Pollinating mother plant and collecting tissue samples
Summary
TLDRIn this instructional video, viewers learn about plant pollination and identification. The presenter guides through the process of pollinating a mother plant using cotton swabs to transfer pollen from 'father' plants. Key plant parts like cotyledons, nodes, and hypocotyls are explained, with emphasis on their characteristics for identification. The video also covers the importance of recording plant color and taking samples for DNA analysis, setting the stage for a comprehensive lab on plant reproduction.
Takeaways
- 📚 Read lab manual pages 57 to 74 for instructions on plant pollination and sampling.
- 🌿 Learn the parts of a plant, including cotyledons (embryonic leaves) and true leaves (irregular shaped leaves).
- 🔍 Identify the node (where leaves emerge from the stem) and the internode (stem between nodes).
- 🟣 Recognize the hypocotyl (stem part below cotyledons), which is important for plant identification, with different colors like purple or green.
- 🌼 Understand the process of pollination using a cotton swab to transfer pollen from father plants to the mother plant.
- 🧑🔬 Pollinate the mother plant with pollen from both father plants (F1 and F2) without concern for mixing, as it's part of the experiment.
- 📝 Record the color of the hypocotyl for each father plant and the mother plant in the lab manual for later reference.
- 🧊 Store collected samples in the freezer for future DNA purification and analysis.
- 🌱 For father plants, take as many leaves as needed for the experiment, as they will be discarded afterward.
- 🌿 From the mother plant, take a cotyledon and a true leaf for sampling, ensuring not to harm the plant by taking too many leaves.
Q & A
What are the pages in the lab manual that students should read before the demonstration?
-Students should read pages 57 to 74 in the lab manual.
What part of the plant is being discussed on page 70 of the lab manual?
-On page 70, the lab manual discusses the various parts of a flower and plant, including the cotyledon.
What are cotyledons and how can they be recognized?
-Cotyledons are the embryonic leaves that are the first to emerge from a plant. They can be recognized as smooth, rounded leaves that look different from the true leaves, which have an irregular shape.
What is a node and how is it different from an internode?
-A node is the point on a stem where leaves emerge. An internode, on the other hand, is the section of the stem that lies between two nodes.
What is the hypocotyl and why is its color important?
-The hypocotyl is the part of the stem that is below the cotyledons. Its color is important because it can be used to identify different plant varieties.
How does one pollinate the mother plant in the demonstration?
-To pollinate the mother plant, one uses a cotton swab to roll over the flowers of the father plant and then transfers it to the flowers on the mother plant, transferring pollen in the process.
What is the significance of having both a father one and father two in the demonstration?
-Having both father one and father two allows for the chance of either pollen type to pollinate the mother plant, and it will be part of the experiment to determine which father plant's pollen is successful.
What should be recorded for each plant after pollination?
-After pollination, the color of the hypocotyl (non-purple for father one and purple for father two), the ID (F1 for father one and F2 for father two), and the section number should be recorded.
Why are leaves from the father plants discarded after sampling?
-Leaves from the father plants are discarded after sampling because they are no longer needed for the experiment and will be analyzed separately.
What parts of the mother plant are taken for the experiment?
-For the mother plant, a cotyledon and a true leaf are taken for the experiment, leaving the rest of the plant intact.
What will be done with the collected samples from the plants?
-The collected samples will be stored in the freezer for later analysis, including DNA purification.
Outlines
🌿 Plant Pollination and Identification
This paragraph introduces the process of plant pollination and the importance of identifying plant parts. The speaker instructs viewers to read their lab manual, specifically pages 57 to 74, and to familiarize themselves with plant parts such as cotyledons, nodes, internodes, and hypocotyls. The speaker explains that cotyledons are the first leaves to emerge and are smooth and rounded, while true leaves have an irregular shape. The node is the point where leaves emerge from the stem, and the internode is the stem section between nodes. The hypocotyl is the stem part below the cotyledons, and its color is crucial for plant identification. The speaker demonstrates how to pollinate a mother plant using a cotton swab to transfer pollen from the father plants (referred to as 'father 1' and 'father 2') to the mother plant. The process involves rolling the swab over the flowers of the father plants and then over the mother's flowers, mixing pollen from both fathers. The speaker also emphasizes the importance of recording the color of the hypocotyls for each plant (non-purple for father 1 and purple for father 2) and the mother plant, which is non-purple. Samples are taken and marked for later analysis, and the father plants' leaves are clipped and discarded after use.
