Common Lab Techniques Video

Matthew Campbell
15 Oct 201314:49

Summary

TLDRThis chemistry lab tutorial introduces essential laboratory techniques for an introductory chemistry course. It covers the proper use of an electronic balance for precise mass measurement, the importance of using weighing boats or paper, and the correct operation of a Bunsen burner for safety and temperature control. The video also demonstrates how to measure liquid volumes with a graduated cylinder, take accurate temperature readings with a thermometer, and perform filtration to separate mixtures. Each technique is explained with a focus on safety and accuracy, ensuring students are well-prepared for lab work.

Takeaways

  • πŸ”¬ Chemistry is the study of matter and its changes, and laboratory techniques are crucial for understanding these processes.
  • πŸ“ Proper use of an electronic balance is essential, with sensitivity to one-hundredth of a gram and a maximum capacity of 400 grams.
  • 🚫 Avoid placing substances directly on the balance pan to prevent contamination and ensure accurate measurements.
  • βš–οΈ Always zero the balance before use and use a weighing boat or paper to measure the mass of substances.
  • 🌑️ Measuring specific volumes of liquids accurately requires the use of graduated cylinders, which are more precise than beakers or flasks.
  • πŸ’§ When measuring liquids, ensure the meniscus is at eye level for accurate readings and select the appropriate cylinder size for the volume needed.
  • 🌑️ Accurate temperature measurements involve submerging the thermometer fully without touching the container walls and estimating to the nearest tenth of a degree.
  • πŸ§ͺ Filtration is a technique used to separate mixtures, requiring a funnel, filter paper, and a beaker, with careful pouring to avoid spillage.
  • πŸ”₯ Safety is paramount when using a Bunsen burner, including removing combustibles, securing loose clothing, and using safety goggles and an apron.
  • πŸ”₯ Correctly lighting and adjusting a Bunsen burner involves opening the gas valve, making a spark, and adjusting the combustion column for a blue flame.

Q & A

  • What is the primary focus of chemistry as a science?

    -Chemistry is the science of the study of matter and the changes that matter undergoes.

  • Why are electronic balances sensitive instruments in a chemistry lab?

    -Electronic balances are sensitive because they are designed to measure mass to the nearest one-hundredth of a gram and have a capacity of only 400 grams.

  • What is the proper way to turn on an electronic balance?

    -You can turn on an electronic balance by holding the on button for several seconds or by pressing the on zero button quickly to rezero or tare it.

  • Why should you not place substances directly on the metal pan of a balance?

    -To prevent the substance from coming in contact with the pan, you should use a plastic weighing boat or dish or a piece of weighing paper.

  • How can you ensure the balance is correctly zero before measuring the mass of an object?

    -Before placing an object on the pan, ensure the balance is correctly zero by either turning it on or pressing the zero button to tare it.

  • What is the significance of the negative mass displayed by the balance when the weighing dish is removed?

    -A negative mass indicates that the balance is subtracting the weight of the plastic weighing dish, which means it has been successfully zeroed.

  • Why should you not touch chemical substances directly with your fingers in a lab?

    -Direct contact with chemical substances should be avoided to prevent contamination and ensure safety; use tools like a spoon or other apparatus designed for dispensing chemicals.

  • How can you accurately measure a specific volume of a liquid in a chemistry lab?

    -Use a graduated cylinder, ensuring it is the right size for the volume you need to measure, and check the meniscus at eye level for precise readings.

  • What is the purpose of swirling a mixture of water, salt, and sand in a beaker?

    -Swirling helps the salt dissolve and allows the sand to settle to the bottom of the beaker.

  • How should you handle a thermometer for accurate temperature readings in a chemistry lab?

    -Submerge the thermometer as far as possible into the material without touching the container walls, and use a stand with a clamp for longer measurements.

  • What is the importance of filtration in a chemistry lab and how is it set up?

    -Filtration is used to separate mixtures, such as transferring sand into filter paper while allowing salt water to flow through. It involves setting up a funnel with filter paper, supported by a triangle and a stand.

  • What safety precautions should be taken when lighting a Bunsen burner in a chemistry lab?

    -Ensure the area is clear of combustible materials, wear appropriate safety gear, and adjust the gas valve and combustion column correctly before lighting.

Outlines

00:00

πŸ”¬ Introduction to Chemistry Lab Techniques

This paragraph introduces the viewer to common laboratory techniques used in an introductory chemistry course. It emphasizes the importance of understanding chemistry as the study of matter and its changes. The script discusses the correct use of an electronic balance for measuring the mass of substances with precision up to one-hundredth of a gram. The balance's sensitivity and cost are highlighted, cautioning against misuse. Instructions are provided on how to turn on the balance, zero it, and use it with a plastic weighing boat or paper to prevent direct contact with the pan. The process of measuring the mass of substances, subtracting the weight of the container, and adding chemicals using appropriate tools without direct contact are explained. The paragraph concludes with a practical exercise to measure specific masses of NaCl and sand, emphasizing cleanliness and preparation for the next user.

05:00

πŸ“ Measuring Volumes and Temperatures in the Lab

The second paragraph focuses on measuring specific volumes of liquids using graduated cylinders, emphasizing the importance of selecting the right tool for the job. It provides a step-by-step guide on how to accurately measure 95.0 milliliters of water, including tips on using a squirt bottle and reading the meniscus correctly. The paragraph also covers the technique of mixing substances, such as salt and sand with water, to create a beach-like mixture. Additionally, it discusses the use of thermometers for measuring the temperature of substances, detailing how to obtain accurate readings and safety considerations, such as not leaving thermometers unsupported. The importance of estimating to the nearest tenth of a degree is mentioned, with an example of a 20.0 degrees Celsius reading. The viewer is instructed to measure the temperature of their mixture and record it before proceeding.

