Labeling and plate terminology.

Summary

Dans ce script de vidéo, l'orateur aborde divers termes relatifs à la culture de champignons, tels que T pour transfert, C pour clone, F pour génération filiale, Ms pour multipore et WT pour wild type. Il explique que T0 signifie départ à partir de spores, tandis que T1, T2, T3, etc., indiquent les différentes générations de transferts successifs. L'orateur discute également de la signification des nombres de passage (P), bien que ceux-ci ne soient pas largement utilisés dans la communauté mycologique à domicile. Il souligne l'importance de la stabilité génétique et comment les nombres F sont utilisés pour indiquer le niveau de génération après la formation de spores. Le contexte de la culture de champignons est riche en jargon et nécessite une compréhension des termes pour naviguer efficacement dans cette communauté. L'orateur mentionne également l'intention de produire d'autres vidéos pour approfondir certains sujets, reconnaissant la complexité des termes et des concepts abordés.

Takeaways

• 📝 T0 signifie que l'on commence avec des spores et peut être noté comme T1 si on part d'une source sauvage.
• 🔄 T1 représente la première transfert ou sous-culture d'une plaque de spores vers une nouvelle plaque.
• 🛑 La terminologie F (filial) fait référence aux générations successives, où F1, F2, etc., indiquent le nombre de cycles de meïose subis.
• 🧬 L'acronyme C (clone) est utilisé pour identifier des clones issus d'une même source, comme C1, C2, etc.
• 💧 Le passage (passaging) décrit la croissance de mycélium jusqu'à la bordure d'une plaque avant de procéder à un transfert.
• 🔬 WT (wild type) fait référence à un organisme issu de l'environnement naturel, sans modification génétique.
• 🍄 Ms (multipore) indique qu'une spore ou un ensemble de spores provient d'une source à plusieurs pores.
• 📏 Les numéros de passage (P numbers) sont moins couramment utilisés dans la culture de champignons mais peuvent être trouvés dans les anciens textes.
• ✋ Il est important de comprendre sa propre système de notation pour les plaques, même si d'autres personnes ne sont pas obligées de la comprendre.
• 🔠 L'utilisation d'acronymes est fréquente dans la culture de champignons pour des raisons de commodité et de concision.
• 🌱 La stabilisation d'une souche est un sujet de débat, certains pensent qu'elle ne doit être nommée qu'à partir de F5 ou F6, bien que cela ne soit pas universellement accepté.
• ❗ Il est crucial d'utiliser des spores pour passer d'une génération à l'autre et d'augmenter le numéro F après chaque cycle de meïose.

Q & A

Quelle est la signification de l'abréviation 'Ms' dans le contexte de la culture de champignons?

Dans le contexte de la culture de champignons, 'Ms' signifie 'multipore', faisant référence à un type de seringue utilisée pour la propagation des spores.

Comment la génération filiale (F) est-elle utilisée pour identifier les différentes générations de cultures de champignons?

La génération filiale (F) est utilisée pour suivre le nombre de cycles de meïose qu'une culture a traversé. Par exemple, F0 fait référence aux spores issues de la nature, tandis que F1, F2, F3, etc., représentent les générations successives après chaque cycle de meïose.

Quels termes sont souvent confondus ou utilisés de manière interchangeable pour décrire des cultures de champignons?

Les termes 'strain' (souche) et 'isolate' (isolat) sont souvent confondus ou utilisés de manière interchangeable pour décrire des cultures de champignons, bien que 'strain' dans le langage courant pourrait faire référence à une souche spécifique, tandis qu'un 'isolate' est plus techniquement correct pour décrire un clone.

Que signifie l'abréviation 'WT' et dans quel contexte est-elle utilisée?

L'abréviation 'WT' signifie 'wild type' et est utilisée pour décrire un organisme ou une souche qui a été isolée de la nature et qui n'a pas été modifiée génétiquement. C'est un terme couramment utilisé en biologie pour comparer les caractéristiques d'une souche sauvage avec celles d'une souche modifiée.

Quelle est la différence entre un 'transfer' (T) et un 'passage' (P) dans le contexte de la culture de champignons?

