CarboFerm™ I, rapid carbohydrate test for Neisseria and Moraxella

Summary

This video introduces Carbo Firm, a rapid and reliable method for identifying Neisseria and Moraxella isolates, including Neisseria gonorrhoeae and Moraxella catarrhalis. Utilizing traditional carbohydrate fermentation methods, Carbo Firm provides results in just four hours. The easy procedure involves inoculating test strips with a bacterial culture and incubating them to observe color changes, indicating positive or negative results. The video emphasizes the simplicity of inoculation, the quick turnaround time for results, and the importance of consulting the technical insert for accurate identification, making it an essential tool for laboratories.

Takeaways

• 😀 The Carbo Firm test kit is a reliable method for identifying Neisseria and Moraxella isolates.
• 🕒 Results can be obtained in approximately four hours, making it a rapid testing option.
• 🔬 The kit identifies various Neisseria species, including *Neisseria gonorrhoeae*, *Neisseria meningitidis*, and *Neisseria lactamica*.
• 💧 The test utilizes traditional carbohydrate fermentation methods for accurate identification.
• 🔁 A sterile loop is used to transfer a heavy suspension of a 24-hour culture to the inoculation buffer tube.
• 🌡️ Incubation should be done in an aerobic incubator; do not use a CO2 incubator.
• 🔵 A blue coloration in the butyrate well (row H) indicates a positive result for butyrate.
• 🌈 A color change from red to orange or yellow in the carbohydrate wells indicates positive fermentation.
• 📊 Neisseria species will show negative results for butyrate, while Moraxella catarrhalis will be positive for butyrate.
• 📞 For more information or to place an order, contact Hardy Diagnostics via phone or email.

Q & A

What is Carbo Firm used for?

Carbo Firm is a rapid method for identifying Neisseria species, including *Neisseria gonorrhoeae* and *Neisseria meningitidis*, as well as *Moraxella catarrhalis*.

How long does the Carbo Firm test take to produce results?

The Carbo Firm test takes about four hours to complete.

What types of tests are included in the Carbo Firm procedure?

The Carbo Firm test includes a control and tests for glucose, maltose, lactose, sucrose, and a butyrate test.

What is the first step in the Carbo Firm test procedure?

The first step is to remove the caps from the test strips and place them in the base.

What is the purpose of matching turbidity to a McFarland standard?

Matching the turbidity to a McFarland standard ensures a consistent concentration of the organism for accurate testing.

What should you do with wells two and seven during the test?

You should leave wells two and seven empty.

What are the incubation conditions for the Carbo Firm strips?

The strips should be incubated uncovered in an aerobic incubator and should not be placed in a CO₂ incubator.

How do you interpret a blue coloration in well H?

A blue coloration in well H indicates a positive result for butyrate.

What color change indicates a positive fermentation result in the remaining wells?

A color change from red to orange or yellow indicates a positive fermentation of carbohydrates.

What does the negative control well (well A) indicate?

Well A serves as a negative control and should always remain red, indicating no fermentation has occurred.