Uji Glukosa - uji menggunakan Fehling A dan Fehling B

Biologi Asyik

30 Nov 202111:26

Summary

TLDRIn this educational video, the host demonstrates a glucose test to determine the sugar content in everyday foods. Using ingredients like carrot juice, lemon, and tomato juice, along with sugar and milk, the process involves mixing these into solutions and applying a glucose indicator solution. After heating the mixtures, the presence of glucose is indicated by a red precipitate at the bottom of the reaction tubes. The experiment concludes with a comparison of the results, revealing which foods contain glucose and which do not, providing viewers with a hands-on method to explore the sugar content in their diets.

Takeaways

🔍 The video is about a glucose test to determine the sugar content in everyday foods.
📝 The required materials for the glucose test include carrots, lemons, tomatoes, sugar, and milk.
🔬 The materials are prepared as solutions: carrot, lemon, and tomato juices, sugar dissolved in water, and milk.
🧪 The equipment used includes a burner, a tripod, a reaction tube with a rack, a glass beaker, a dropper, and a wooden clamp.
🏷 The first step is to label the reaction tubes and place the prepared solutions into them.
🔴 The initial color of each solution is noted before the test, ranging from orange for carrot juice to red for tomato juice.
💧 The test involves heating water to a certain temperature and adding 10 ml of each solution to be tested.
📝 The Fehling solution A and B are used as indicators, with three drops of each added to the solutions.
🔑 After mixing the indicators, the solutions are shaken and the initial color changes are observed.
🔥 The solutions are then heated in a glass beaker with hot water for about 5 minutes to observe any color changes indicating the presence of glucose.
📊 The presence of glucose is confirmed by the formation of a red precipitate at the bottom of the reaction tube.
🍎 Among the tested substances, carrot juice, milk, sugar solution, and tomato juice showed the presence of glucose, while lemon water did not.
👨‍🏫 The video encourages viewers to try the test at home or in the laboratory when they return to school.

Q & A

What is the main purpose of the glucose test described in the script?
-The main purpose of the glucose test is to determine the glucose content in everyday foods.
What are the materials used in the glucose test according to the script?
-The materials used in the glucose test include carrot juice, lemon juice, tomato juice, sugar, and pure milk.
What are the tools required for the glucose test as mentioned in the script?
-The tools required for the glucose test include a burner, a tripod, a reaction tube with a rack, a glass beaker, a dropper for the indicator solution, and a wooden clamp.
How many types of indicator solutions are mentioned in the script for the glucose test?
-Three types of indicator solutions are mentioned: Fehling A, Fehling B, and Benedict solution.
What is the initial color of the carrot juice mentioned in the script?
-The initial color of the carrot juice is orange.
What is the initial color of the milk in the glucose test?
-The initial color of the milk is white.
What is the expected final color change for the glucose test if glucose is present?
-If glucose is present, the expected final color change is a red precipitate at the bottom of the reaction tube.
How long should the reaction tubes be heated in the glucose test?
-The reaction tubes should be heated for approximately 5 minutes.
What does the absence of a precipitate in the lemon water indicate in the glucose test?
-The absence of a precipitate in the lemon water indicates that it does not contain glucose.
Based on the script, which of the tested samples contain glucose?
-Based on the script, the samples that contain glucose are carrot juice, milk, sugar water, and tomato juice.
What is the conclusion of the glucose test as per the script?
-The conclusion of the glucose test is that carrot juice, milk, sugar water, and tomato juice contain glucose, while lemon water does not.

Outlines

00:00

🔬 Introduction and Materials for Glucose Test

The video begins with an introduction to the channel and the objective of the video, which is to test for glucose in everyday food items. The materials needed for the test include carrots, lemons, tomatoes, sugar, and fresh milk, which will be converted into solutions. Various laboratory equipment such as a spirit burner, test tubes, pipettes, and a beaker are also listed. The procedure starts by labeling the test tubes and preparing the solutions for testing.

05:02

🧪 Conducting the Glucose Test

The process of adding the prepared solutions to the test tubes is described, with an initial observation of their colors. Fehling's solution A and B are used as indicators, added to each sample in specific amounts. The samples are mixed and initial color changes are noted. The video emphasizes careful handling of the materials and highlights the importance of observing any color changes as an indication of glucose presence.

