Benzidine test || Blood in urine || Hematuria || #Biochemistry

Summary

This video demonstrates an incident test for blood presence in urine using hydrogen peroxide solution. The process involves adding a small urine sample to the solution, and if blood is present, the color of the solution changes to green. The test relies on the interaction between hemoglobin in blood and hydrogen peroxide. The video also touches on clinical applications, including the identification of urinary tract issues such as kidney stones, infections, and conditions like sickle cell anemia. Viewers are encouraged to like, share, and subscribe for more informative content on health-related topics.

Takeaways

• 😀 Urine samples are used to test for the presence of blood in the body.
• 😀 Hydrogen peroxide (H2O2) solution is added to the urine sample to detect blood.
• 😀 If blood is present, the urine sample will turn a greenish color due to a chemical reaction.
• 😀 The test works by hydrogen peroxide reacting with hemoglobin in blood, creating a color change.
• 😀 The presence of blood in urine (hematuria) can indicate several medical conditions.
• 😀 Common conditions associated with blood in urine include urinary tract infections (UTIs) and kidney stones.
• 😀 Other potential causes of blood in urine include bladder stones and inherited conditions like sickle cell anemia.
• 😀 Chronic kidney diseases and certain medications can also lead to blood in urine.
• 😀 This test provides a quick and simple method for detecting blood in the urine sample.
• 😀 It is important to seek medical advice if the test indicates blood in urine for further diagnosis and treatment.
• 😀 The video encourages viewers to like, share, and subscribe for more informative health-related content.

Q & A

What is the purpose of the incident test mentioned in the video?

The incident test mentioned in the video is designed to detect blood in urine, helping identify potential underlying health issues.

What is the required sample for this test?

The test requires a urine sample, though blood can also be used. The urine sample should be 30 mL, and the blood test can be considered if needed.

What is the role of hydrogen peroxide (H2O2) in this test?

Hydrogen peroxide (H2O2) is used as a solution that reacts with hemoglobin present in the blood, helping to detect its presence in the urine sample.

What is the expected result if blood is present in the urine sample?

If blood is present in the urine, the solution will turn a green color due to the chemical reaction between hydrogen peroxide and hemoglobin.

What clinical conditions can be associated with the presence of blood in urine?

The presence of blood in urine can be linked to conditions such as urinary tract infections, kidney stones, bladder stones, and prostate issues, among others.

What is the principle behind the test for detecting blood in urine?

The test relies on the reaction between hydrogen peroxide and hemoglobin, which breaks down the peroxide into water and oxygen, resulting in a color change that signals the presence of blood.

How does the test help in identifying urinary tract infections (UTIs)?

The test can help identify UTIs by detecting blood in the urine, which is a common symptom of these infections.

What role does hemoglobin play in this test?

Hemoglobin, a protein found in red blood cells, reacts with hydrogen peroxide during the test. The presence of hemoglobin indicates blood in the urine, which may point to various medical conditions.

How should the test be conducted according to the video?

To conduct the test, place 5 mL of urine in a test tube, add two drops of the hydrogen peroxide solution, and observe the color change. If blood is present, the solution will turn green.

What is the significance of the green color in the test result?

The green color indicates that blood is present in the urine sample, signaling a potential underlying health issue that may require further investigation.