pengenalan lab biotek faperta

Summary

This video provides an introduction to various laboratory tools used in agricultural biotechnology. It covers the function and usage of several essential instruments, including the filler (rubber boat) for transferring strong acid and base solutions, the Erlenmeyer flask for media preparation, and the measuring cup for liquid volumes. The video also discusses other tools such as pipettes, tweezers, scissors, scalpels, incubators, analytical balances, water baths, and autoclaves. Additionally, it highlights advanced tools like electrophoresis equipment, spectrophotometers, and laminar air flow workstations used for precise experiments in biotechnology research.

Takeaways

• 😀 Filler or rubber boat is used for sucking strong acid and base solutions with the help of a pipette, allowing controlled release and suction of liquids.
• 😀 Erlenmeyer flask is used as a container for creating media and quantitative measurements in laboratory experiments.
• 😀 Beaker glass serves as a container for aquades, reagents, or media preparation and is used for quantitative measurements.
• 😀 Graduated cylinder is an instrument used to measure the volume of liquids with moderate accuracy.
• 😀 Test tubes are used to hold small amounts of liquids during experiments.
• 😀 Tweezers are designed for gripping and transferring materials in laboratory practices.
• 😀 Scissors are used for cutting explants or other materials for experimentation.
• 😀 Scalpel is used for cutting explants or specimens with precision.
• 😀 Dropper pipette is employed to transfer small amounts of liquid.
• 😀 Stirring rod or glass stirrer is used to mix solutions or reagents during experiments.
• 😀 Acid cabinets are used for safely handling and reacting strong acids and bases, preventing exposure to harmful fumes.
• 😀 Aquabidestilator or water distiller is used to produce distilled water and aquabidest.
• 😀 Oven is utilized for drying or heating laboratory equipment and objects.
• 😀 Analytical balance measures the weight of small quantities of chemical substances with high precision.
• 😀 Water bath is used to heat reagents or incubate cell cultures.
• 😀 Hot plate is used for homogenizing liquid solutions through mechanical stirring and heating.
• 😀 Centrifuge is used to separate solids from liquids by spinning at high speed.
• 😀 Electrophoresis equipment helps analyze the size and shape of particles like DNA, RNA, and proteins.
• 😀 Spectrophotometer is used to measure the absorbance of samples using ultraviolet light.
• 😀 Autoclave is a closed sterilization system that uses high-temperature, high-pressure steam to sterilize equipment and materials.
• 😀 Transilluminator is used to visualize DNA after electrophoresis and to measure concentrations of molecules such as DNA, RNA, and proteins.
• 😀 Laminar air flow is a sterile workbench used for tissue culture procedures.

Q & A

What is the function of the filler or rubber boat in laboratory biotechnology?

The filler or rubber boat is used as a suction device for strong acidic and basic solutions. It is connected to a pipette, with valves to control the suction and release of the solution in the pipette.

How does the filler or rubber boat operate with the pipette?

The rubber boat operates by using a valve marked with 'Aa' to release air and create a bubble, a valve marked with 'Es' to suck the solution into the pipette, and another valve marked 'E-United' to release the solution from the pipette.

What is the purpose of the Erlenmeyer flask in agricultural biotechnology?

The Erlenmeyer flask serves as a container for making media and conducting quantitative measurements in laboratory experiments.

What is the function of a beaker glass in laboratory practices?

The beaker glass is used as a container for aquades, reagents, or for the preparation of media and quantitative measurements.

When is a graduated cylinder used in a laboratory?

A graduated cylinder is used for measuring the volume of liquids or solutions where high precision is not required.

What is the role of test tubes in laboratory experiments?

Test tubes are used as small containers to hold liquids in small quantities during experiments.

What are the uses of tweezers in the laboratory?

Tweezers are used to pinch or pick up materials that will be used in experiments or practical work.

What is the function of scissors in laboratory procedures?

Scissors are used to cut explants or materials that will be used in experiments.

What is a scalpel used for in the laboratory?

A scalpel or blade handle is used to cut explants or samples during laboratory procedures.

What is the purpose of a pipette in laboratory work?

A pipette is used for transferring small volumes of liquid in precise measurements, often used for delicate tasks in biotechnology experiments.

What does a laminar air flow system provide in laboratory conditions?

A laminar air flow system provides a sterile workspace for tissue culture and other biological experiments by filtering out contaminants in the air.

What are the functions of an autoclave in laboratory settings?

An autoclave is used to sterilize equipment and materials by using high-pressure steam. It includes controls for time, temperature, and pressure, and should not be opened while hot to avoid contamination.

How does a spectrophotometer contribute to laboratory analysis?

A spectrophotometer is used to measure the absorbance of a sample by passing ultraviolet light through it. It helps identify the concentration of various substances like DNA, RNA, and proteins.

What is the function of a centrifuge in biotechnology experiments?

A centrifuge is used to separate solid particles or precipitates from liquids by spinning the sample at high speeds.

What is the role of a hot plate in laboratory experiments?

A hot plate is used to heat or homogenize solutions and mixtures through mechanical stirring and heating.

What is the purpose of a water bath in biotechnology?

A water bath is used for heating reagents, incubating cell cultures, or melting biological samples in a controlled temperature environment.