Praktikum Patologi Klinik - Pemeriksaan Sel Trombosit dengan Kamar Hitung Improved Neubauer

Summary

This video tutorial demonstrates the process of platelet count analysis using an improved hemocytometer method. It covers the preparation of blood samples, mixing with diluents, and transferring the mixture into a counting chamber. The procedure involves examining the sample under a microscope, focusing on platelet cells, and counting them in four specific areas of the chamber. The final platelet count is calculated using a formula based on the total number of platelets counted. The session concludes with a prayer of thanks.

Takeaways

• 😀 The procedure begins with a greeting and invocation: 'Assalamualaikum, warahmatullahi wabarakatuh'.
• 😀 The laboratory equipment used for the platelet count includes a Thoma pipette, improved chamber, and a microscope.
• 😀 The blood sample is first homogenized before being drawn into the Thoma pipette.
• 😀 The Thoma pipette is filled until the marked line, and a dilution of blood with a diluent solution is prepared.
• 😀 After mixing the blood and diluent in the Thoma pipette, 4-5 drops are discarded to ensure proper mixture.
• 😀 The mixture is then transferred into the counting chamber, ensuring the entire counting area is filled.
• 😀 The sample is left for a moment to allow blood cells to settle at the bottom of the chamber.
• 😀 Under a microscope with a 45x objective lens, platelet cells (thrombocytes) are observed in the designated counting boxes.
• 😀 The platelet count is conducted by counting the number of thrombocytes in four specific boxes.
• 😀 The total platelet count is calculated by multiplying the number of thrombocytes counted in the four boxes by a factor of 500.
• 😀 The process concludes with the statement 'Alhamdulillahirobbilalamin', thanking for the completion of the examination.

Q & A

What is the purpose of this procedure?

The purpose of this procedure is to count the number of platelets in a blood sample using a Thoma pipette and a hemocytometer, ensuring accurate results for medical or laboratory purposes.

What equipment is required for the platelet count procedure?

The required equipment includes a Thoma pipette, hemocytometer, a blood sample, and a dilution solution.

How should the blood sample be prepared before starting the procedure?

The blood sample must be homogenized before beginning the procedure to ensure even distribution of cells.

How is the blood sample transferred to the hemocytometer?

The blood sample, after being mixed with the dilution solution, is transferred into the counting chamber of the hemocytometer until it fills the chamber evenly.

Why is it important to allow the blood cells to settle in the hemocytometer?

Allowing the blood cells to settle ensures that the platelets are evenly distributed at the bottom of the counting chamber, making it easier to count them under a microscope.

What magnification is used to examine the hemocytometer?

The hemocytometer is examined under a microscope using a 45x objective lens to view the platelets clearly.

How are the platelets counted in the hemocytometer?

Platelets are counted within four specific squares of the hemocytometer. The total number of platelets counted in these squares is then used to calculate the platelet concentration.

What formula is used to calculate the platelet count?

The platelet count is calculated using the formula: Platelet Count = 500 × (Total Platelets Counted in Four Squares) ÷ Area (mm³).

What unit is the platelet count expressed in?

The platelet count is expressed in platelets per cubic millimeter (mm³).

What is the significance of the final platelet count result?

The final platelet count provides important information about the platelet concentration in the blood sample, which is crucial for diagnosing various medical conditions related to blood clotting and platelet levels.