Un 'transfer' (T) fait référence à l'acte de déplacer un échantillon de mycélium d'une plaque à une autre, tandis qu'un 'passage' (P) implique généralement de laisser le mycélium pousser jusqu'au bord de la plaque pour effectuer des calculs sur le nombre de divisions mitotiques qui se sont produites. Le terme 'passage' est moins couramment utilisé que 'transfer' dans la culture de champignons.

Comment les numéros de passage (P) sont-ils utilisés dans la culture de champignons?

Les numéros de passage (P) ne sont pas couramment utilisés dans la culture de champignons de loisir, mais peuvent être rencontrés dans la littérature ou la recherche plus poussée. Ils permettent de suivre le nombre de fois qu'un mycélium a été laissé pousser jusqu'à saturation avant d'être transféré.

Que signifie l'abréviation 'C' et comment est-elle utilisée pour identifier les cultures de champignons?

L'abréviation 'C' signifie 'clone'. Elle est utilisée pour identifier des cultures de champignons qui sont des copies exactes de la culture parente, généralement obtenues par des méthodes telles que la culture de tissus ou la propagation de spores.

Quels facteurs peuvent compliquer l'utilisation des numéros de génération filiale (F) dans la culture de champignons?

L'utilisation des numéros de génération filiale (F) peut être compliquée par le fait que les champignons ne forment pas de diploïdes sous forme de mycélium, ce qui entraîne une certaine confusion dans l'attribution des numéros F. De plus, la stabilité génétique n'est pas garantie à un certain stade F, ce qui peut causer des débats sur la stabilité et la nomination des souches.

Comment les numéros de génération filiale (F) sont-ils associés aux spores et à la meïose?

Les numéros de génération filiale (F) sont associés aux spores et à la meïose car chaque génération de spores qui résulte de la meïose (une division cellulaire sexuée) est considérée comme une nouvelle génération filiale. Par exemple, des spores collectées à partir d'une culture F2, lorsqu'elles sont germées, produiront une culture F3.

Quelle est la différence entre un 'multipore syringe' et un 'monoculture'?

Un 'multipore syringe' est un outil utilisé pour la propagation de spores de champignons, généralement contenant plusieurs pores pour permettre le passage des spores. Un 'monoculture', en revanche, fait référence à une culture qui contient une seule souche ou espèce de microorganisme, utilisée pour des études spécifiques ou pour éviter les contaminations.

Comment la date est-elle enregistrée dans le système de notation des cultures de champignons?

Dans le système de notation des cultures de champignons, la date est enregistrée après le numéro de génération et le type de culture, généralement sous la forme 'jour mois année'. Par exemple, une culture notée 'F2 Ms T1 09 May 2024' indique qu'elle est une souche multipore (Ms) de la première transfert (T1) de la deuxième génération filiale (F2), enregistrée le 9 mai 2024.

Outlines

00:00

📝 Introduction aux termes de culture de champignons

Le présentateur aborde divers termes liés à la culture de champignons, soulignant l'utilisation de termes spécifiques tels que 'multipore' (Ms) et 'wild type' (WT). Il explique les abréviations telles que F pour 'filial', P pour 'passage', et T pour 'transfer'. Il insiste sur la signification de T0 comme étant le départ à partir de spores et décrit le processus de transfert de culture de T0 à T1, T2, etc.

05:01

🔄 Explication des termes T, C et F

Le présentateur poursuit avec une explication plus approfondie des termes T pour 'transfer', C pour 'clone', et F pour 'filial'. Il détaille le processus de marquage des plaques de culture, y compris l'identification des différentes générations de clones et le suivi des transferts. Il mentionne également l'utilisation de la date pour identifier les cultures.

10:02

🌱 Cycle de vie des cultures et génération F

Ici, le présentateur explique le cycle de vie des cultures de champignons et l'importance de la génération F, qui fait référence aux générations filiales ou aux frères et sœurs. Il aborde la notion de stabilisation des cultures à partir de F5 et comment les spores jouent un rôle clé dans l'incrémentation de la génération F.

