TEKNIK DASAR PEMOTONGAN BLOCK PARAFIN MENGGUNAKAN MICROTOM LEICA RM 2235

Summary

This video tutorial provides a step-by-step guide on how to operate a ROM microtome, a cutting tool used in histopathology to create tissue slides. The presenter walks viewers through setting up the device, positioning the paraffin block, and adjusting the knife for both trimming and finishing cuts. Emphasis is placed on using the right type of knife—blunt for trimming and sharp for precise cutting. The tutorial also highlights safety practices and the cleaning process after use. Aimed at beginners, the video offers helpful tips for mastering microtome operations and improving cutting techniques.

Takeaways

• 😀 The tutorial demonstrates how to use a ROM microtome to cut histopathology tissue slides.
• 😀 The microtome used in the video is the RM 22350 model from the brand 'Promise'.
• 😀 The microtome includes various components such as the knife holder, locking parts, and paraffin block holder.
• 😀 The process begins with setting up the microtome by placing the paraffin block and positioning the knife.
• 😀 To ensure smooth cutting, the knife holder can be adjusted to switch between sharp and blunt knives.
• 😀 Blunt knives are used for trimming tissue surfaces, while sharp knives are used for the finishing cuts.
• 😀 The paraffin block is securely placed and locked in position before starting the cutting process.
• 😀 The cutting procedure involves carefully slicing the tissue to a thin, even layer using the sharp knife.
• 😀 The tissue is stretched after cutting to ensure it is flat and ready for further processing.
• 😀 After the cutting is completed, the knife is cleaned, and the waste is disposed of properly in a medical waste container.
• 😀 The tutorial emphasizes that the presenter is still a beginner, but they aim to improve their skills in using the microtome for more precise and professional results in the future.

Q & A

What is the main purpose of using a microtome in histopathology?

The main purpose of using a microtome in histopathology is to cut tissue samples embedded in paraffin into very thin sections, which are then examined under a microscope for diagnostic purposes.

What model of microtome is being used in the tutorial?

The model of the microtome being used in the tutorial is the Promise RM 22350.

What is the function of the knife holder in the microtome?

The knife holder in the microtome holds the cutting knife securely. It can be adjusted left or right to position either a blunt or sharp knife depending on the stage of the cutting process.

Why is it important to adjust the knife position between blunt and sharp knives?

It is important to adjust the knife position between blunt and sharp knives because a blunt knife is used for trimming the outer paraffin layer, while a sharp knife is used for making the final, precise cuts of the tissue.

How do you position the paraffin block in the microtome?

To position the paraffin block, you place it in the holder, ensuring that it is properly aligned. The holder can be adjusted forward or backward to fine-tune the position of the block.

What thickness setting is recommended for beginners when using the microtome?

For beginners, a tissue thickness of 3 microns is recommended, as it is easier to work with when first learning how to operate the microtome.

What should you do after cutting the tissue with the sharp knife?

After cutting the tissue with the sharp knife, you should gently stretch the tissue to make it flat before placing it on a slide for further processing.

What is the purpose of using a blunt knife during the trimming stage?

The purpose of using a blunt knife during the trimming stage is to remove the outer paraffin layer and expose the tissue for a smoother and more precise cut with a sharp knife in the finishing stage.

Why is it important to lock the paraffin block and knife holder during the cutting process?

It is important to lock both the paraffin block and knife holder during the cutting process to ensure they do not shift, which could disrupt the cutting and result in uneven or inaccurate sections.

What should be done with the waste generated during the cutting process?

The waste generated during the cutting process, such as tissue remnants and paraffin, should be properly disposed of in a medical waste container to prevent contamination and maintain safety in the lab.