PRAKTIKUM ANALISIS SENYAWA KIMIA (UJI ANTOSIANIN)

Summary

This video script presents an experiment conducted by Sandy from ITB, focused on testing anthocyanins in food materials. The experiment aims to observe how anthocyanin content in various foods like dragon fruit, purple yam, grape skins, cucumber, and tomatoes reacts when treated with NaOH solution. The process involves preparing the materials, creating a NaOH solution, applying it to the food samples, and observing color changes, which indicate the presence of anthocyanins. Notably, the color changes to green or clear, confirming the presence of these compounds in certain fruits and vegetables.

Takeaways

• 😀 Sandy introduces themselves and provides their student ID and university (ITB).
• 😀 The purpose of the experiment is to test the anthocyanin content in food and observe the changes that occur.
• 😀 The tools used in the experiment include a basin, test tubes, motor, pipette, and chopsticks.
• 😀 The materials used in the experiment are dragon fruit, purple yam, grape skins, cucumber, tomato, lye, and distilled water.
• 😀 The first step of the procedure is to prepare the necessary tools and materials for the experiment.
• 😀 The second step involves preparing a 2M NaOH solution by dissolving lye in 500g of water and stirring with chopsticks.
• 😀 The third step is to grind the materials to be tested and place them in separate containers.
• 😀 The fourth step involves adding NaOH solution to the prepared materials using a pipette and observing any changes.
• 😀 After adding NaOH, the fifth step is to observe the color changes in the materials, which indicate the presence of anthocyanins.
• 😀 The materials containing anthocyanins (such as dragon fruit, purple yam, and grape skins) turn green or colorless after treatment with NaOH.

Q & A

What was the main goal of the experiment discussed in the script?

The main goal of the experiment was to test the presence of anthocyanins in food materials and to observe the changes that occur in substances containing anthocyanins.

What were the materials used in the experiment?

The materials used included dragon fruit, purple yam, grape skins, cucumber, tomato, caustic soda (NaOH), and distilled water (aquades).

Which tools were mentioned in the experiment?

The tools mentioned were a basin, test tubes, a mortar, a dropper pipette, and chopsticks.

What safety precaution was emphasized during the experiment?

The script emphasized the importance of not using hands when stirring the caustic soda solution because it could be dangerous if the soda comes into contact with skin.

How was the NaOH solution prepared in the experiment?

The NaOH solution was prepared by dissolving caustic soda in 500 grams of water, creating a 2M solution, which was then stirred using chopsticks.

What was the next step after preparing the NaOH solution?

The next step was to crush the materials (such as fruits and vegetables) and place them into separate containers for testing.

What happened when NaOH was added to the different substances?

When NaOH was added to the substances, changes in color were observed, particularly in materials containing anthocyanins, which turned green and then clear.

What was the significance of observing color changes in the substances?

The color changes, particularly turning green and clear, indicated the presence of anthocyanins in the materials, such as dragon fruit, purple yam, and grape skins.

How were the substances prepared for testing before adding NaOH?

The substances were first crushed and placed into separate containers, after which NaOH was added drop by drop to each substance.

Why was it important to observe the changes in the materials after NaOH was added?

Observing the changes allowed the experimenters to determine how substances containing anthocyanins reacted to the NaOH solution, providing insight into the chemical properties of anthocyanins.