15:06

📚 Autres termes clés et pratiques de marquage

Le présentateur mentionne d'autres termes tels que 'multipore land race', 'legacy', et 'mono Caron'. Il insiste sur l'importance de la compréhension personnelle des termes et du système de marquage utilisé, soulignant que cela n'est pas essentiel pour les autres à moins de vendre ou d'échanger des plaques. Il conclut en disant qu'il faudra peut-être traiter ces termes avec plus de détails dans des vidéos ultérieures.

20:07

📹 Considérations pour la production de vidéos éducatives

Enfin, le présentateur réfléchit à la longueur de sa vidéo et décide qu'il faudra peut-être diviser le sujet en plusieurs vidéos pour une meilleure compréhension. Il mentionne également les aspects techniques de l'enregistrement et de la publication de la vidéo sur YouTube et invite les spectateurs à poser des questions dans les commentaires.

Keywords

💡Multipore

Multipore fait référence à un type de seringue utilisée dans le monde des champignons pour inoculer des spores. Dans le script, il est mentionné que 'Ms' (multipore syringe) est souvent utilisé pour désigner une seringue de ce type, qui est un outil clé dans la culture de champignons.

💡Wild Type (WT)

Wild Type (ou WT) est un terme utilisé pour décrire un organisme ou une souche qui n'a pas été modifié génétiquement et qui représente la forme la plus commune dans la nature. Dans le contexte du script, il est suggéré que ce terme pourrait être utilisé pour décrire des souches de champignons issues de l'environnement naturel.

💡Filial Generation (F)

Filial Generation (F) est une notion utilisée pour décrire les générations successives d'un organisme après la reproduction sexuée. Dans le script, il est expliqué que les nombres F (F1, F2, etc.) sont utilisés pour suivre les générations de cultures de champignons à partir des spores, où F0 pourrait représenter les parents ou la souche sauvage.

💡Clone (C)

Clone (C) fait référence à une copie exacte d'un organisme ou d'une partie d'un organisme. Dans le contexte du script, le terme est utilisé pour désigner des cultures de champignons qui sont issues de la même source et partagent donc les mêmes caractéristiques génétiques, comme illustré par l'utilisation de C1, C2, C3 pour représenter les différents clones.

💡Transfer (T)

Transfer (T) est un terme utilisé pour décrire l'acte de déplacer ou de transférer une culture d'un milieu à un autre. Dans le script, les nombres T (T0, T1, T2, etc.) sont utilisés pour identifier les différentes étapes de transfert ou de sous-culture d'une souche de champignon, où T0 pourrait signifier le début avec des spores et T1, T2, etc., les transferts successifs.

💡Monocaron

Monocaron (M) est un terme qui pourrait être utilisé pour décrire une culture de champignons où une seule souche a été isolée et cultivée. Dans le script, il est mentionné que M1, M2, etc., pourraient être utilisés pour identifier les différentes générations d'une telle culture monoclone.

💡Isolate (ISO)

Isolate (ISO) est un terme qui désigne un échantillon ou une souche qui a été isolée d'une population pour être étudiée ou cultivée séparément. Dans le script, il est indiqué que l'on pourrait utiliser ce terme pour parler d'une souche de champignon qui a été isolée et cultivée, similaire au concept de clone.

💡Subculturing

Subculturing est le processus de transfert d'une partie d'une culture microbienne à un nouveau milieu de culture. Dans le script, il est expliqué que ce terme est souvent utilisé pour décrire le transfert de mycélium de champignon d'une plaque à une autre, ce qui est essentiel pour la propagation et la maintenance de cultures de champignons.

💡Passage

Passage fait référence au nombre de fois qu'une culture a été transférée ou subcultivée. Dans le script, il est mentionné que, bien que le terme passage soit moins utilisé dans la communauté des mycologues amateurs, il implique la croissance d'une culture jusqu'à ce qu'elle atteigne les bords d'une plaque avant d'être transférée, ce qui permet de suivre le nombre de divisions mitotiques.

💡Land Race

Land Race est un terme utilisé pour décrire une variété de plante qui a évolué naturellement dans une région particulière et qui a été sélectionnée par les conditions locales. Dans le script, il est discuté que ce terme est moins applicable à l'heure actuelle pour les cubes de cannabis et que le terme 'Legacy' pourrait être plus approprié pour décrire les souches héréditaires.

