid media we we
usually refer to that subculturing but
for some reason transfer hence the T has
has kind of caught on in the mological
IC community at least the home mological
Community people have all sorts of other
slang words but t on plates if you see a
t like a T1 T2 T3 um or a t0 so let me
let me just start there so t let me
switch so what does t0o mean I'm going
to have to erase a lot of this stuff you
guys my uh aggar or my aggar my uh
whiteboard just basically isn't uh big
enough to write all this stuff and I
don't even I don't even know if I need
to go bigger so I'm going to write these
things and then I'll probably go back
erase them also helps to sort of clear
my thoughts to move on the next topic so
t0 t0 means essentially you are starting
off with spores okay so when you have a
Spore print or even maybe a multi-pore
syringe uh you shoot it under an agar
plate and you label it t0 so the t0 it
might have been streaking it might have
been a grab and drag so t0 without
getting way way way in depth here
because all of these terms are loaded
with uh kind of special cases and and
uses and and different people use them
in slightly different ways some times t0
is essentially spores so you put spores
on an Agger plate okay so what is T1 so
T1 when you take um a transfer from that
plate of spores or anything else let's
say somebody sends you a a a die carry
on on a plate you could if you wanted to
call that t0 like so that would be like
your master culture or whatever again I
don't really like that that term Master
culture not only do have negative kind
of connotations historically but it also
I it just sounds kind of like very very
like in the box like this is the only
culture I ever use and that's often not
the case uh when you start getting into
into this you'll you'll realize that
there is no such thing as like you know
a master like blue Vuitton or a master
GT they just don't exist so for various
reasons which could take hours to
explain um but T1 means essentially
you've taken your aggar plate and you
cut off a little piece you know let's
say that's your t0 so you take your
little square your Ager Punch or
whatever you want to use and you put it
onto a new plate
mhm so that would be your T1 simple as
that so you do that again then you got
your T2 Etc sorry guys I'm writing again
at a kind of an awkward angle here so if
my my uh my my so um subculturing so we
use in the in the rest of the world they
talk about passage numbers so here's
where things get a little bit tricky
because passages H I believe in Paul
stam's old old books you know the the
mushroom cultivator the TMC and uh what
is it was H growing Gourmet and
medicinal mushrooms the ggmm not the mmm
mmgg which is magic mushroom Growers
guide so the Paul stamitz book um the
the growing Gourmet and M and medicinal
mushrooms uh he uses the term passage so
it's it's kind of a little bit
unfortunately it's kind of a slightly
different concept uh than than transfer
transfer is literally it just means a
transfer um so let's let's say if your
your plate here let's just say you had
your mycelium you know and you take you
can take a transfer at any time you know
your uh your little mycelial uh spot
here might only be you know maybe a
centimeter half an inch wide so that
would be appropriate to say you know
you're transferring it and go from t0 to
T1 Etc passage is a little bit different
because passage kind of implies that
you've let this melium grow to the edge
of the plate so you can do some math and
figure out how many mitotic divisions
have occurred so that's where kind of
Passage it's like you're passaging the
edge of this so and then so it gets a
little bit more complicated uh if you
take your transfer from here or you take
your transfer from here uh you know and
you make a new plate this is kind of a
different scenario because that melium
right there is older it's obviously gone
through more mitotic division so then we
get back into the idea of sidence I
don't even want to get that's a whole
other video in it of itself um so Paul
Stam it sometimes it's p number like
Paul um it actually refers to passage um
it's it's an old term that people use
you know just like you're crossing the
Atlantic or whatever you know from
Europe to America it's a passage um so
again that gets into syence and all
kinds of stuff that's not the topic of
this video because otherwise I'm trying
to make this about 15 minutes and it'll
uh it'll turn into three hours very very
shortly if I if I'm not
careful so let's move on so let's just
forget about that one so we got that one
done okay there you go done well that's
really enough again I could have made
whole videos about each one of these
things I just want to know um I just
want to you know when you see a plate
that says you know
t0 you know Ms I always label mine Ms
too because my multipore I know it's t0
but I put Ms just so I don't forget and
you might see something like uh well
this would probably you wouldn't put an
F number we'll get down to that in a
second here um that's a little bit more
complicated but let's let's just stick
with the basic um for right now so let's
add another thing here let's say you put
uh you got a C on there so you've got t
w okay this is look this let's not make
things um a little bit more complicated
than you need to be um you know the most
important thing in all of this you guys
is that you understand what it means on
your plates uh unless you're selling
plates or trading plates it's not really
necessary for other people to be honest
to understand what your your system is
