Pengamatan Bentuk Bakteri/Mikroskop Bakteri/Gram staining/Pewarnaan Gram/Apusan Film/microscope

Summary

In this educational video from ilerning.com, a microbiology lab session focuses on observing the shapes of bacteria, specifically in food samples. Students learn to differentiate between bacteria, mold, and yeast through a practical module. The process involves preparing bacterial films, staining with Gram's reagent, and examining under a microscope. The video guides viewers through the steps of bacterial observation, from cleaning and sterilizing tools to applying stains and observing under different magnifications, concluding with the analysis of bacterial shapes and colors.

Takeaways

• 🔬 The lab focuses on food microbiology, specifically observing bacteria, molds, and yeasts.
• 🧫 The practical involves distinguishing between bacterial, mold, and yeast forms.
• 🧴 The work area is cleaned with 70% alcohol before starting the experiment.
• 🧪 The Gram staining process uses reagents like crystal violet, lugol, 95% alcohol, and safranin.
• 🧬 Sterilization of equipment, such as using a flame for the ose (loop), is emphasized to avoid contamination.
• 💧 Aquades (distilled water) is used for rinsing the samples during the Gram staining procedure.
• 🔍 The observation of bacteria under the microscope involves using immersion oil to enhance clarity at high magnifications.
• 🦠 Different bacterial colonies, such as round white and orange colonies, are examined to compare their morphology.
• 🔭 Multiple magnification levels (4x, 10x, 40x, 100x) are used to view the bacteria under the microscope.
• 📋 Students are instructed to document their observations, including bacterial shape and color, in their lab reports.

Q & A

What is the main objective of the microbiology food practice module 2?

The main objective is to enable students or researchers to distinguish the shapes of bacteria, kapang, and khamir.

What are the materials used for observing bacterial shapes in the practice?

The materials include a striker with 70% alcohol for cleaning the workspace, aquades for rinsing staining solution, Gram staining reagents, immersion oil, Bunsen burner, glass cover slips, glass slides, and microbial cultures grown on agar surfaces.

What is the purpose of using 70% alcohol in the practice?

70% alcohol is used to clean the workspace to ensure sterility before handling bacterial cultures.

What are the steps involved in making a bacterial film or smear?

The steps include cleaning the workspace with 70% alcohol, arranging the required tools, igniting the Bunsen burner for bacterial fixation, preparing the bacteria grown on agar, using a cover slip and glass slide, and applying 70% alcohol to clean them.

How is the bacterial film fixed onto the glass slide?

The bacterial film is fixed by passing the glass slide quickly over the flame of the Bunsen burner until all the water or aquades evaporate.

What is the first staining solution used in the Gram staining process?

The first staining solution used is crystal violet.

What is the purpose of the iodine solution in the Gram staining process?

The iodine solution is used to fix the crystal violet to the bacterial cells.

How long is the bacterial film exposed to the iodine solution during Gram staining?

The bacterial film is exposed to the iodine solution for 1 minute.

What is the role of 95% alcohol in the Gram staining process?

95% alcohol is used to decolorize the bacterial film by removing the excess crystal violet and iodine complex.

What is the counterstaining solution used after the decolorization step in Gram staining?

The counterstaining solution used is safranin.

How long is the bacterial film stained with safranin?

The bacterial film is stained with safranin for 10 seconds.

What is the final step before observing the stained bacterial film under the microscope?

The final step is to apply immersion oil and cover with a cover slip, ensuring no air bubbles are formed under the cover slip.