AGREGASI TROMBOSIT

Summary

This tutorial provides a detailed guide on performing a platelet aggregation test, a diagnostic procedure that evaluates the ability of platelets to form aggregates. The process includes preparing blood smears with and without additives (such as ADB), observing the slides under a microscope, and calculating the percentage of aggregated platelets. The test results help identify platelet function abnormalities. The video also covers the step-by-step method of staining, observation, and result interpretation, with normal aggregation values ranging from 50-70%. The tutorial aims to help medical professionals understand and conduct this essential laboratory test.

Takeaways

• 😀 The topic of the script is platelet aggregation testing, aiming to assess platelet ability to form aggregates.
• 😀 The procedure involves using citrated blood mixed with specific agents to trigger platelet aggregation.
• 😀 The platelet aggregation test is done by creating blood smears after three minutes of adding the agent and then examining them microscopically.
• 😀 Equipment needed includes agent types like ADB, glass slides, and a microscope for detailed observation.
• 😀 A control smear is prepared first, with no additives to assess the baseline aggregation at zero minutes.
• 😀 A second smear is prepared after adding ADB to the blood and waiting three minutes for aggregation to occur.
• 😀 After preparation, the blood smears are fixed, stained with Giemsa, and examined under high magnification (1000x).
• 😀 The platelet aggregation is quantified by counting both aggregated platelets and free platelets at three key locations: lateral, medial, and lateral-medial.
• 😀 Results from the control smear showed no platelet aggregation, with a count of 41 free platelets and 0% aggregation.
• 😀 Results from the ADB smear at three minutes showed 30 aggregated platelets and 19 free platelets, with a calculated aggregation percentage of 61.62%.
• 😀 The normal range for platelet aggregation is 50-70%, and the observed result of 61.62% is within the normal range.
• 😀 The video concludes with a call to action to like, share, comment, and subscribe.

Q & A

What is the main purpose of the platelet aggregation test described in the script?

The main purpose is to evaluate the ability of platelets to form aggregates, which is essential in understanding their role in blood clotting and hemostasis.

What are the key materials needed for performing the platelet aggregation test?

The key materials include a glass slide (for smear preparation), citrated blood (to prevent coagulation), an ADB agent (to stimulate platelet aggregation), a microscope, Giemsa staining solution, and a stopwatch.

How is the platelet aggregation test performed?

The test involves preparing a blood smear at two time points: one as a control (without any additives) and one after adding ADB to the blood. After 3 minutes, the blood smear is stained, fixed, and then observed under a microscope for platelet aggregation.

Why is citrated blood used in the platelet aggregation test?

Citrated blood is used because citrates act as anticoagulants, preventing the blood from clotting during the procedure and allowing for accurate observation of platelet aggregation.

What does the term 'platelet aggregation' refer to in this context?

Platelet aggregation refers to the clumping together of platelets, which is an important part of the blood clotting process. In this test, aggregation is stimulated by the addition of an ADB agent, and the aggregation is observed under a microscope.

What does the formula for calculating platelet aggregation involve?

The formula for calculating platelet aggregation is: Platelet Aggregation (%) = (Number of Aggregated Platelets / Total Platelets) * 100. This formula helps determine the percentage of platelets that have aggregated compared to the total number of platelets.

How is the platelet aggregation percentage interpreted in the results?

The platelet aggregation percentage is used to assess platelet function. A normal range of platelet aggregation is between 50-70%. If the percentage falls outside this range, it may indicate a platelet function disorder.

What was the result of the platelet aggregation at 0 minutes (control sample)?

At 0 minutes, which is the control sample without any additives, no platelet aggregation was observed. Only free platelets were visible under the microscope.

What was the result of the platelet aggregation at 3 minutes after adding ADB?

At 3 minutes after adding ADB, platelet aggregation was observed. The percentage of platelet aggregation was calculated to be 61.62%, which is within the normal range of 50-70%.

How is the corrected platelet aggregation percentage calculated?

The corrected platelet aggregation percentage is calculated by subtracting the control sample's aggregation percentage (at 0 minutes) from the experimental sample's aggregation percentage (at 3 minutes), and then dividing the result by 100, as follows: Corrected Platelet Aggregation (%) = [(Aggregation at 3 min - Aggregation at 0 min) / (100 - Aggregation at 0 min)] * 100.