Embryo Transfer: Beef Part 2
Summary
TLDRThis script outlines the process of embryo transfer in cattle, highlighting its advantages for rapid offspring production, genetic merit enhancement, and economic benefits. It details the steps from superovulation and artificial insemination to embryo collection, evaluation, freezing, thawing, and transfer into recipient cows. The procedure involves careful handling to ensure sterility and requires recipient cows to be healthy and fertile. Embryo transfer is presented as a valuable tool for progressive cattle breeders to improve herd performance.
Takeaways
- πΌ Embryo transfer is a technique used to increase the production of offspring from valuable females rapidly.
- 𧬠It allows for the sufficient number of calves for accurate progeny testing of females and helps in the propagation of rare breeds.
- π° The process is economically beneficial for exporting animals and increasing the value of the herd through offspring from genetically superior parents.
- π The donor cow and sire must have high genetic merit to ensure the offspring's value to the herd.
- π Recipient cows, while not genetically affecting the calves, should be healthy, fertile, and have good milking ability to contribute to the calf's weaning weights.
- π The donor cow is superovulated using synchronized injections of follicle-stimulating hormone during standing estrus and is artificially inseminated.
- π§ After collection, embryos are evaluated for viability and then frozen in liquid nitrogen for storage or shipping.
- β± The thawing process involves a four-step procedure to rehydrate the frozen embryos before transfer.
- π The transfer process requires careful handling to prevent contamination and ensure the embryo is correctly placed within the recipient.
- π οΈ An epidural anesthesia is used on the recipient to relax the reproductive tract and prevent rectal contractions during the transfer.
- π The transfer gun is used to insert the embryo into the uterine horn of the recipient, a process that should be rapid and precise.
- π Embryo transfer is a valuable procedure for progressive cattle breeders looking to enhance the performance and profitability of their herds.
Q & A
What is the purpose of the third well in the embryo transfer process described in the script?
-The third well is used for a final wash of the embryo, containing only sucrose, before it is loaded into the straw for transfer.
How long is the embryo left in the third well before moving to the fourth well?
-The embryo is left in the third well for an additional 6 minutes as per the timer set for the process.
What is the role of the qu CC straw in the embryo transfer process?
-The qu CC straw is used to load the embryo for transfer. It needs to be identified with the particular embryo being transferred and is attached to a 1 cc tuberculin syringe for the procedure.
How is the embryo drawn into the straw during the transfer process?
-The embryo is drawn into the straw by creating a series of air pockets and bands of fluid, which are pulled through the straw after the tip is placed into the solution.
What is the purpose of the cotton plug in the straw during the embryo transfer process?
-The cotton plug in the straw is activated by drawing up the solution, which then seals the straw, making it ready for transfer.
Why is an epidural anesthesia used for the recipient in the embryo transfer process?
-Epidural anesthesia is used to prevent rectal contractions, relax the reproductive tract, and ensure a smooth transfer process for the recipient.
How much anane solution is typically used for epidural anesthesia per recipient?
-Approximately 5 cc's of the 2% anane solution is used per recipient for the epidural anesthesia.
What is the significance of the plastic shames during the transfer gun insertion in the recipient?
-The plastic shames helps to break when the transfer gun touches the cervix, allowing the gun to be gently worked through the cervix without the need to spread the vulva.
How far into the uterine horn should the transfer gun be inserted during the embryo transfer?
-The transfer gun should be slid approximately 3 to 4 inches into the uterine horn to ensure proper placement of the embryo.
What are some advantages of embryo transfer mentioned in the script?
-Advantages of embryo transfer include rapid production of offspring from valuable females, sufficient numbers for accurate progeny testing, propagation of rare breeds, and a more economical way to export animals.
What are the criteria for selecting donor and recipient cows for embryo transfer?
-The donor cow and sire must have high genetic merit, while the recipient cows must be sound, fertile, have good dispositions, and good milking ability to contribute to the calf's weaning weights.
What is the process for preparing frozen embryos for transfer as described in the script?
-Frozen embryos are thawed and rehydrated in a four-step process before being transferred to the recipient females for gestation and parturition.
Outlines
π Embryo Transfer Process and Preparation
This paragraph outlines the detailed steps of the embryo transfer process in cattle. It begins with the preparation of the embryo in well number three with sucrose and a six-minute timer. The embryo is then moved to well number four for the final wash before being loaded into a quarantine cc straw. The straw is prepared by attaching it to a 1 cc tuberculin syringe, drawing up the solution, and sealing it with a cotton plug. The paragraph also covers the use of epidural anesthesia for the recipient cow to relax the reproductive tract and prevent contamination during the transfer. The transfer itself involves inserting the gun through the vulva and cervix into the uterine horn to deposit the embryo. The importance of selecting genetically superior donor and recipient cows is emphasized, as well as the process of superovulation and artificial insemination of the donor cow.
π Benefits and Considerations of Embryo Transfer
The second paragraph focuses on the benefits of embryo transfer in cattle breeding, such as rapid production of offspring from valuable females, sufficient numbers for progeny testing, propagation of rare breeds, and economical export of animals. It also discusses the advantages of this procedure for progressive cattle breeders, encouraging them to consider it as part of new management practices to enhance herd performance. The paragraph concludes with a brief mention of the thawing and rehydration process for frozen embryos before they are transferred to recipient females for gestation and parturition.
