Parkinson's Research Model in Zebrafish

Summary

This video delves into Parkinson's disease, exploring its progression and impact through a zebrafish animal model. It discusses the disease's genetic and environmental factors, dopamine deficiency, and the use of MPTP to induce phenotypic changes. The script covers behavioral tests to observe symptoms like tremors, muscular rigidity, and anxiety, highlighting the model's significance in Parkinson's research and potential for therapeutic development.

Takeaways

• 🧠 Parkinson's disease is the second most common neurodegenerative disorder, characterized by the degeneration of the substantia nigra and dopamine deficiency.
• 🔍 The etiology of Parkinson's is not fully understood but involves genetic, environmental, and aging factors, along with toxins.
• 🐟 The zebrafish animal model is used to study Parkinson's disease, with phenotypic changes induced by MPTP leading to dopamine neuron damage.
• 🧬 Genetic changes in the zebrafish model include alterations in the expression of alpha-synuclein isoforms, which may compensate for motor impairments.
• 💊 The model exhibits both motor and non-motor symptoms, such as tremors, muscular rigidity, and anxiety, which progress with the disease.
• 📊 Behavioral tests in the zebrafish model, like the novel tank test, can indicate anxiety and other stress responses associated with Parkinson's.
• 🔬 Post-mortem analysis of the fish brain can reveal the presence of degenerative cells and Lewy bodies, typical of Parkinson's pathology.
• 🚶‍♂️ Postural instability and gait disturbances are observed in the model, reflecting advanced Parkinson's symptoms in humans.
• 🔄 Stereotyped movements, repetitive behaviors, are indicative of the motor impairments seen in Parkinson's disease.
• 🤖 The zebrafish model is valuable for screening potential treatments and understanding the progression of Parkinson's disease.
• 🌟 Advancements in the zebrafish model with both phenotypic and genotypic changes offer promising avenues for translational research in Parkinson's.

Q & A

What is the main topic discussed in the video script?

The main topic discussed is Parkinson's disease, particularly focusing on phenotypic changes in a zebrafish animal model of the disease.

What is Parkinson's disease?

Parkinson's disease is a slowly progressive neurodegenerative disease associated with the degeneration of the basal ganglia of the brain and a deficiency of the neurotransmitter dopamine. It is the second most common neurodegenerative disease after Alzheimer's.

What are some factors that contribute to the etiology of Parkinson's disease?

Factors contributing to the etiology of Parkinson's disease include genetic mutations, environmental triggers, aging, and toxins.

What animal model is used in the study of Parkinson's disease in this script?

The animal model used is the zebrafish.

What compound is used to induce Parkinson's-like symptoms in the zebrafish model?

The compound used to induce Parkinson's-like symptoms in zebrafish is MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine).

What are some phenotypic changes observed in zebrafish with Parkinson's disease?

Phenotypic changes observed include abnormalities in body posture, tremor, muscular rigidity, movement loss, and anxiety.

How is tremor assessed in the zebrafish model?

Tremor is assessed by introducing the zebrafish into a small tank and observing the number of tremors, particularly in the tail region, in response to a given time.

What method is used to evaluate muscular rigidity in zebrafish?

Muscular rigidity is evaluated by introducing the fish into an experimental tank and counting the number of times the fish bends in response to a touch stimulus using a blunt capillary tube.

What behavioral assay is used to assess anxiety in zebrafish with Parkinson's disease?

The novel tank diving test is used to assess anxiety. This test measures the time spent by the fish in the dark zone versus exploring the tank, with more time in the dark zone indicating anxiety.

What changes are observed in the brain histology of zebrafish with Parkinson's disease?

The brain histology of zebrafish with Parkinson's disease shows aggregates of degenerative cells and abnormal cell development compared to normal cells in the control fish.

What is postural instability, and how is it observed in the zebrafish model?

Postural instability is the loss of postural reflexes, leading to balance issues. In the zebrafish model, it is observed by recording the fish's body stability during both static and dynamic tasks.

What is the significance of stereotyped movement in Parkinson's disease zebrafish model?

Stereotyped movement, which is repetitive and purposeless, is a significant symptom indicating behavioral changes in the zebrafish model of Parkinson's disease.

What is bradykinesia, and how is it studied in the zebrafish model?