as long as you understand it remember
this is for your inh house use um so
however you want to do this it's up to
you really people get in really really
heated debates you know on online about
like oh you know especially the F number
that's like a whole whole whole like
controversy there um I may get into that
a little bit later I'll probably try to
stay away from it so let's say you got a
uh let's say you have a plate disbled T3
okay that means transfer three so that
means you know wherever you got it from
maybe you got a plate and you've
transferred it so you got a you could
call it a t0 plate your bu gave you that
it's T1 T2 T3 or he might have had a
label on it already or she right maybe
they gave you a plate where that was a
label T2 and you subcultured it or you
you transferred it so you let make it
T3 um so it would be let's say T3 and it
has says something like F4
C1 okay so obviously let's just let's
just use blue Vuitton for since I that's
the only one that's popping in my head
right now and a date uh today I believe
is the 9th so it's May 9th I use
um I I use the the the kind of rest of
the world use it goes date month year so
this would be May May 9th 2024 what the
hell does all this mean okay so again I
made I might could have started with
this but let's say you got this covered
T's already um f we'll get to in a
second so the C1 what does that mean
well C stands for
clone okay so I didn't write it should
be fairly obvious right uh just like in
the Cannabis world you know you cut off
top of a apical marem or or whatever
you're doing you know it could even come
from a tissue culture that's a whole
other thing I want to get into later you
know you can tissue culture and grow uh
tissue explants from Cannabis and all
kinds of other plants in the same way we
do fungi so once you learn how to do
this in fungi it's equally applicable to
mamalian cell culture as well as um you
know plant culture plant tissue culture
so that kind of is another broad term I
don't know what people like to call it
but you may hear the term like tissue
culture or t C this stands for
tissue
culture okay so if you hear that word TC
or if you see it in the literature um
that's what it refers to so we love
acronyms man I I hate you know I kind of
hate it but they're they're just kind of
a necessity because if I keep saying
tissue culture you know to somebody who
already knows I mean you know TC is
tissue culture they're going to get kind
of like frustrated be like why don't you
just say TC or if I keep saying transfer
three the third transfer you know it's
kind of hard to write it on plate too so
that's part of the I mean it's just
convenient right we need to we only only
not sew you know maybe 3 in or 90 mm or
whatever to write everything uh on a
plate and you know maybe your Sharpie is
a little bit duller than it should be
and you know you start writing this
stuff and you're like who uh some people
again I should go back to that some
people like um you know some people like
writing on the edge of the plate I don't
suggest that you guys I would put it
right up on the top so as an example
here now no I got a a Dion from somebody
and I grew that out um and so I just
labeled that arbitrarily I oh I hope
that focusing things um I I was so
instead of writing C1 I wrote actually
F1 clone I don't know I was feeling
verbose that day um so there's like six
little pieces of of tissue there I don't
know how well you can see that but
they're they're growing out nicely and
so later on so I could call this you
know nuk F1 C1 t i could Pro I would
probably call it T1 um and then maybe
the next one I call T2 you can call it
t0 if you want I don't know it's really
up to you um but anyways uh I was I know
exactly what this is again that's that's
kind of a a good point is that like if
you I only have one nanuk culture or
nanuk um I think this is from Dave I
only have one of these so I don't have
to get all like and I know it's from
Dave he gave it a name I'm gonna I'm
going to call it in a Nook like so I
don't need to really worry about F
numbers and all that later but you
know my next plate when I get spores
I'll get back to that so that when I get
spores uh when I fruited it and get
spores from this this will become an F2
and then I'll drop the drop the Clone
part but before we okay I should
probably I just saw another one of my
plates you might see M okay so
m equals
monoculture uh monoc Caron oops so M is
for monoc Caron I just saw that on one
of my plates over there and I don't want
to get into that again in this video
kind of did that on the last videos but
if you see like an
M1 um or M2 or whatever that refers to
Mono Caron but I don't really want to
get into that today that's a whole
different
topic um so clone so c means clone so
you could have C2 C3 let's say you ran a
multiport tub and you know you might get
six or seven phenos another word I don't
want to get in but phenotype and
genotype that's another whole video um
so so that that P or whatever you know
that that's for passage we don't really
use that so let me just get rid of that
right now we don't really use passage
numbers unless you're Paul stamitz or
one of the people that works for funai
perfectti we don't really use P numbers
um so we just stick with
T's um there's some historical reason
for that but again I don't need to get
into that so we' we've covered C clone
so what about these other terms ISO ISO
is short for isolate so an isolate or a
clone I these kind of terms usually get
kind of used
interchangeably and then for people
