PEMBUATAN LARUTAN STOK F MEDIA MS // KULTUR JARINGAN

Summary

In this video, Roni Kartiman and Mbak Putri demonstrate the process of preparing a special solution for plant tissue culture. The solution, labeled 'F', involves the careful preparation of two chemicals, Na2EDTA and FeSO4, which must be dissolved separately to avoid clumping. The process requires precise measurements, hot water, and magnetic stirring to achieve proper dissolution. Once the solutions are combined, they are stored in a light-protective bottle wrapped in aluminum foil. The video offers a step-by-step guide to creating a crucial solution for plant tissue culture research.

Takeaways

• 😀 The video begins with a greeting and introduction to the topic, which is the preparation of solution F for plant tissue culture in a laboratory.
• 😀 Solution F contains two primary components: Na2EDTA (3.73 grams) and FeSO4 (2.78 grams), which need to be dissolved separately before being combined.
• 😀 Both components must be dissolved with the help of warm water or a magnetic stirrer with a heating function.
• 😀 It is crucial that each component is dissolved in 400 ml of water before combining, to prevent clumping, which could occur if mixed directly.
• 😀 Na2EDTA and FeSO4 should be dissolved in separate containers with warm water, ensuring no direct contact between the two until both are fully dissolved.
• 😀 After dissolving, the solutions are combined to form a 1-liter solution of Solution F.
• 😀 The video emphasizes that the solution must be kept in containers that are covered with aluminum foil to protect it from light exposure, which can affect the solution.
• 😀 The temperature of the water used for dissolving the chemicals should be around 120°C, and the process should be done carefully to avoid any oversaturation.
• 😀 Once combined, the solution is stirred to ensure it is homogeneous, ensuring uniformity for use in plant tissue culture.
• 😀 The video ends with a brief mention of the storage and usage of the solution, with the reminder that the solution should be used in a dark environment and that 10 ml of the solution is typically used per liter of culture medium.

Q & A

What is the main focus of the video script?

The main focus of the video is to demonstrate the proper preparation of 'Larutan F', a solution used in plant tissue culture, specifically showing how to dissolve Na2EDTA and FeSO4 without causing clumping.

Why should Na2EDTA and FeSO4 be dissolved separately?

Na2EDTA and FeSO4 should be dissolved separately because if they are combined directly, the solution will clump, leading to an unsuccessful preparation.

What are the required amounts for Na2EDTA and FeSO4?

The required amount for Na2EDTA is 3.73 grams, and for FeSO4, it is 2.78 grams.

How should the Na2EDTA and FeSO4 be dissolved?

Both Na2EDTA and FeSO4 should be dissolved separately in hot water or using a magnetic stirrer with a heating function to aid in the dissolution process.

What is the total volume of the final solution after combining both dissolved substances?

The final volume of the solution should be 1 liter after both dissolved substances are combined and adjusted to the correct volume.

Why is it important to maintain the solution at a specific temperature during preparation?

Maintaining the solution at a specific temperature, around 120°C, helps dissolve the substances effectively without clumping, ensuring a smooth and homogeneous solution.

What should be done to the solution after it has been prepared and before storing it?

After preparation, the solution should be stored in a bottle wrapped in aluminum foil to prevent exposure to light, as the solution is sensitive to light.

What is the purpose of the magnetic stirrer in the preparation process?

The magnetic stirrer is used to ensure even mixing and to help dissolve the substances thoroughly by providing continuous agitation during the heating process.

What is the function of the aluminum foil wrapping on the bottle?

The aluminum foil wrapping prevents the solution from being exposed to light, which could affect its stability or effectiveness.

How much of the solution should be used when preparing media for tissue culture?

When preparing media for tissue culture, 10 milliliters of the 'Larutan F' solution should be used per liter of media.