Bradykinesia refers to the slowness of movement, a hallmark of Parkinson's disease. It is studied by counting the number of quadrant changes the zebrafish makes in a divided tank within a given period.

Outlines

00:00

🧬 Parkinson's Disease and Zebrafish Animal Model

This paragraph introduces the topic of Parkinson's disease, a progressive neurodegenerative disorder characterized by the degeneration of the substantia nigra and dopamine deficiency. The zebrafish model is highlighted as a significant tool in studying the disease due to its phenotypic and genotypic changes post-MPTP induction, which mimic the human condition. The paragraph discusses the role of alpha-synuclein and the impact on the dopaminergic system, leading to motor impairments in zebrafish. It also outlines various behavioral tests used to assess Parkinson's disease symptoms in the model, including resting tremors, muscular rigidity, and uncoordinated movement, which are indicative of the disease's progression.

05:00

🔍 Behavioral and Neurological Assessments in Parkinson's Disease Models

The second paragraph delves into specific behavioral and neurological assessments used to evaluate the effects of Parkinson's disease in zebrafish models. It describes tests for anxiety, motor coordination, and postural stability, which are crucial for understanding the disease's impact. The paragraph explains how these tests, including the novel tank test and the assessment of stereotyped movements, help in identifying anxiety-like behavior and motor impairments associated with Parkinson's. It also touches on the use of brain cytology to observe degenerative cell changes and the importance of these models in advancing translational research for Parkinson's disease.

Keywords

💡Parkinson's disease

Parkinson's disease is a slowly progressive neurodegenerative disorder, second in prevalence only to Alzheimer's disease. It is characterized by the degeneration of the basal ganglia in the brain and a deficiency in the neurotransmitter dopamine. The video discusses its symptoms and how it is modeled in zebrafish to study phenotypic changes.

💡phenotypic changes

Phenotypic changes refer to observable traits or characteristics that change due to underlying genetic or environmental influences. In the video, these changes are discussed in the context of zebrafish models exhibiting symptoms of Parkinson's disease, such as motor impairments and anxiety.

💡zebrafish animal model

A zebrafish animal model is used in scientific research to study diseases and biological processes due to its genetic similarity to humans. The video focuses on how zebrafish are used to model Parkinson's disease, showing similar motor and behavioral symptoms as humans.

💡dopaminergic neurons

Dopaminergic neurons are nerve cells that produce the neurotransmitter dopamine, essential for regulating movement and coordination. The video explains how the destruction of these neurons leads to Parkinson's disease symptoms in both humans and zebrafish models.

💡MPTP

MPTP is a neurotoxin used to induce Parkinson's disease in animal models by destroying dopaminergic neurons. The video describes how MPTP is used to simulate Parkinson's in zebrafish, leading to similar neurodegenerative effects observed in humans.

💡motor symptoms

Motor symptoms in Parkinson's disease include tremors, muscular rigidity, and movement loss. The video illustrates how these symptoms are replicated in zebrafish models to study the disease's progression and potential treatments.

💡alpha-synuclein

Alpha-synuclein is a protein implicated in the development of Parkinson's disease, with its abnormal accumulation leading to neurodegeneration. The video discusses how different isoforms of alpha-synuclein are expressed in zebrafish to study their role in Parkinson's.

💡genetic mutation

Genetic mutations are alterations in DNA that can contribute to the development of diseases, including Parkinson's. The video mentions genetic mutations as one of the factors influencing the onset of Parkinson's disease, along with environmental triggers and aging.

💡resting tremor

A resting tremor is an involuntary shaking movement that occurs when muscles are relaxed, commonly associated with Parkinson's disease. The video shows how resting tremors are observed in zebrafish models, particularly in the tail region, mimicking human symptoms.

💡anxiety

Anxiety is a non-motor symptom of Parkinson's disease, characterized by feelings of worry and nervousness. The video describes how zebrafish models exhibit anxiety-like behaviors, such as spending more time in the dark zones of a tank, to study this aspect of the disease.

Highlights

Introduction to Parkinson's disease and its significance as the second most common neurodegenerative disease.

Parkinson's disease is associated with the degeneration of the basal ganglia of the brain and deficiency of the neurotransmitter dopamine.

Etiology of Parkinson's disease remains unknown, but factors include genetic mutation, environmental triggers, aging, and toxins.

Use of zebrafish as an animal model to study phenotypic changes related to Parkinson's disease.