especially who are first starting out
the The Strain you might say BV uh
strain has a more specific meaning
strain in in most of the rest of the
biological world is synonymous with
isolate so let's you have a let's say
you have a strain of ecoli that's
pathogenic um now maybe a strain might
be in multiple places so you know if
somebody gets uh well you get sick from
the from from the salad bar and somebody
else gets sick from sick from The Burger
Joint somebody else gets sick from The
Shake Shop you might say that's you know
the strain of eoli what what what is it
I forgot the name of that 189 or
whatever it was um whereas in in the
mushroom world at least an ISO generally
means an isolate so that's kind of
synonymous with clone people will often
sometimes mix these things and it's it's
kind of you got to guess kind of how
they're using it uh I that's why I
adopted the term cultigen so in the
mushroom World strain used to be
something like a B+ a GT a blue Vuitton
on that terminology is kind of faded a
little bit because strain normally
refers to a a particular isolate of a
particular
cultigen and then again see how this is
already getting very very very messy so
I would say the cultigen for this is
blue Vuitton it's the second clone that
I took from a maybe a multiport tub so
I've grown fruit and I've Clon those
fruits and obviously I would have a C1
and then this is my C2 and my C3 I don't
know unless you want to start numbering
back CS or whatever and you call it
anything you want uh and the F4 that
would be the generation so let me get to
that next so what the hell so I'm just
going to erase that you guys so I don't
I keep backtracking and I want to say
more about these things but let me just
uh erase these so I don't I don't like
divert my attention here so what the
hell is an F number F stands for filial
filial it means literally it means
brothers and sisters or siblings so let
me just write siblings so we don't got
to all into that gender
let me say just
siblings um so what that means it's just
literally it's a is an old uh term that
means siblings I think it's Greek or
Latin or something so F4 um F4 means
essentially it's gone through four
cycles of kind of uh meiosis so what
happens say your mom and dad you get
together you have a bunch of brothers
and sisters you would call those your
siblings now mostly in the in the plant
world and the animal world we talk about
things like F0 so F0 typically equals
your parents okay and I've noticed this
this is where I know some people are
going to get like really excited about
this the parents are usually referred to
as the
fzero okay so the parents or things that
were wild okay so let's go so say you go
out in the I don't know in some field in
Texas and you pick up a cube or whatever
um you come come back and you clone it
right so you could call that your F0 and
you would that would imply to me that
that was something that you got from the
wild so you could have a plate that
would say like F0 C1 C2 C3 multiple
clones from from fruit uh and then you
could call that your t0 or your T1 if
you wanted to I I tend to like put one
like T1 because usually you're starting
from some sort of wild Source um so I
like I like the transfer I usually start
at T1 but if you want to start it t z
you can uh and again if you want to do
any if there's going to be a lot of
people that will disagree with this I
don't really care um they shouldn't care
because whatever you understand as your
system uh is the right way this is one
of those where there's like there's
really no right way uh and anybody who
tells you there is is full of
right um so I'm just telling you the the
general way and the the kind of most
accepted kind of standard way that we do
this stuff at least in the home myology
community so when you get to so how do
you get so a lot of people say like well
you need to stabilize something right
like how you going to stabilize right
you need to stabilize you need to get it
to you know your F5 or whatever to
stabilize it you know F5 F6 you can't
give it a name because it's not
stabilized that's you guys
because if you see certain very very
famous culturs floating around uh now
they're at f8 F9 F10 and they're still
not stable you guys so um if you're on
an F1 and F2 you want to give it a name
people are going to give you for it
but you know that's very very common in
the mushroom Community we're kind of
used to it after a while um so what the
hell is F5 mean it means filial
Generations so this is where a little
bit of the controversy comes into
because let's say you get a plate uh
let's say it's let say this is
F2 you want to get it to F3 or F4 F5 how
the hell do you do that well the
essential thing here is spores you
guys spores and
meiosis okay so here's the basic idea if
I start off with something let's just
say it's an F2 so I get a plate it's F2
I make some spawn I grow that out and I
get spores okay when I germinate those
spores I can now say that that's F3 this
is where it gets a little tricky because
typically F refers to um the F2 or F3 it
refers to the whole organism and in
animals and plants that means a diploid
our funi never form a diploid if you
remember we always have like n plus n so
we don't get a diploid organism until we
make that basidium or that bidia plural
uh on the mushroom fruit body so
technically our mushrooms um they're not
technically we can't we shouldn't
actually raise the F number but for a
practical purposes you got to assume
that if you got an F2 Generation you
make spores they've undergone meiosis
the subsequent spores the subsequent