💡Legacy Cultigen

Legacy Cultigen fait référence à une variété de plante qui a été cultivée pendant de nombreuses générations et qui a une histoire et des caractéristiques distinctifs. Dans le script, le terme est suggéré comme une alternative à 'Land Race' pour décrire des souches de cannabis ou d'autres plantes qui ont une lignée longue et bien établie.

Highlights

Introduction to various terms used in mycology such as T (transfer), C (clone), and F (filial).

Explanation of T0 as starting with spores and the process of labeling agar plates in mycology.

Differentiation between transfer (T) and passage numbers in the context of mycelial growth and subculturing.

The concept of 'wild type' (WT) and its relevance to strains isolated from their natural environment.

Discussion on the use of the term 'multipore syringe' (Ms) and its significance in mycology.

Clarification of the term 'clone' (C) and its application in the process of subculturing mycelium.

Importance of understanding one's own labeling system for in-house use rather than adhering to external standards.

Insight into the controversy surrounding the use of F numbers and the stabilization of strains in mycology.

Explanation of the term 'monoculture' (M) and its distinction from other terms like 'multipore'.

Discussion on the practicality of raising the F number after spore germination and the assumption behind this practice.

Differentiation between the terms 'strain' and 'isolate' and the context in which they are used interchangeably.

The process of stabilizing a strain through successive filial generations and the common misconceptions regarding this process.

Use of the term 'legacy' in reference to older or heirloom strains and its advantages over 'landrace'.

Advice on starting with T1 for labeling when beginning with a wild source and the flexibility of choosing different starting points.

The significance of spores and meiosis in the process of advancing filial generations in mycology.

Differentiating between grain-to-grain transfers (G generations) and the labeling system used for spawn expansion.

Acknowledgment of the complexity of mycology terminology and the need for further detailed explanations in subsequent videos.