MPTP used to induce Parkinson's disease in zebrafish, leading to the generation of reactive oxygen species.

Destruction of dopaminergic cells is a key aspect of Parkinson's disease progression.

Humans with Parkinson's disease exhibit motor and non-motor symptoms.

Zebrafish model exhibits both phenotypic and genotypic changes post-induction.

Alpha-synuclein plays a significant role in genetic changes observed in Parkinson's models.

Zebrafish alpha-synuclein is closest to human alpha-synuclein in function.

Motor impairments in zebrafish become more severe with the expression of alpha-synuclein.

Phenotypic changes in zebrafish include abnormalities in body posture, tremor, muscular rigidity, movement loss, and anxiety.

Experimental setups are used to validate the prognosis of Parkinson's disease in response to behavioral changes in zebrafish.

Resting tremor and action tremor are two types of tremors associated with Parkinson's disease.

Behavioral assays in zebrafish include response to hectic stimuli, jaw movement recording, and novel tank diving tests.

Transcripts

hi everyone this is your metal duck from

Metacritic I'm very happy to see you

back again now with our new topic in

position the topic for this issue is

based on Parkinson's disease up next our

position the topic for this session is

with a Parkinson's exhibit phenotypic

changes in zebrafish animal model

Parkinson's disease is a slowly

progressive second most common

neurodegenerative disease after as cheap

as it is associated with the

regeneration of the Bassel Campea of the

brain and the deficiency of the

neurotransmitter dopamine as a consensus

acrimony can progress if this is the

degree of disability varies among the

effector projects it is also known as

paralyzes residents unchanging path see

the etiology of Parkinson's remains

unknown but several factors play a role

including genetic mutation environmental

triggers aging and toxins of phenotype

changes during Parkinson's zip lavash

were in used with the MPTP after

induction the mood of our main oxidase

be thus taken up by other dopamine

transporter into dopamine neurons

whether it leads to the increased

generation of reactive oxygen species

mostly young BTK effects enter

dopaminergic system the progression of

Parkinson's disease occurs due to the

destruction of dopaminergic cells next

is modern development in Parkinson's

disease

humans with Parkinson's disease inhibit

motor symptoms as well as non-motor

symptoms signal fish animal model with

Parkinson's disease processes

motor symptoms comparatively progress

depends of human symptoms of post

induction the model starts exhibits both

phenotype and genotype changes any

abilities or changes in the function of

XX EEG that is simply alpha causes

genetic changes in Parkinson's model

signals which express threesome you

clean isoforms of beta gamma 1 and gamma

2 significance to compensate the axons

of alpha-synuclein functionally the

zebrafish alpha once a new clean appears

to be closest to the human signaling not

Amna of the beta I am gamma 1 see

nucleus in users motor impairments in

zebrafish

which are even more severe when the

expression of Watson you cleaners after

they have the tracking works and

nutrients have an abnormal development

of the dopaminergic system including

delayed differentiation of dopaminergic

neurons and reduced levels of the common

which results in Parkinson's disease

phenotype exchangers include the

abnormalities in the body posture tremor

muscular rigidity movement loss and

anxiety which are observed with the

progression of the disease model here I

am going to take you through the

phenotype it changes in zebrafish animal

border with packing since this is

different experimental setups are used

to validate the prognosis of Parkinson's

disease in response to the behavioral

changes in the model some of that this

represents the prognosis of Parkinson's

disease with respect to the phenotype of

the asli's are interesting Jemma number

of severe in response to a tech

stimulate a muscular rigidity number of

for job access or uncoordinated movement

died contest psychology of the brain

host of instability stereotype movement

and radical miss yeah first is trauma

ademir is not involuntary quivering

movement or shape or uncontrolled muscle

contraction which manifest as shaking in

body parts there are two types of

tremors occurs in Parkinson's disease

resting tremor and action/drama

the resting tremor is more commonly

associated with Parkinson's disease but

there are action tremor is less common

in some cases there is a probability of

internet drama which is not noticeable

to others in this as we observe the

Equality Movement in the zebrafish the

model is introduced into the small

oxalate attack and observed for number

of temes at a given time the trauma

mostly occurs in the tail region in a

cadenza crayfish on the screen is the

graph between control and water in

response to trauma we observe resting

tremor occurs in the model with

Parkinson's disease next is number of

ribbon in response to hectic stimuli or

muscular rigidity

cement and not which the fish body

follows the curves to form a thin shape

and then the fish propels itself a bit

at an angle from its previous position

using your fastest book using the text

stimulus the fish pond cement in order