fruit the subsequent cultures the
subsequent um whatever uh can be labeled
F3 it's just a practical thing so often
time when I get spores I'll double label
them so if I take a Spore print I might
say F2 spores for me I know that when I
germinate those they're going to become
the F3 okay so when I take those spores
from the F2 and I put them on a new
plate and I germinate them I write F3
okay that's under the assumption that
you're going to take those for you're
going to grow them out you're going to
make more fruit and then when those
fruit undergo meiosis in the bidia
they're going to make the F4 so at some
point you need to Callum F3 wherever you
want to do that is up to you I generally
prer to um if I had F2 spores if I
germinate them I'm going to label those
plates F3 I'm going to label the the
spawn and the tub F3 and when I make my
new spores I'm going to make that F4 so
this is why a lot of the I hate to get
kind of controversial but this is is why
a lot of the old school um people that
don't want you to do they don't want you
to catch up okay so let's say your
typical mushroom generation goes so
that's another word you might see G I'm
not going to go there uh G for
Generation you'll see that in the
literature um we're not going to use
that term here because it's not really
generally used you may see it in Paul
stam's books uh and some of the older
literature um these days we kind of
because G is a is a a whole other G
usually refers to um spawn so when we do
G2 G grain to grain the G is actually a
reference to Generation Um so if you
have a master spawn jar and you turn
that into 10 jars and then you take one
of those 10 and you expand it again and
you do a G2 g g is grain grain to grain
um the G if you see it on like a plate
or a label it means generation so again
if I had a mass Master jar of Spawn and
I did g2g anime 10 jars that would be
you know the the G2 second generation
again if you want to call maybe you're
calling the master and then you got G1
or G2 however you label it you need to
have subsequent numbers you know
ascending numbers so so you get the idea
there again that this is already getting
way more complicated than I thought it
was going to
be you can write questions down at the
bottom uh I might have to do all of
these in a more um like a own their own
little video and I'm probably up to
about yep 20 minutes already oh my
goodness so you guys um I'm going to
like maybe let me just go I might have
to make other videos you guys these
videos are getting way too long and I
just saw it's at 20 minutes already as
you can tell I can I can talk about
these topics forever and ever and ever
so let's just review real quick here
remember we had t for
transfer uh we had C for clone and we
have f for filial um Ms stands for
multipore I talked about that a little
bit already terms WT WT means wild type
okay so this is again something that
doesn't get used in the uh mological
Community but I think should because
it's a great term um you will see it in
the literature talking about bacteria if
you isolate something from the wild you
call it wild type or WT you little
graphs and stuff where they compare like
say A you know a wild Cube to you know I
don't know Enigma or something like that
um you might see
WT land race you know all the plant
people know that are there Cube Land
races anymore probably not um we have
moved cattle and other ruminants all
around the world and I don't really
think this term land race really applies
to cubes anymore or cannabis for that
matter I like the term Legacy I'm going
to try to start using that term a little
bit more um so something like you know
you would say like oh manami you know
from Japan is a a legacy race something
like Burma or Cambodia or you know a
Koso or something like that um you would
say that that was maybe a legacy St rain
or a legacy cultigen H my little bugs I
never see bugs until I
start I don't know what that one is I
had the door open earlier in my
kitchen started pouring down rain the
first big rain we've had this year so
the bugs are coming out um any who back
multipore land race Legacy um whatever
you know whatever word you want to use
and finally mono carryon and I think I'm
going to end it there you guys
because uh I I just realized I'm going
to have to go into these terms maybe in
way way way more detail but I hope that
starts you guys off um somewhere um the
other thing is going to take like an
hour and a half to upload this to
YouTube it's not like the lives where
they go directly I actually have to um
like upload this to YouTube ah the
resolution is much better obviously and
I hope it hasn't been doing that Focus
thing you guys I know I've been moving
around a lot um anyway if you have
questions put them down uh below and uh
I'll be sure I've got a list I don't
know if you guys I got my other
whiteboard um I've got this this is the
list of
videos that I'm going to make
so there's going to be a lot of videos
like six months ago I was like what what
can I possibly make another video about
um yeah I've got 16 there and another
like 15 pages of ideas on my computer so
I hope you guys like that I hope I
didn't miss anything too glaringly
obvious I probably will uh will notice
that I did when I when I rewatch this um
but any who you guys I hope you all have
a good day and um I will we'll talk to
you later for sure bye-bye
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How to say German dates correctly | Ordinalzahlen | German ordinal numbers
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