Muestreo de Superficies

Food Safety Panama

5 Apr 202006:08

Summary

TLDRThis video script outlines the process of surface sampling for microbial counts, specifically targeting 'Staphylococcus aureus'. It emphasizes the importance of using a template for consistent size to compare microbiological loads in a defined area. The script details the preparation of materials such as gloves, a sponge with a sterile bag, swabs, and a chain of custody form. It also describes the steps for sampling, including wearing protective gear, labeling the sterile bag, and using a sponge to collect samples from both smooth and irregular surfaces. The process involves careful handling, ensuring proper labeling, and storage in a pre-chilled container. Additionally, it covers hand sampling techniques using a swab moistened with neutralizing broth, ensuring thorough coverage of the entire hand.

Takeaways

🔬 The script discusses surface sampling for counting indicators such as 'Staphylococcus aureus'.
📏 The importance of using a template for surface sampling to compare microbiological loads in a defined area is highlighted.
📝 It is necessary to inform the laboratory of the template size for consistent sampling and comparison.
🧤 Materials required for sampling include gloves, a sponge with a sterile bag, a swab, a disinfectant solution, a template, a chain of custody form, and a thermal container with cold packs.
👷‍♂️ Personal protective equipment like a face shield and mask are essential for avoiding sample contamination.
📝 Labeling the sterile bag with sample information using a permanent marker is a crucial step.
🧽 For smooth surfaces, a template is placed and a sponge is used to rub the area to collect samples.
🔄 Both sides of the sponge are used to ensure complete coverage of the sampling area.
🧊 After sampling, the sponge is placed in a sterile bag, labeled, and stored in a pre-chilled thermal container.
🦠 When searching for pathogens on smooth surfaces, a template is not necessary, and the goal is to find any bacterial pathogen present.
🌡 For irregular surfaces, a swab with neutralizing solution is recommended to reach nooks and crannies.
👐 Hand sampling involves labeling with the person's name or employee number and using a swab moistened with neutralizing solution to cover the entire palm, between fingers, and under the nails.

Q & A

What is the purpose of using a template when sampling surfaces for microbiological counts?
-The purpose of using a template is to compare the microbiological load in a defined area, ensuring consistency in the size of the area sampled for accurate comparisons.
Why is it important to inform the laboratory about the size of the template used for sampling?
-Informing the laboratory about the template size allows them to include this information in the report, which is crucial for maintaining a record of samples and comparing results over time.
What materials are necessary for surface sampling as described in the script?
-The necessary materials include gloves, a swab, a sterile sponge with its sterile bag, a disinfectant solution, a template, a chain of custody or log, a pre-disinfected container with refrigerants, and hand sanitizer.
How should one prepare before taking a surface sample to avoid contamination?
-One should wear gloves, a mask, and put on a sterile gown. After washing hands and applying hand sanitizer, they should put on a new pair of gloves before handling the sterile sponge.
What is the correct procedure for sampling a smooth surface like a table?
-Select the area to be sampled, place the template on the surface, and carefully move it around to avoid contamination. Then, press the sponge against the surface within the area defined by the template to collect the sample.
How should the sponge be handled after collecting the sample from a smooth surface?
-The sponge should be placed in its sterile bag, ensuring it is properly labeled, and then stored in a pre-chilled container with refrigerants.
Why is a template not necessary when looking for pathogens on a surface?
-A template is not necessary because the goal is to find the presence or absence of pathogens, and any bacteria present on the surface is of interest, not just those within a defined area.
What is the recommended tool for sampling irregular surfaces?
-The recommended tool for irregular surfaces is a swab with neutralizing broth, which can reach small and hard-to-access areas, such as crevices.
How should hands be sampled for microbiological analysis?
-Hands should be sampled by breaking the seal of the swab to ensure the broth is wet, then rubbing the entire palm, between the fingers, and under the nails with the swab, ensuring full coverage.
What should be done after collecting a hand sample to ensure proper handling?
-After collecting the hand sample, the swab should be placed back into its container, sealed, and properly labeled before being stored.

Outlines

00:00

🔬 Surface Sampling for Microbiological Analysis

This paragraph describes the process of conducting surface sampling for microbiological counts, specifically for 'Staphylococcus aureus'. It emphasizes the importance of using a template to compare microbial loads within a defined area, regardless of its size. The script details the necessary materials, including gloves, a sponge with a sterile bag, a disinfectant swab, a transport chain or log, and a pre-chilled container with refrigerants. It also outlines the steps to avoid contamination, such as wearing a mask and gloves, labeling the sterile bag with sample information using a permanent marker, and applying alcohol after handwashing. The sampling technique involves rubbing a sponge within the area defined by the template and ensuring both sides of the sponge are used to cover the entire surface. The sponge is then placed in a sterile bag, properly labeled, and stored in a pre-chilled container. The paragraph also discusses the use of a swab for irregular surfaces and the need for a neutralizing solution.