🧪 Sample Collection and Storage
The second paragraph focuses on the collection and storage of plant samples for DNA analysis. The speaker demonstrates how to take samples from the mother and father plants, ensuring not to remove too many leaves from the mother plant. Specific plant parts are chosen for sampling, including a cotyledon and a true leaf from the mother plant, and leaves from the father plants. The samples are placed in appropriately marked microcentrifuge tubes, which are then stored in a freezer for future DNA purification and analysis. The speaker also mentions that the leaves from the father plants can be discarded after sampling, as they will not be used again.
Mindmap
Keywords
💡Lab Manual
💡Pollination
💡Cotyledon
💡Node
💡Internode
💡Hypocotyl
💡Father Plant
💡Mother Plant
💡Pistil
💡Stamen
💡DNA Purification
Highlights
Instructed to read lab manual pages 57 to 74 for understanding the process.
Emphasis on familiarizing with flower and plant parts as listed on page 70 of the lab manual.
Definition and identification of cotyledons as the embryonic leaves that emerge first.
Explanation of the difference between cotyledons and true leaves in terms of appearance.
Introduction to the concept of nodes and internodes on a plant stem.
Importance of recognizing the hypocotyl for plant identification and its color variation.
Description of the hypocotyl's location on the plant and its role in plant structure.
Instruction on how to pollinate a mother plant using a cotton swab on father plants.
Process of transferring pollen from father to mother plants without concern for order.
Documentation of plant samples with identification numbers and section numbers.
Differentiation between father plants based on the color of their hypocotyls.
Instructions for collecting and storing samples from mother and father plants for DNA analysis.
Details on how to handle and clip leaves from father plants for sample collection.
Procedure for taking leaves and cotyledons from the mother plant for the experiment.
Mention of future DNA purification and analysis from the collected plant samples.
Transcripts
all right so be sure to read
your lab manual pages 57
to 74
and what i'm going to do is show you
how we pollinate
the mother plant here and take
samples before we do that you should go
over the
list of flower parts
plant parts here let's see it's on page
70 of your lab manual
you want to know what the cotyledon is
the cotyledon
are the embryonic leaves the first
leaves that come out
and you can recognize those because they
look a little different from
the true leaf which are all the other
leaves that are up here that have
kind of an irregular shape whereas the
cotyledons you can see are kind of
smooth
rounded leaves and they're the very
first ones
to to emerge
we also have the inter node which is
well first i should probably show you
the node the node is every place where
leaves
come out from the stem that's a node
this is a node
and so on and the internode
of course would be the stem between the
nodes
then we have the hypocotyl this is an
important one because you need to
recognize
the color of the hypocotyl in order to
identify these plants or one of the
identifications for the plants
the hypocotyl is the part of the stem
that's below
the cotyledons and so it's this part
right here and you can see
on this one which we're going to call
father 2
it's a purple color
on the mother the hypocotyl is green
as is on this other uh father two or
father one sorry that we'll call father
one
um so be sure you know how to identify
those parts
don't worry right now about the pistol
the
stigma stamen anther flower bud and
and ovary you're going to learn about
that
later when we do our experiment on
plant reproduction
so the first thing we want to do is to
pollinate the mother plant the way we do
that is we take a
cotton swab and roll it
over the flower of the father this is
going to be father one
and then roll it over the flowers on the
mother that transfers the pollen
from the father to the mother we're
gonna do
the same thing with father two it
doesn't matter that i'm mixing them up
here
it's merely chance which of these
fathers are going to end up
pollinating the mother plant and so
we'll
try to determine that later when we do
the
rest of the paternity lab
so for each of these
plants the father 1
is going to be
green or in other words non-purple
so we'll put down non-purple
for f1 and you can put as the id
f1 and then your section number in this
case i put 0 2
0 that section number and we check it
off that we've taken the sample
and then for father two uh it's going to
be
purple so we put purple
as the color of the hypocotyl and
we've checked that off i've already done
one sample so that's why these are
already in here um and then the mother
again so f2 for the father two and then
m
for the mother and then whatever section
number you're in
and you're going to put non-purple all
the mothers are going to be non-purple
and we're going to check that off
because we're going to take samples from
each now
and put those in the freezer for later
analysis
so on the father plants you can
clip off as many leaves as you want to
because we're simply going to throw them
away
we're done with them after this
and put them in
a vial that's marked appropriately this
is f2
this is the mother and this is the f1
for
our father plant so we put that
in here
close it up and this will be
put in the freezer for later analysis
we'll be
purifying the dna at a later time and
we'll do the same thing for each one
here the mother plant
we don't want to take too many leaves
from her i'll take a hypo
i'll take a cotyledon and a
a true leaf
and leave the rest there
so this is the mother
and then again for the um f2
the father two we'll take some leaves
and put them
in this last microcentrifuge tube for
later use
okay i think that's it
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