Metode ekstraksi dan isolasi piperin

Summary

The video outlines a practical session on isolating piperine from black pepper (Piper nigrum), emphasizing the extraction process using sokletasi with ethanol. Students learn to extract piperine, which exhibits pharmacological benefits, and to purify it into yellow crystals. The procedure includes washing the crystals and confirming their identity through thin-layer chromatography (TLC) using Dragendorff's reagent. The session effectively demonstrates both the methodology and the significance of piperine, engaging students in practical lab techniques and the understanding of its chemical properties.

Takeaways

• 😀 The practical session focuses on the isolation of piperine from black pepper (Piper nigrum).
• 🌱 Piperine is the main compound in black pepper and has significant pharmacological benefits.
• 🔬 The extraction method used is Soxhlet extraction, which is efficient for isolating compounds.
• 💧 Ethanol 96% is utilized as a solvent during the extraction process.
• ⏳ The extraction typically lasts between 1-2 hours, involving multiple cycles for optimal yield.
• ⚗️ After extraction, the black pepper extract is concentrated using a rotary evaporator.
• 🧪 The addition of 10% KOH ethanolic solution helps to purify the piperine extract.
• ❄️ The isolated piperine crystals are washed with cold ethanol to remove impurities.
• 📊 Identification of piperine is performed using thin-layer chromatography (TLC) with a silica gel plate.
• 🔍 The Dragendorff reagent is used for visualizing piperine on the TLC plate to confirm its presence.

Q & A

What is the main objective of the practical session described in the transcript?

The main objective is to understand the principles of isolation and to isolate piperine from black pepper (Piper nigrum).

What is piperine, and why is it significant?

Piperine is the primary active compound found in black pepper, known for its pharmacological benefits and importance as a spice.

What method is used for the extraction of piperine in this practical?

The method used for extraction is Soxhlet extraction, which is noted for its efficiency.

How much black pepper powder is used in the Soxhlet extraction process?

10 grams of black pepper powder is used in the Soxhlet extraction process.

What solvent is employed in the Soxhlet extraction of piperine?

96% ethanol is used as the solvent in the Soxhlet extraction of piperine.

How long is the Soxhlet extraction typically performed?

The Soxhlet extraction is typically performed for 1-2 hours with about 6-15 cycles of circulation.

What happens to the extract after the Soxhlet extraction is completed?

After extraction, the liquid extract is evaporated using a rotary evaporator until about 5 mL of concentrated extract remains.

What is the purpose of adding ethanolic KOH to the extract?

Ethanolic KOH is added to facilitate the reaction and help separate the piperine from the residue.

How is the purity of the isolated piperine confirmed?

The purity of the isolated piperine is confirmed using thin-layer chromatography (TLC) and the Dragendorff reagent.

What is the significance of washing the piperine crystals with cold ethanol?

Washing the piperine crystals with cold ethanol helps to remove impurities and achieve a cleaner product.