10:03

🔥 Heating the Samples and Observing Results

The samples are heated in a beaker with hot water for about five minutes to observe further reactions. After heating, each sample is examined for the presence of a reddish-brown precipitate at the bottom of the test tube, which indicates the presence of glucose. The video details the results for each sample, concluding that carrot juice, milk, sugar solution, and tomato juice contain glucose, while lemon juice does not. The video ends with an encouragement to viewers to try the test themselves at home or in their school labs.

Mindmap

Keywords

💡Glucose Test

The glucose test is the main focus of the video, aimed at detecting the presence of glucose in various foods. The process involves using specific chemical reagents to reveal glucose content, essential for understanding the nutritional value of everyday foods. The video demonstrates this test using carrots, oranges, lemons, tomatoes, sugar, and milk.

💡Fehling's Solution

Fehling's solution is a chemical reagent used in the glucose test to detect the presence of reducing sugars. It consists of Fehling A and Fehling B solutions, which are added to food samples to cause a color change indicating the presence of glucose. The video shows how Fehling's solution is applied to different food samples to observe reactions.

💡Indicator

An indicator in the context of this video is a substance that changes color to signal the presence of another substance. Fehling's solution acts as an indicator for glucose. The video explains how adding Fehling's solution to food samples causes a color change if glucose is present.

💡Food Samples

Food samples refer to the various food items tested for glucose content in the video. These include carrot juice, milk, lemon juice, sugar water, and tomato juice. The video illustrates the preparation of these samples and their subsequent testing with Fehling's solution.

💡Spirit Burner

A spirit burner is a piece of laboratory equipment used to heat substances. In the video, it is used to heat water, which in turn heats the test tubes containing food samples and Fehling's solution, facilitating the glucose test.

💡Color Change

Color change is a key observation in the glucose test, indicating the presence of glucose. In the video, food samples mixed with Fehling's solution change color, such as turning greenish or reddish-brown, showing the presence of glucose.

💡Precipitate

A precipitate is a solid formed in a solution during a chemical reaction. The video mentions the formation of a reddish-brown precipitate at the bottom of the test tubes, indicating the presence of glucose in the tested food samples.

💡Heating Process

The heating process is essential in the glucose test to facilitate the chemical reaction between the food samples and Fehling's solution. The video demonstrates heating the samples in a water bath for about five minutes to observe any color change or precipitate formation.

💡Reducing Sugars

Reducing sugars, like glucose, can donate electrons to another chemical, causing a reduction reaction. Fehling's solution tests for these sugars in food samples. The video shows the presence of reducing sugars in the samples through the observed reactions.

💡Laboratory Equipment

Laboratory equipment refers to the tools and devices used in the glucose test, including test tubes, a spirit burner, a water bath, pipettes, and a test tube holder. The video details the usage of each piece of equipment in conducting the glucose test.

Highlights

Introduction to the glucose test to determine the glucose content in everyday foods.

List of materials used in the glucose test including carrots, lemons, tomatoes, sugar, and milk.

Preparation of the materials in liquid form for the test.

Use of a spirit burner, tripod, and other laboratory equipment for the glucose test.

Labeling of the reaction tubes and placing the liquid materials inside.

Initial observation of the color of each liquid material before the test.

Use of Fehling's solution as an indicator for the presence of glucose.

Adding three drops of Fehling A solution to each sample.

Subsequent addition of three drops of Fehling B solution to each sample.

Observation of color changes in the samples after the addition of Fehling's solution.

Comparison of the initial and post-addition color of the samples.

Heating the samples in a glass beaker with hot water to observe glucose presence.

Expectation of a red precipitate indicating the presence of glucose.

Five-minute heating process to allow for glucose detection.

Analysis of the results after heating, identifying the presence of glucose in certain samples.

Conclusion that carrot juice, milk, sugar water, and tomato juice contain glucose, while lemon water does not.

Encouragement for viewers to try the glucose test themselves or in a school laboratory.