Using a Micropipet

Bio-Rad Laboratories
16 Oct 201204:22

Summary

TLDRThis video tutorial instructs on using an adjustable micropipette, a vital tool in molecular biology labs for precise liquid measurements. It covers the pipette's parts, volume ranges, and tips for different applications. The process includes setting the volume, attaching a tip, drawing up liquid to the first stop, and dispensing it completely at the second stop. Specialized tips like aerosol barriers and gel-loading tips are also mentioned.

Takeaways

  • 🧪 **Micropipette Usage**: Micropipettes are essential tools in molecular biology labs for measuring and transferring small liquid volumes.
  • 🔧 **Parts of a Micropipette**: Key components include the plunger button, tip ejector button, volume adjustment knob, digital display window, shaft, and ejector arm.
  • 🌡️ **Volume Range**: Micropipettes have specific volume ranges indicated on the plunger button, with common ranges being 0.5-10 μl, 2-20 μl, 20-200 μl, and 100-1000 μl.
  • 💧 **Tip Selection**: Choose the correct tip size and type for the sample volume and pipette range; standard tips come in three sizes and specialized tips are available for specific purposes.
  • ⚙️ **Setting the Volume**: Adjust the volume by turning the knob until the desired volume is displayed in the digital window.
  • 🔴 **Reading the Display**: Volume is read from top to bottom, with the bottom digit indicating tenths of a microliter for certain pipettes.
  • 📌 **Applying the Tip**: Gently but firmly attach the disposable tip to ensure an airtight seal.
  • 👆 **Plunger Operation**: Press the plunger button to the first (soft) stop for calibrated measurement, and to the second (hard) stop for complete liquid dispensing.
  • 💉 **Sampling Procedure**: Press the plunger to the first stop, insert the tip into the sample tube near the bottom, and release the plunger to withdraw the sample.
  • 🗑️ **Transferring the Sample**: Place the tip into a new microtube and depress the plunger button, pausing at the first stop, then continue to the second stop to expel the sample.
  • 🔄 **Post-Transfer**: While holding the plunger button down, remove the tip from the microtube and release the button once outside.

Q & A

  • What is a micropipette used for in a molecular biology laboratory?

    -A micropipette is used to measure and transfer small volumes of liquid in a molecular biology laboratory.

  • What are the main parts of an adjustable micropipette?

    -The main parts of an adjustable micropipette include the plunger button, tip ejector button, volume adjustment knob, digital display window, shaft, and ejector arm.

  • What does the plunger button on a micropipette do?

    -The plunger button is used to fill and dispense samples.

  • What is the purpose of the tip ejector button on a micropipette?

    -The tip ejector button is used to remove used tips from the micropipette.

  • How is the volume of liquid to be transferred set on a micropipette?

    -The volume is set by turning the volume adjustment knob until the desired volume appears in the digital display window.

  • Where can the volume range for a micropipette be found?

    -The volume range for a micropipette can be found on the top of the plunger button.

  • What are the most commonly used ranges for micropipettes?

    -The most commonly used ranges for micropipettes are 0.5 to 10 microlitres, 2 to 20 microlitres, 20 to 200 microlitres, and 100 to 1000 microlitres.

  • What are the three standard sizes of pipette tips used for general pipetting?

    -The three standard sizes of pipette tips for general pipetting are 0.5 to 10 microlitres, 2 to 20 microlitres, and 20 to 200 microlitres.

  • What is the function of an aerosol barrier tip?

    -An aerosol barrier tip has a sterile filter to prevent contamination when transferring samples.

  • How should you hold the micropipette when obtaining a sample?

    -You should hold the micropipette with your thumb on the plunger button and wrap your fingers around it.

  • What does the first or soft stop on the plunger button represent?

    -The first or soft stop on the plunger button represents the calibrated measurement of the volume set on the micropipette.

  • How do you complete the transfer of a sample using a micropipette?

    -To complete the transfer of a sample, place the micropipette tip into a fresh microtube, slowly depress the plunger button, pause at the first stop, then continue depressing until the second stop.

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Étiquettes Connexes
Molecular BiologyLab TechniquesMicropipette UseLiquid HandlingLab EquipmentBiology LabVolume ControlLab SafetySample TransferLab Protocol
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