05:01

👐 Hand Sampling Technique for Microbiological Assessment

The second paragraph focuses on the hand sampling technique used to assess the presence of microorganisms on human hands. It begins by detailing the labeling process with the person's name or employee number and the purpose of the sampling. The procedure involves breaking the seal of the swab to ensure the neutralizing solution is moist, and then rubbing the swab across the entire palm, between the fingers, and under the nails to ensure thorough coverage. The swab is then reinserted into its container and sealed. The paragraph highlights the importance of proper labeling and storage of the samples to maintain the integrity of the microbiological assessment.

Mindmap

Keywords

💡Surface Sampling

Surface sampling refers to the process of collecting samples from a surface for the purpose of analysis, such as microbiological examination. In the video's context, it is a method used to count indicators like 'Staphylococcus aureus'. The script mentions using a template for surface sampling to ensure consistency in the area being tested, which is crucial for comparing microbiological loads across different samples.

💡Template

A template in this context is a tool used to standardize the area from which the surface sample is taken. The script emphasizes that the size of the template does not matter as long as it is consistent across samples. This ensures that the laboratory can include the size information in their reports, which is essential for accurate comparison of microbiological results.

💡Microbiological Load

Microbiological load refers to the quantity of microorganisms present in a given area or sample. The script discusses comparing the microbiological load in a defined area, which is a key aspect of assessing cleanliness or contamination levels. The use of a template helps in maintaining a consistent area for sampling to accurately measure this load.

💡Sterile Sponge

A sterile sponge is used in the sampling process to collect microorganisms from a surface without introducing contaminants. The script describes carefully removing the sponge from its packaging to avoid contamination and using it to rub the defined area within the template to collect the sample.

💡Sterile Bag

A sterile bag is used to store the collected samples to maintain their integrity and prevent contamination until they can be analyzed. The script mentions placing the used sponge into a sterile bag after sampling and ensuring it is properly labeled, which is crucial for traceability and accuracy in laboratory testing.

💡Thermometer Container

A thermometer container is used to store samples at a controlled temperature, which is important for preserving the condition of the microbiological samples. The script refers to placing the sample in a pre-chilled container with refrigerants to maintain the necessary temperature for transport to the laboratory.

💡Hand Hygiene

Hand hygiene is a critical practice in the sampling process to prevent cross-contamination. The script mentions using an alcohol-based hand sanitizer before handling the sample, which is a standard procedure in microbiological sampling to ensure that the sampler's hands do not introduce additional microorganisms.

💡Indicator

In the context of microbiology, an indicator refers to a specific microorganism or chemical substance that is used to measure the presence of other microorganisms or conditions. The script specifically mentions 'Staphylococcus aureus' as an indicator for which the surface sampling is being conducted.

💡Irregular Surfaces

Irregular surfaces pose a challenge for surface sampling because they have uneven textures and crevices. The script discusses using a swab with neutralizing broth for such surfaces, which can reach into small and hard-to-access areas, ensuring a thorough collection of microorganisms.

💡Swab

A swab is a tool used for collecting samples, especially from irregular surfaces or areas that are difficult to reach with a sponge. The script describes using a swab with neutralizing broth to apply pressure and collect samples from the entire area, which is important for obtaining a representative sample.

💡Chain of Custody

The chain of custody is a record of the history and handling of a sample from the time it is collected to when it is analyzed. The script mentions the importance of labeling and recording information about the sample to maintain the integrity of the chain of custody, which is crucial for the reliability and legal admissibility of the test results.

Highlights

Demonstration of surface sampling for counting indicators such as 'Staphylococcus aureus'.

Use of a template for comparing microbiological load in a defined area.

Importance of consistent template size for accurate comparison of samples.

Indicating template size to the laboratory for report inclusion.

Preparation of materials including gloves, swabs, and sterile sponge.

Utilization of a 'you swap' with letting broth for hygiene.

Use of an external template, chain of custody, and a pre-disinfected thermal container.

Hand hygiene with alcohol before starting the sampling process.

Wearing a mask and gloves to avoid sample contamination.

Labeling the sterile bag with necessary sample information using a permanent marker.

Selection of area for sampling on smooth surfaces like a table.

Use of both sides of the sponge to collect microbiological samples.

Placing the sponge in a sterile bag and ensuring it is properly labeled.

Storing the sample in a pre-cooled thermal container with refrigerants.

Sampling for pathogens without the need for a template.

Use of a swab to collect samples from irregular surfaces.

Recommended use of a swab with neutralizing broth for hard-to-reach areas.

Hand sampling procedure using labeled swabs with individual or employee identification.

Ensuring the swab is moistened with neutralizing broth before hand sampling.

Thorough rubbing with the swab to cover the entire palm, between fingers, and under the nails.

Proper sealing and labeling of the swab after hand sampling.