Praktikum Pemeriksaan Arsenik

Summary

This video demonstrates the process of testing arsenic levels in food, specifically rice, using an arsenic test kit. The procedure involves grinding the rice sample, measuring and mixing with reagents, and monitoring the temperature. After several steps of adding reagents and homogenizing the mixture, a test strip is used to detect the presence of arsenic. The strip is compared to a color chart to determine the arsenic concentration, with a result of 0 ppb indicating no arsenic in the rice sample.

Takeaways

• 😀 The arsenic test requires specific equipment such as a mortar, balance, beaker glass, reaction bottle, thermometer, spatula, and measuring spoons.
• 😀 The sample for the test is 10 grams of rice, which is first ground before weighing.
• 😀 After grinding, the 10-gram rice sample is placed in a beaker glass and dissolved with 100 ml of distilled water (aquadest).
• 😀 The sample is then homogenized using a spatula before beginning the testing process.
• 😀 The temperature of the sample is checked, which in this case is 28°C, ensuring that it is suitable for testing.
• 😀 The sample is transferred into a reaction bottle, and reagents are added to facilitate the testing process.
• 😀 The first arsenic reagent (Reagent 1) is added using a measuring spoon (3 spoons) and the reaction bottle is shaken for 15 seconds.
• 😀 After the first shaking, the reaction bottle is opened, and Reagent 2 is added (2-3 spoons). The bottle is shaken again for 15 seconds.
• 😀 The reaction mixture is allowed to sit for 2 minutes, after which Reagent 3 is added, followed by another 5-second shake.
• 😀 A test strip (T-streak) is inserted into the reaction bottle, and the color is compared against a color chart after waiting for 10 minutes.
• 😀 The final result shows that the rice sample does not contain arsenic pentoxide, as the test strip remains white, indicating a result of 0 parts per billion (ppb).

Q & A

What is the primary purpose of the arsenic test kit in this procedure?

The arsenic test kit is used to detect the presence of arsenic in food and beverages.

What are the key materials required for the arsenic testing procedure?

The key materials include a mortar, balance, beaker glass, reaction bottle, thermometer, spatula, measuring spoons, arsenic color chart, and various reagents.

What is the first step in the procedure for arsenic testing?

The first step is to grind the rice sample into a fine powder using a mortar.

How much rice sample is used for the arsenic test?

A 10-gram rice sample is used for the arsenic test.

What is the role of aquadest water in the arsenic testing process?

Aquadest (distilled water) is used to dissolve the rice sample in the beaker glass, with a total volume of 100 mL.

Why is it necessary to homogenize the sample after adding aquadest?

Homogenizing the sample ensures that the mixture is even and properly prepared for arsenic testing.

What temperature should the sample be at before starting the arsenic test?

The sample temperature should be 28°C before starting the test.

What is the purpose of adding the arsenic reagents to the reaction bottle?

The arsenic reagents are added to the reaction bottle to react with the rice sample, enabling the detection of arsenic.

How long should the reaction bottle be homogenized after each reagent is added?

The reaction bottle should be homogenized for 15 seconds after each reagent is added.

What is the significance of using the T-test strip in the arsenic testing process?

The T-test strip is used to visually determine the arsenic concentration in the sample by comparing the color change on the strip with a color chart.

What does a white color on the test strip indicate about the rice sample?

A white color on the test strip indicates that the rice sample does not contain arsenic pentoxide, with a result of 0 ppb.