Aktivitas 4. Transport Membran-Filtrasi

Summary

The video script discusses a lab activity focused on studying the process of filtration through a cell membrane. It explains the steps, including preparing solutions with solutes like sodium chloride, urea, glucose, and carbon, and using membranes with different pore sizes to observe filtration rates. The activity measures how solutes filter under various pressures and times, with detailed instructions on analyzing membranes for residual solutes. The script ends with quiz questions related to the filtration process, asking about factors that influence filtration and physiological changes in the body.

Takeaways

• 🧪 The activity focuses on studying the filtration process using a cell membrane.
• 📏 Equipment includes a membrane, solutes, and two beakers.
• 🔬 The experiment uses sodium chloride, urea, glucose, and charcoal powder at a concentration of 5 mg/ml.
• ⚙️ The filtration process is conducted with 50 mm Hg pressure over 60 minutes.
• 📝 After filtration, the membrane is analyzed to detect the presence of solutes.
• ❓ The first question asks why no solutes are found in the filtrate, with multiple choice options based on filtration pressure, solute size, concentration, and energy use.
• 🔍 A prediction question asks about the effect of increasing membrane pore size on filtration rate.
• 🔧 Different membrane sizes (20 MWCO, 50 MWCO, 200 MWCO) are tested during the experiment.
• ⏳ Increasing the pressure to 100 mm Hg leads to faster filtration, and another question predicts the impact on filtrate concentration.
• 💡 The final question involves identifying the physiological response (e.g., heart rate, body temperature, or blood pressure) to filtration within the body.

Q & A

What equipment is used in the filtration process described in the script?

The equipment includes solutes (sodium chloride, urea, glucose, and charcoal), a membrane with a size of 20 MWCO, two beakers, and a filtration system that applies pressure.

What is the role of the membrane in the filtration process?

The membrane acts as a filter between two beakers, allowing smaller molecules to pass through while trapping larger solutes based on their size.

What is the concentration of solutes used in the experiment?

The concentration of each solute (sodium chloride, urea, glucose, and charcoal) is 5 mg/mL.

What pressure is applied during the filtration process?

The pressure applied during the filtration process is 50 mm Hg.

How long does the filtration process take?

The filtration process takes 60 minutes in the initial experiment.

What happens to the membrane after the filtration process is complete?

After filtration, the membrane is analyzed for the presence of solutes, and the data is recorded.

Why might solutes not pass through the membrane during filtration?

Solutes may not pass through the membrane if their size is too large, if the filtration pressure is too low, if the solute concentration is too low, or if there is insufficient energy for filtration.

What effect does increasing the pore size of the membrane have on the filtration rate?

Increasing the pore size of the membrane generally increases the filtration rate, allowing more solutes to pass through more quickly.

What happens when the filtration pressure is increased?

Increasing the filtration pressure can increase the filtration rate and potentially increase the concentration of solutes in the filtrate.

What physiological response was mentioned in the script's final question regarding driving forces in the body?

The final question refers to physiological responses such as increased heart rate, body temperature, faster breathing, or increased blood pressure as potential driving forces.