Transcripts

hello everybody I wanted to go through

some terms today uh looks like the

things the recording uh I wrote all

these up here I don't know I was kind of

hesitant maybe I do need a bigger a

bigger whiteboard uh but this is

tpcf Ms and WT uh before I forget WT

stands for wild type Ms stands for

multipore often there's a multipore

syringe like an S um and there can be

various other letters in this a BRI

viation but MSS or Ms that stands for

multipore I'll come back to that here in

a second but for right now I want to

start at the top so F stands for filial

Generation Um that's F let's see I

probably should have wrote some of this

stuff down here might have to erase some

of this stuff so I'm going to write it

now before I forget um for clarity P

stands for passage and I'm going to get

to that in a second so I won't write it

t stands for transfer okay and there's a

bunch of other words that I'm going to

throw in so transfer or

subculturing uh is what we often

use when we're making essentially when

we take an agar plate uh and we transfer

a piece to a new plate so it's just a

generic word the rest of the world uses

the term subculture say so if you grow

Mamon uh tissue culture like in in agar

plates usually in liquid media we we

usually refer to that subculturing but

for some reason transfer hence the T has

has kind of caught on in the mological

IC community at least the home mological

Community people have all sorts of other

slang words but t on plates if you see a

t like a T1 T2 T3 um or a t0 so let me

let me just start there so t let me

switch so what does t0o mean I'm going

to have to erase a lot of this stuff you

guys my uh aggar or my aggar my uh

whiteboard just basically isn't uh big

enough to write all this stuff and I

don't even I don't even know if I need

to go bigger so I'm going to write these

things and then I'll probably go back

erase them also helps to sort of clear

my thoughts to move on the next topic so

t0 t0 means essentially you are starting

off with spores okay so when you have a

Spore print or even maybe a multi-pore

syringe uh you shoot it under an agar

plate and you label it t0 so the t0 it

might have been streaking it might have

been a grab and drag so t0 without

getting way way way in depth here

because all of these terms are loaded

with uh kind of special cases and and

uses and and different people use them

in slightly different ways some times t0

is essentially spores so you put spores

on an Agger plate okay so what is T1 so

T1 when you take um a transfer from that

plate of spores or anything else let's

say somebody sends you a a a die carry

on on a plate you could if you wanted to

call that t0 like so that would be like

your master culture or whatever again I

don't really like that that term Master

culture not only do have negative kind

of connotations historically but it also

I it just sounds kind of like very very

like in the box like this is the only

culture I ever use and that's often not

the case uh when you start getting into

into this you'll you'll realize that

there is no such thing as like you know

a master like blue Vuitton or a master

GT they just don't exist so for various

reasons which could take hours to

explain um but T1 means essentially

you've taken your aggar plate and you

cut off a little piece you know let's

say that's your t0 so you take your

little square your Ager Punch or

whatever you want to use and you put it

onto a new plate

mhm so that would be your T1 simple as

that so you do that again then you got

your T2 Etc sorry guys I'm writing again

at a kind of an awkward angle here so if

my my uh my my so um subculturing so we

use in the in the rest of the world they

talk about passage numbers so here's

where things get a little bit tricky

because passages H I believe in Paul

stam's old old books you know the the

mushroom cultivator the TMC and uh what

is it was H growing Gourmet and

medicinal mushrooms the ggmm not the mmm

mmgg which is magic mushroom Growers

guide so the Paul stamitz book um the

the growing Gourmet and M and medicinal

mushrooms uh he uses the term passage so

it's it's kind of a little bit

unfortunately it's kind of a slightly

different concept uh than than transfer

transfer is literally it just means a

transfer um so let's let's say if your

your plate here let's just say you had

your mycelium you know and you take you

can take a transfer at any time you know

your uh your little mycelial uh spot

here might only be you know maybe a

centimeter half an inch wide so that

would be appropriate to say you know

you're transferring it and go from t0 to

T1 Etc passage is a little bit different

because passage kind of implies that

you've let this melium grow to the edge

of the plate so you can do some math and

figure out how many mitotic divisions

have occurred so that's where kind of

Passage it's like you're passaging the

edge of this so and then so it gets a

little bit more complicated uh if you

take your transfer from here or you take

your transfer from here uh you know and

you make a new plate this is kind of a

different scenario because that melium

right there is older it's obviously gone

through more mitotic division so then we

get back into the idea of sidence I

don't even want to get that's a whole

other video in it of itself um so Paul

Stam it sometimes it's p number like

Paul um it actually refers to passage um

it's it's an old term that people use

you know just like you're crossing the

Atlantic or whatever you know from

Europe to America it's a passage um so

again that gets into syence and all

kinds of stuff that's not the topic of

this video because otherwise I'm trying

to make this about 15 minutes and it'll

uh it'll turn into three hours very very

shortly if I if I'm not

careful so let's move on so let's just

forget about that one so we got that one