to exhibit escape response the fish is

introduced into the small experimental

tank from the housing

and the head was gently touch using no

bland capillary tube the number of see

been in response to a touchback counter

on the screen is the graph between

control and water hundred show sleepin

in response over each touch that the

model shows this response of wind

compared to control this indicated the

development of muscular rigidity which

one of the primary symptoms in

Parkinson's

next is behavioral passes on Chama

mentor Parkinson's disease often leads

to involuntary under the pincer

movements of uncoordinated movement may

be the sign of the starter motor

response that causes an inability to

coordinate vector the study which was

introduced into us more on experimental

time and the jaw movements were recorded

for a period of 10 minutes the number of

job causes was countered from the video

recorded on the screen is the graph

between control and model the motor

shows more behavioral processes on John

come back to control indicating rigidity

of fine verses and in warranty passes

next is nor by die contest the novel

type time test extracts the stress

response that is exactly known you know

regenerative response in normal die tank

the tank is filled with water and

separated into two zones that is drops

of high water surface the night spent in

the upper portion of the gun latency to

reach the upper portion of the tank

number of transitions into the upper

portion and number of erratic humans for

motor using them all by that cactus

the fish is introduced into the study

tank from the housing pack and calculate

the time spent by the fish in the water

and dogs one of the tank up on the

screen is the graph between control and

model the control spends little time in

the dark zone and starts to explore the

tank as a natural behavior whereas the

murder

in contrast prefer to spend more time on

the dark zone of the time indicating the

presence of anxiety makes the psychology

of the brain the elevated level of

factors in the brain may be an early

sign of Parkinson's disease the brain

cytologists now started out in response

to find the nature of cells in water the

fish was euthanized at 4 degrees celsius

and the brain was dissected and fixed on

plastics

net oxidant and yoson stains values -

for differential staining on the screen

is the path

of the brain factor 8 and modern

representing the Uranus's under shows

normal cells where the water shoots

aggregates of degenerative cells and

also the presence of an in-between

develop fat cells

next is postural instability boostin

instability is one of the cardinal

manifestations of Parkinson's disease

you do the laws of hoster reflexes

although it is not common in the early

stages it becomes a clinical concern in

the middle later stages of Parkinson's

disease models with the Parkinson's

disease Humpty please do stability

during both static and dynamic tasks

this assay is performed to observe the

presence or absence of question

instability the fissures introduced into

the experiment attack from the housing

using the neck the unstable portion of

the fish is recorded in a video on the

screen is the video which is represents

the instability in the body portion of

the model and graph represents the

presence of posters and stability model

and access control next stereotyping by

data

stereotyped movement is the repetitive

purposeless movement it is one of the

oppressive or repeated movement symptoms

in Arkansas border occurs in at one

stage the fissures introduced into the

experimental dance from the housing from

the number of stereotyped movement

occurs in a fish for a minute after the

adaptation period of one minute of the

time on the screen is the graph shows

number of stereotyped movement between

hunter and model model shows to detect a

pattern of behavior which is one of the

phenotype biomarkers

next is very famous love daddy Venezia

is known as the movement and as one of

the cardinal manifestations of

Parkinson's diseases it is associated

with that impaired ability to access the

body's position to study the data pain

is not the experimental fishtank was

divided into four quadrants with solid

lines and calculate the number of

quadrant changes for a given period of

time the study fish was introduced into

the experimental tank from the housing

can you see the delta home the number of

quadrants changed by the fish for three

minutes so on the screen is the graph

between control and model representing

the average quadrant change is decreased

in the model in comparison to the

control this indicates of restricted

movement of the modern in comparison to

Parkinson's disease represents the most

frequent hypokinetic syndromes

these include Hakeem Islam gladion is

not gait and balance disturbances

phasing phenomenon and acidity it is

concluded that the market of Parkinson's

phenotypes or observing a zebrafish

animal oil but Parkinson's discusses the

signal which model with phenotype

changes and observed in Parkinson's is

variegated and is widely used in

screening we paint the target index of

translational research is quite

promising with advancement in the model

with both phenotype angelo exchanges

thank you this is metal designing from

you and see you back again with next

talk

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