Fouchet's test - for detection of bile pigments in urine
Summary
TLDRThis video demonstrates the procedure for detecting bile pigments in urine using a simple test. It begins by preparing a clean test tube, adding 5 ml of urine, and a pinch of magnesium sulfate. Then, 1 ml of 10% barium chloride is added and the mixture is filtered. Afterward, a few drops of the fashion reagent are sprinkled onto the precipitate, resulting in a green color. This green hue confirms a positive result for bile pigments, specifically indicating the presence of bilirubin, which is crucial in diagnosing liver-related conditions.
Takeaways
- đ A test is being performed to detect bile pigments in urea.
- đ The necessary equipment for this test includes test tubes, a test tube stand, a measuring cylinder, a test holder, and a funnel.
- đ The test involves using magnesium sulfate (10%), barium chloride, and fashion reagent.
- đ First, a clean test tube is required to perform the test.
- đ 5 ml of urine sample is added to the test tube.
- đ A pinch of magnesium sulfate is added to the test tube.
- đ 1 ml of 10% barium chloride is added to the test tube.
- đ The contents are filtered after the reagents are added.
- đ The precipitate is collected after filtration.
- đ A few drops of fashion reagent are added to the precipitate, resulting in a formation of a green color.
- đ The appearance of the green color indicates a positive result for the fashion test.
Q & A
What is the purpose of the test described in the script?
-The test is used to detect the presence of bile pigments in urine.
What equipment is required for this test?
-The required equipment includes a test tube, test tube stand, measuring cylinder, test holder, and a funnel.
What chemicals are used in this test?
-The chemicals used are magnesium sulfate (10%), barium chloride, and fashion reagent.
How is the urine sample prepared for the test?
-A clean test tube is taken, and 5 ml of the urine sample is added to it.
What is the first step after adding the urine sample to the test tube?
-A pinch of magnesium sulfate is added to the urine sample.
What happens after the magnesium sulfate is added?
-1 ml of 10% barium chloride is added to the mixture, and the contents are then filtered.
What is done after filtering the contents?
-The precipitate is taken, and a few drops of the fashion reagent are sprinkled onto it.
What indicates a positive result in this test?
-The formation of a green color after adding the fashion reagent indicates a positive result.
Why is the green color significant?
-The green color signifies the presence of bile pigments in the urine sample, confirming a positive fashion test.
What does the test specifically detect in the urine?
-The test specifically detects bile pigments, which can be indicative of liver or biliary system conditions.
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