done okay there you go done well that's

really enough again I could have made

whole videos about each one of these

things I just want to know um I just

want to you know when you see a plate

that says you know

t0 you know Ms I always label mine Ms

too because my multipore I know it's t0

but I put Ms just so I don't forget and

you might see something like uh well

this would probably you wouldn't put an

F number we'll get down to that in a

second here um that's a little bit more

complicated but let's let's just stick

with the basic um for right now so let's

add another thing here let's say you put

uh you got a C on there so you've got t

w okay this is look this let's not make

things um a little bit more complicated

than you need to be um you know the most

important thing in all of this you guys

is that you understand what it means on

your plates uh unless you're selling

plates or trading plates it's not really

necessary for other people to be honest

to understand what your your system is

as long as you understand it remember

this is for your inh house use um so

however you want to do this it's up to

you really people get in really really

heated debates you know on online about

like oh you know especially the F number

that's like a whole whole whole like

controversy there um I may get into that

a little bit later I'll probably try to

stay away from it so let's say you got a

uh let's say you have a plate disbled T3

okay that means transfer three so that

means you know wherever you got it from

maybe you got a plate and you've

transferred it so you got a you could

call it a t0 plate your bu gave you that

it's T1 T2 T3 or he might have had a

label on it already or she right maybe

they gave you a plate where that was a

label T2 and you subcultured it or you

you transferred it so you let make it

T3 um so it would be let's say T3 and it

has says something like F4

C1 okay so obviously let's just let's

just use blue Vuitton for since I that's

the only one that's popping in my head

right now and a date uh today I believe

is the 9th so it's May 9th I use

um I I use the the the kind of rest of

the world use it goes date month year so

this would be May May 9th 2024 what the

hell does all this mean okay so again I

made I might could have started with

this but let's say you got this covered

T's already um f we'll get to in a

second so the C1 what does that mean

well C stands for

clone okay so I didn't write it should

be fairly obvious right uh just like in

the Cannabis world you know you cut off

top of a apical marem or or whatever

you're doing you know it could even come

from a tissue culture that's a whole

other thing I want to get into later you

know you can tissue culture and grow uh

tissue explants from Cannabis and all

kinds of other plants in the same way we

do fungi so once you learn how to do

this in fungi it's equally applicable to

mamalian cell culture as well as um you

know plant culture plant tissue culture

so that kind of is another broad term I

don't know what people like to call it

but you may hear the term like tissue

culture or t C this stands for

tissue

culture okay so if you hear that word TC

or if you see it in the literature um

that's what it refers to so we love

acronyms man I I hate you know I kind of

hate it but they're they're just kind of

a necessity because if I keep saying

tissue culture you know to somebody who

already knows I mean you know TC is

tissue culture they're going to get kind

of like frustrated be like why don't you

just say TC or if I keep saying transfer

three the third transfer you know it's

kind of hard to write it on plate too so

that's part of the I mean it's just

convenient right we need to we only only

not sew you know maybe 3 in or 90 mm or

whatever to write everything uh on a

plate and you know maybe your Sharpie is

a little bit duller than it should be

and you know you start writing this

stuff and you're like who uh some people

again I should go back to that some

people like um you know some people like

writing on the edge of the plate I don't

suggest that you guys I would put it

right up on the top so as an example

here now no I got a a Dion from somebody

and I grew that out um and so I just

labeled that arbitrarily I oh I hope

that focusing things um I I was so

instead of writing C1 I wrote actually

F1 clone I don't know I was feeling

verbose that day um so there's like six

little pieces of of tissue there I don't

know how well you can see that but

they're they're growing out nicely and

so later on so I could call this you

know nuk F1 C1 t i could Pro I would

probably call it T1 um and then maybe

the next one I call T2 you can call it

t0 if you want I don't know it's really

up to you um but anyways uh I was I know

exactly what this is again that's that's

kind of a a good point is that like if

you I only have one nanuk culture or

nanuk um I think this is from Dave I

only have one of these so I don't have

to get all like and I know it's from

Dave he gave it a name I'm gonna I'm

going to call it in a Nook like so I

don't need to really worry about F

numbers and all that later but you

know my next plate when I get spores

I'll get back to that so that when I get

spores uh when I fruited it and get

spores from this this will become an F2

and then I'll drop the drop the Clone

part but before we okay I should

probably I just saw another one of my

plates you might see M okay so

m equals

monoculture uh monoc Caron oops so M is

for monoc Caron I just saw that on one

of my plates over there and I don't want

to get into that again in this video

kind of did that on the last videos but

if you see like an

M1 um or M2 or whatever that refers to

Mono Caron but I don't really want to

get into that today that's a whole

different

topic um so clone so c means clone so

you could have C2 C3 let's say you ran a

multiport tub and you know you might get

