Modul 7 : Disolusi || Praktikum Farmasi Fisik || Farmasi ITERA

Summary

The video script details a scientific procedure involving the preparation and testing of salicylic acid solutions. It outlines steps such as measuring and dissolving salicylic acid, adjusting temperatures and speeds in specific equipment, and conducting regular sample measurements. Absorbance values of the samples are measured using a spectrophotometer, with frequent rinsing and careful attention to the setup of the cuvette. The process includes creating a series of solutions with varying concentrations and testing them under controlled conditions. The detailed procedure ensures accurate results for the analysis of salicylic acid in the solution.

Takeaways

• 😀 Salicylic acid is weighed and prepared for testing in a solution tester.
• 😀 Distilled water is added to the chambers, and the solution tester is powered on for adjustments.
• 😀 The temperature is set to 30°C, and the speed is adjusted to 50 rpm in the solution tester.
• 😀 Salicylic acid (2 grams) is added to each chamber at specific intervals (1st, 5th, and 10th minute).
• 😀 Every 5 and 10 minutes, 10 ml of water is taken from each chamber and replaced with 10 ml of distilled water.
• 😀 0.1 grams of salicylic acid is measured and dissolved in 100 ml of aquades in a volumetric flask.
• 😀 From the main salicylic acid solution, serial dilutions are made to create solutions with different concentrations (80-100, 120-140, and 160 cm).
• 😀 A cuvette is cleaned three times using distilled water before being filled with distilled water as a blank sample.
• 😀 The blank cuvette is placed in the spectrophotometer to set the baseline for absorbance measurements.
• 😀 After calibrating with the blank, the cuvette containing the sample is placed in the spectrophotometer to measure its absorbance.
• 😀 Absorbance measurements are repeated multiple times, and each sample is cleaned three times with distilled water between measurements.

Q & A

What is the first step in the procedure mentioned in the script?

The first step involves preparing a solution by weighing 2 grams of salicylic acid.

What is the purpose of the solution Playstore mentioned in the script?

The solution Playstore is used to set up and operate the device that conducts the experiment, ensuring the chambers are properly prepared for the test.

What is the role of the distillation water in the process?

Distilled water is used to replace samples taken from each chamber during the experiment to maintain consistent conditions.

How is the solution tester activated in the procedure?

The solution tester is activated by pressing the on-off button located at the back of the device.

What specific temperature and speed settings are used during the experiment?

The temperature is set to 30°C, and the speed is set to 50 rpm.

When should the 2 grams of salicylic acid be added to the chambers?

The 2 grams of salicylic acid should be added to each chamber once the temperature reaches 30°C.

What is the purpose of measuring the absorbance of the sample?

The absorbance is measured to analyze the concentration of the solution, indicating the presence and amount of the substance in the sample.

How are the standard solutions of acetylsalicylic acid prepared in the script?

The standard solutions of acetylsalicylic acid are prepared by creating serial dilutions with concentrations of 80-100, 120-140, and 160 cm.

What steps are involved in preparing the cuvette for the spectrophotometer?

The cuvette is first washed three times with distilled water, then filled with distilled water as a blank. The sample solution is added to the cuvette for measurement.

Why is it important to repeat the absorbance measurement multiple times?

Repeating the absorbance measurement helps ensure accuracy and consistency in the results by minimizing experimental errors.