Video Kultur Primer Sel Fibroblas- Program Studi Magister Ilmu Biomedik FK-KMK UGM

Summary

In this practical tutorial, the process of fibroblast cell culture is demonstrated, focusing on isolating cells from rat dorsal tissue. The cells are placed in a controlled environment to grow and studied for their cellular and biochemical properties. The video emphasizes the importance of regularly monitoring the culture for contamination, changing the culture medium, and observing cell growth over time. This practice helps understand cell behavior, making it crucial for research on tissue repair and cellular responses in a lab setting.

Takeaways

• 😀 Cell culture involves extracting cells from plant or animal tissues and placing them in a controlled environment for growth.
• 😀 The session focuses on primary cell culture, particularly fibroblast cells extracted from rat back tissue.
• 😀 The main objective is to observe the cellular and biochemical characteristics of the cultured cells over time.
• 😀 Regular observation of the cells is required to monitor their growth and health during the culture process.
• 😀 The culture medium must be changed periodically to ensure healthy cell growth and to prevent contamination.
• 😀 Contamination in cell culture should be closely monitored to ensure the integrity of the experiment.
• 😀 The video highlights the importance of a conducive environment for cell growth and the accurate monitoring of the culture.
• 😀 By observing fibroblast cells, we can gain insights into cell physiology and biochemistry.
• 😀 The process involves separating the cells from the tissue and placing them in a suitable environment for culturing.
• 😀 The goal is to grow and study cells in an artificial environment, gaining valuable information on their behavior and characteristics.

Q & A

What is the purpose of the cell culture practical described in the video?

The purpose of the cell culture practical is to observe the growth and behavior of fibroblast cells taken from rat tissue, and to study their physiological and biochemical characteristics in a controlled environment.

What type of cells are being cultured in the video?

The cells being cultured are fibroblast cells, which are taken from the back tissue of rats.

What is the goal of separating cells from their tissue?

The goal of separating the cells from their tissue is to grow them in a controlled environment, allowing for detailed study of their cellular and biochemical properties.

How does the video suggest maintaining the cultured cells?

The video suggests regularly changing the medium and monitoring the culture for any signs of contamination to ensure the cells grow properly.

What should be observed during the cell culture process?

During the cell culture process, observations should focus on the cells' physiological behavior, the potential for contamination, and their overall growth and health over time.

Why is it important to monitor the cell culture periodically?

It is important to monitor the cell culture periodically to ensure the cells are growing well, detect any contamination early, and replace the medium when necessary.

What happens if contamination occurs in the cell culture?

If contamination occurs, it could interfere with the growth and health of the cells, potentially leading to inaccurate observations and unreliable results.

What does the video indicate is required to ensure proper growth of the cultured cells?

The video indicates that the cells require a conducive environment, proper medium replacement, and periodic observation to ensure their growth and proper development.

What is the significance of using fibroblast cells in this practical?

Fibroblast cells are used because they are commonly studied for their role in tissue repair and growth, making them valuable for understanding cellular physiology and biochemistry in lab settings.

What is the next step after observing the cultured cells over time?

After observing the cultured cells over time, the next step involves analyzing their physiological and biochemical properties to better understand their behavior and response to the culture conditions.