six or seven phenos another word I don't

want to get in but phenotype and

genotype that's another whole video um

so so that that P or whatever you know

that that's for passage we don't really

use that so let me just get rid of that

right now we don't really use passage

numbers unless you're Paul stamitz or

one of the people that works for funai

perfectti we don't really use P numbers

um so we just stick with

T's um there's some historical reason

for that but again I don't need to get

into that so we' we've covered C clone

so what about these other terms ISO ISO

is short for isolate so an isolate or a

clone I these kind of terms usually get

kind of used

interchangeably and then for people

especially who are first starting out

the The Strain you might say BV uh

strain has a more specific meaning

strain in in most of the rest of the

biological world is synonymous with

isolate so let's you have a let's say

you have a strain of ecoli that's

pathogenic um now maybe a strain might

be in multiple places so you know if

somebody gets uh well you get sick from

the from from the salad bar and somebody

else gets sick from sick from The Burger

Joint somebody else gets sick from The

Shake Shop you might say that's you know

the strain of eoli what what what is it

I forgot the name of that 189 or

whatever it was um whereas in in the

mushroom world at least an ISO generally

means an isolate so that's kind of

synonymous with clone people will often

sometimes mix these things and it's it's

kind of you got to guess kind of how

they're using it uh I that's why I

adopted the term cultigen so in the

mushroom World strain used to be

something like a B+ a GT a blue Vuitton

on that terminology is kind of faded a

little bit because strain normally

refers to a a particular isolate of a

particular

cultigen and then again see how this is

already getting very very very messy so

I would say the cultigen for this is

blue Vuitton it's the second clone that

I took from a maybe a multiport tub so

I've grown fruit and I've Clon those

fruits and obviously I would have a C1

and then this is my C2 and my C3 I don't

know unless you want to start numbering

back CS or whatever and you call it

anything you want uh and the F4 that

would be the generation so let me get to

that next so what the hell so I'm just

going to erase that you guys so I don't

I keep backtracking and I want to say

more about these things but let me just

uh erase these so I don't I don't like

divert my attention here so what the

hell is an F number F stands for filial

filial it means literally it means

brothers and sisters or siblings so let

me just write siblings so we don't got

to all into that gender

let me say just

siblings um so what that means it's just

literally it's a is an old uh term that

means siblings I think it's Greek or

Latin or something so F4 um F4 means

essentially it's gone through four

cycles of kind of uh meiosis so what

happens say your mom and dad you get

together you have a bunch of brothers

and sisters you would call those your

siblings now mostly in the in the plant

world and the animal world we talk about

things like F0 so F0 typically equals

your parents okay and I've noticed this

this is where I know some people are

going to get like really excited about

this the parents are usually referred to

as the

fzero okay so the parents or things that

were wild okay so let's go so say you go

out in the I don't know in some field in

Texas and you pick up a cube or whatever

um you come come back and you clone it

right so you could call that your F0 and

you would that would imply to me that

that was something that you got from the

wild so you could have a plate that

would say like F0 C1 C2 C3 multiple

clones from from fruit uh and then you

could call that your t0 or your T1 if

you wanted to I I tend to like put one

like T1 because usually you're starting

from some sort of wild Source um so I

like I like the transfer I usually start

at T1 but if you want to start it t z

you can uh and again if you want to do

any if there's going to be a lot of

people that will disagree with this I

don't really care um they shouldn't care

because whatever you understand as your

system uh is the right way this is one

of those where there's like there's

really no right way uh and anybody who

tells you there is is full of

right um so I'm just telling you the the

general way and the the kind of most

accepted kind of standard way that we do

this stuff at least in the home myology

community so when you get to so how do

you get so a lot of people say like well

you need to stabilize something right

like how you going to stabilize right

you need to stabilize you need to get it

to you know your F5 or whatever to

stabilize it you know F5 F6 you can't

give it a name because it's not

stabilized that's you guys

because if you see certain very very

famous culturs floating around uh now

they're at f8 F9 F10 and they're still

not stable you guys so um if you're on

an F1 and F2 you want to give it a name

people are going to give you for it

but you know that's very very common in

the mushroom Community we're kind of

used to it after a while um so what the

hell is F5 mean it means filial

Generations so this is where a little

bit of the controversy comes into

because let's say you get a plate uh

let's say it's let say this is

F2 you want to get it to F3 or F4 F5 how

the hell do you do that well the

essential thing here is spores you

guys spores and

meiosis okay so here's the basic idea if

I start off with something let's just

say it's an F2 so I get a plate it's F2

I make some spawn I grow that out and I

get spores okay when I germinate those

spores I can now say that that's F3 this

is where it gets a little tricky because

typically F refers to um the F2 or F3 it

refers to the whole organism and in

animals and plants that means a diploid

