Cara Menggunakan Spektrofotometer

Summary

This video demonstrates how to use a UV-Vis spectrophotometer for an Android-1 Oroville module experiment. The process includes inserting a sample into a cuvette, ensuring no fingerprints are left on the transparent part, and calibrating the spectrophotometer by measuring a blank. The tutorial walks through measuring absorbance at various wavelengths (645, 552, and 663 nm) for different samples, with clear instructions on adjusting settings, cleaning, and handling the equipment. The goal is to measure the absorbance and transmission of light through the sample, ensuring accurate readings for the experiment.

Takeaways

• 😀 The script demonstrates how to use a UV-Vis spectrophotometer for the Android-1 Oroville module.
• 😀 The spectrophotometer consists of an internal compartment where samples are placed, with different slots for different sample positions.
• 😀 The cuvette, which holds the sample, has two parts: one is clear and the other is frosted. The clear part should never be touched by hand.
• 😀 When filling the cuvette, you do not need to fill it completely, just up to the marked line.
• 😀 Fingerprints should be avoided on the clear part of the cuvette, and any smudges should be wiped off with a tissue.
• 😀 The first sample used is spinach juice, with the same process of handling and inserting into the spectrophotometer.
• 😀 After inserting the sample into the spectrophotometer, make sure the clear part of the cuvette faces the light path.
• 😀 Calibration (blanking) is done to ensure the absorbance is zero before testing samples.
• 😀 After calibration, the absorbance of different wavelengths (645 nm, 552 nm, 663 nm) is measured for each sample.
• 😀 The process involves changing the wavelength settings for each test and reading the corresponding absorbance values for each sample.

Q & A

What type of spectrophotometer is being used in the demonstration?

The demonstration uses a UV-Vis spectrophotometer.

What is the purpose of the cuvette in the spectrophotometer?

The cuvette is a container used to hold the sample for measurement in the spectrophotometer.

What is the correct way to handle the cuvette during the experiment?

The cuvette should be handled by the opaque side to avoid fingerprints, as the clear side must remain clean to ensure accurate readings.

How much liquid should be filled in the cuvette?

The cuvette should be filled up to the marked limit, not completely full.

Why is it important to avoid touching the clear side of the cuvette?

Touching the clear side of the cuvette can leave fingerprints, which may interfere with the light passing through and affect the measurements.

What should be done to clean the cuvette before use?

The cuvette should be cleaned using a wet tissue to remove any fingerprints or contamination.

What is the purpose of the blank sample in the spectrophotometer?

The blank sample is used to calibrate the spectrophotometer, ensuring that the absorbance reading starts at zero before measuring the actual sample.

How do you set the spectrophotometer to zero absorbance?

To set the spectrophotometer to zero absorbance, you need to insert the blank cuvette and calibrate the instrument.

What are the different wavelength values used in the experiment?

The wavelengths used in the experiment are 645 nm, 552 nm, and 663 nm.

How many samples are tested in this procedure?

Two samples are tested in the procedure.