Mindmap
Keywords
π‘Embryo Transfer
π‘Glycerol
π‘Sucrose
π‘Timer
π‘Straw
π‘Tuberculin Syringe
π‘Epidural Anesthesia
π‘Superovulation
π‘Artificial Insemination
π‘Liquid Nitrogen
π‘Thawing Process
π‘Progyny Testing
π‘Sterile Bag
Highlights
The process involves transferring embryos from well number three to well number four for a final wash before transfer.
Embryos are loaded into a qu CC straw, identified with the particular embryo to be transferred.
The straw is attached to a 1 cc tuberculin syringe and filled with a specific sequence of fluid bands for embryo transfer.
A cotton plug is activated to seal the straw, preparing it for sterile storage and transfer.
Recipients require epidural anesthesia to relax the reproductive tract and prevent rectal contractions during transfer.
Approximately 5 cc's of anane solution is used per recipient for the epidural anesthesia.
The vulva and perineal region must be cleaned before inserting the transfer gun.
The transfer gun is inserted through the vulva without spreading it, due to the use of a shames.
The transfer gun is gently worked through the cervix and into the uterine horn for embryo placement.
Embryo transfer is rapid, taking only a couple of minutes per animal.
Embryo transfer has advantages such as rapid production of offspring, progyny testing, and propagation of rare breeds.
It provides an economical way to export animals and increase the value of the herd.
Donor cows and sires must have high genetic merit to ensure offspring add value to the herd.
Recipient cows must be sound, fertile, and have good milking ability to contribute to calf weaning weights.
Donor cows are superovulated using synchronized injections of follicle stimulating hormone.
Artificial insemination is performed with two straws of semen 7 days after estrus.
Embryos are evaluated for viability and then frozen in liquid nitrogen for storage or shipping.
A four-step thawing process is used before transferring frozen embryos to recipient females.
Embryo transfer is a useful procedure to increase performance and profitability in superior cattle breeding.
Progressive cattle breeders should consider embryo transfer as a management practice to enhance herd performance.
Transcripts
third well now contains no glycerol only
the
sucrose the timer is set once again for
an additional 6
minutes once the timer goes off the
final time we need to move the embryo
from well number three into well number
four number four is the final wash where
the embryo would then be loaded into the
straw for transfer
the transfer procedure we use a quar cc
straw the straw needs to be identified
with the particular embryo that we're
going to transfer
today the qu CC straw is then attached
onto the 1 cc tuberculin syringe at the
and plug end the tip of the straw is
placed into the solution and a small
amount is drawn up into the syringe we
then take a small air pocket reach down
into the solution and draw the embryo in
a larger band of fluid then another
small air pocket and a very small band
of fluid on the very
end once we have this complete the
solution is drawn up until it activates
the cotton plug and seals the straw now
the straw is ready for transfer
we can place it back in the sterile bag
for
storage the cervix itself so as we
transfer through the vulva and the
vagina this will keep us from
contaminating the sterile portion of the
gun for the recipient we need to use an
epidural
anesthesia this is an anane injection of
2%
we will use approximately 5 cc's per
recipient this will act to prevent
rectal contractions and relaxes the
reproductive
tract each recipient also needs to
receive the epidural
anesthesia once again we will give five
cc's of the anane solution
the epidural takes effect quite
rapidly the vulva and perennial region
is then washed off wiped with a paper
towel and the transfer gun is inserted
through the Volva since you're using a
shames it is not necessary to spread the
vulva prior to entering with the
transfer
gun once the transfer gun touches the
cervix the plastic shames is broken the
transfer gun is pushed through
it gently worked through the
cervix once through the cervix the gun
is slid up into the uterine
horn we normally want to slide it
approximately 3 to 4 in into the uterine
horn once in the uterine horn the embryo
is gently pushed into the
horn the assistant then correctly
identifies the recipient with the embryo
that it
received the transfer process itself is
very rapid and should only take a couple
minutes per
animal now let's review what we have
learned Embryo transfer has several
advantages these include rapid
production of large numbers of Offspring
from valuable females
sufficient numbers of calvs for accurate
progyny testing of
females propagation of rare breeds and
provides a more economical way to export
animals the donor cow and sire must have
high genetic Merit their offspring must
add to the value of the
herd even though the recipient cows do
not have any genetic effect on the
calves they must be sound fertile have
good dispositions and have good milking
ability they will contribute to the
Cal's weaning weights through their own
milking
ability the donor cow is superovulated
using synchronized injections of
follicle stimulating
hormone during standing estris she is
artificially inseminated with two straws
of
semen 7 days after estrus the embryos
are
collected embryos are evaluated for
viability acceptable embryos are then
Frozen in liquid nitrogen for storage or
shipping prior to transfer the Frozen
embryos are thawed and rehydrated in a
four-step thawing
process the embryos are then transferred
to the recipient females for gestation
and
parturition Embryo transfer is a very
useful procedure that can increase
performance and profitability of
superior cattle Progressive cattle
breeders should consider this procedure
an option when designing New Management
practices to increase the performance of
their herds
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