our funi never form a diploid if you

remember we always have like n plus n so

we don't get a diploid organism until we

make that basidium or that bidia plural

uh on the mushroom fruit body so

technically our mushrooms um they're not

technically we can't we shouldn't

actually raise the F number but for a

practical purposes you got to assume

that if you got an F2 Generation you

make spores they've undergone meiosis

the subsequent spores the subsequent

fruit the subsequent cultures the

subsequent um whatever uh can be labeled

F3 it's just a practical thing so often

time when I get spores I'll double label

them so if I take a Spore print I might

say F2 spores for me I know that when I

germinate those they're going to become

the F3 okay so when I take those spores

from the F2 and I put them on a new

plate and I germinate them I write F3

okay that's under the assumption that

you're going to take those for you're

going to grow them out you're going to

make more fruit and then when those

fruit undergo meiosis in the bidia

they're going to make the F4 so at some

point you need to Callum F3 wherever you

want to do that is up to you I generally

prer to um if I had F2 spores if I

germinate them I'm going to label those

plates F3 I'm going to label the the

spawn and the tub F3 and when I make my

new spores I'm going to make that F4 so

this is why a lot of the I hate to get

kind of controversial but this is is why

a lot of the old school um people that

don't want you to do they don't want you

to catch up okay so let's say your

typical mushroom generation goes so

that's another word you might see G I'm

not going to go there uh G for

Generation you'll see that in the

literature um we're not going to use

that term here because it's not really

generally used you may see it in Paul

stam's books uh and some of the older

literature um these days we kind of

because G is a is a a whole other G

usually refers to um spawn so when we do

G2 G grain to grain the G is actually a

reference to Generation Um so if you

have a master spawn jar and you turn

that into 10 jars and then you take one

of those 10 and you expand it again and

you do a G2 g g is grain grain to grain

um the G if you see it on like a plate

or a label it means generation so again

if I had a mass Master jar of Spawn and

I did g2g anime 10 jars that would be

you know the the G2 second generation

again if you want to call maybe you're

calling the master and then you got G1

or G2 however you label it you need to

have subsequent numbers you know

ascending numbers so so you get the idea

there again that this is already getting

way more complicated than I thought it

was going to

be you can write questions down at the

bottom uh I might have to do all of

these in a more um like a own their own

little video and I'm probably up to

about yep 20 minutes already oh my

goodness so you guys um I'm going to

like maybe let me just go I might have

to make other videos you guys these

videos are getting way too long and I

just saw it's at 20 minutes already as

you can tell I can I can talk about

these topics forever and ever and ever

so let's just review real quick here

remember we had t for

transfer uh we had C for clone and we

have f for filial um Ms stands for

multipore I talked about that a little

bit already terms WT WT means wild type

okay so this is again something that

doesn't get used in the uh mological

Community but I think should because

it's a great term um you will see it in

the literature talking about bacteria if

you isolate something from the wild you

call it wild type or WT you little

graphs and stuff where they compare like

say A you know a wild Cube to you know I

don't know Enigma or something like that

um you might see

WT land race you know all the plant

people know that are there Cube Land

races anymore probably not um we have

moved cattle and other ruminants all

around the world and I don't really

think this term land race really applies

to cubes anymore or cannabis for that

matter I like the term Legacy I'm going

to try to start using that term a little

bit more um so something like you know

you would say like oh manami you know

from Japan is a a legacy race something

like Burma or Cambodia or you know a

Koso or something like that um you would

say that that was maybe a legacy St rain

or a legacy cultigen H my little bugs I

never see bugs until I

start I don't know what that one is I

had the door open earlier in my

kitchen started pouring down rain the

first big rain we've had this year so

the bugs are coming out um any who back

multipore land race Legacy um whatever

you know whatever word you want to use

and finally mono carryon and I think I'm

going to end it there you guys

because uh I I just realized I'm going

to have to go into these terms maybe in

way way way more detail but I hope that

starts you guys off um somewhere um the

other thing is going to take like an

hour and a half to upload this to

YouTube it's not like the lives where

they go directly I actually have to um

like upload this to YouTube ah the

resolution is much better obviously and

I hope it hasn't been doing that Focus

thing you guys I know I've been moving

around a lot um anyway if you have

questions put them down uh below and uh

I'll be sure I've got a list I don't

know if you guys I got my other

whiteboard um I've got this this is the

list of

videos that I'm going to make

so there's going to be a lot of videos

like six months ago I was like what what

can I possibly make another video about

um yeah I've got 16 there and another

like 15 pages of ideas on my computer so

I hope you guys like that I hope I

didn't miss anything too glaringly

obvious I probably will uh will notice

that I did when I when I rewatch this um

but any who you guys I hope you all have

a good day and um I will we'll talk to

you later for sure bye-bye