10:02

πŸ§ͺ Filtration and Bunsen Burner Safety

The final paragraph covers the process of filtration to separate mixtures, such as sand from saltwater, using a funnel, filter paper, and a glass rod. It describes how to set up the filtration equipment, including the use of a triangle and a ring stand, and the importance of folding the filter paper correctly to create a cone shape that fits into the funnel. The technique of decanting is introduced, where the glass rod is used to direct the flow of liquid into the funnel. The paragraph concludes with a discussion on safely lighting and adjusting a Bunsen burner, including safety precautions, such as removing combustible materials and securing loose clothing. It provides a detailed guide on connecting the burner to the gas jet, lighting it with a striker, and adjusting the combustion column to control the flame's temperature and color. The viewer is encouraged to demonstrate their proficiency with the Bunsen burner and, if time permits,蒸发 the water from a previously filtered saltwater mixture to separate the salt.

Mindmap

Keywords

πŸ’‘Chemistry

Chemistry is the scientific study of the composition, structure, properties, and reactions of matter. In the context of the video, chemistry is the overarching theme as it discusses laboratory techniques used in an introductory chemistry course. The script mentions that chemistry is the science of studying matter and its changes, which is central to the experiments and techniques being taught.

πŸ’‘Laboratory Techniques

Laboratory techniques refer to the methods and procedures used in a laboratory setting to conduct scientific experiments and analyses. The video script introduces various common techniques such as using an electronic balance, graduated cylinders, thermometers, and Bunsen burners, which are essential for performing experiments in chemistry.

πŸ’‘Electronic Balance

An electronic balance is a sensitive instrument used to measure the mass of substances with precision. The script emphasizes the importance of using an electronic balance correctly, such as not overloading it or placing substances directly on its pan, to ensure accurate measurements. It is a critical tool for weighing chemicals in the lab.

πŸ’‘Graduated Cylinder

A graduated cylinder is a piece of laboratory glassware used for measuring the volume of liquids. The script explains the correct use of a graduated cylinder to measure a specific volume of liquid, such as water, with precision. It is highlighted as a better tool for volume measurement compared to beakers or flasks.

πŸ’‘Thermometer

A thermometer is an instrument used to measure temperature. In the video, the proper use of a thermometer to obtain accurate temperature readings of substances is discussed. It is crucial for experiments where temperature control or measurement is a factor.

πŸ’‘Filtration

Filtration is a laboratory technique used to separate solids from liquids or to separate particles based on size. The script describes the process of filtration, including the setup of equipment like a funnel, filter paper, and beaker, to separate sand from a saltwater solution.

πŸ’‘Bunsen Burner

A Bunsen burner is a common piece of laboratory equipment that provides a flame for heating substances. The script covers the safety considerations and the correct procedure for lighting and adjusting a Bunsen burner, which is essential for experiments involving heat.

πŸ’‘Weighing Boat

A weighing boat, or dish, is used to hold substances during weighing on an electronic balance. The script mentions the use of a plastic weighing boat to prevent direct contact between the substance and the balance pan, ensuring cleanliness and accuracy in measurements.

πŸ’‘Meniscus

The meniscus is the curved surface of a liquid in response to its container or to gravity. When measuring liquid volume with a graduated cylinder, the script instructs to read the volume at the bottom of the meniscus to ensure an accurate measurement.

πŸ’‘Decanting

Decanting is the process of transferring a liquid from one container to another, often to separate it from a solid. The script describes using a glass rod to direct the flow of saltwater into a funnel during the filtration process, which is a form of decanting.

πŸ’‘Safety Precautions

Safety precautions are essential measures taken to prevent accidents in a laboratory setting. The script highlights several safety considerations, such as removing combustible materials and wearing safety goggles when using a Bunsen burner, to ensure a safe laboratory environment.

Highlights

Introduction to common laboratory techniques in chemistry.

Importance of proper use and care of an electronic balance for accurate mass measurements.

Avoiding damage to sensitive electronic balances by not applying excessive force.

Instructions on turning on and zeroing an electronic balance.

Using plastic weighing boats or paper to prevent direct contact with the balance pan.

Technique of taring the balance to subtract the weight of the container.

Proper handling of chemical substances to avoid direct contact with hands.

Measuring specific volumes of liquids using graduated cylinders.

Selecting the appropriate size of graduated cylinder for accurate volume measurements.

Correct technique for reading volumes at eye level and from the bottom of the meniscus.

Combining measured substances to create a mixture and observing the dissolution and settling process.

Measuring the temperature of substances using a thermometer and ensuring accurate readings.

Safety precautions when handling thermometers to prevent accidents in the lab.

Estimating readings to the nearest decimal place smaller than the scale's increment.

Filtration technique for separating mixtures using a funnel, filter paper, and support stand.

Proper folding and placement of filter paper in the funnel for effective filtration.

Decanting technique to transfer liquid through the filter paper without spilling.

Completing the filtration process and separating sand from salt water.

Safety measures and proper lighting of a Bunsen burner in the chemistry lab.

Adjusting the Bunsen burner for optimal flame temperature and color.

Conclusion of the laboratory techniques practice with a focus on safety and accuracy.

Transcripts

play00:08

during your study of chemistry you will

play00:10

be working in a laboratory setting to

play00:12

perform various experiments the purpose

play00:15

of this information is to introduce some

play00:17

of the most common techniques that will

play00:18

be used throughout the year and give you

play00:21

a chance to practice them chances are

play00:24

that your teacher has already discussed

play00:26

with you that chemistry is the science

play00:28

of the study of matter and the changes

play00:30

that matter undergoes during this lab we

play00:33

will look at various techniques that

play00:36

will be used throughout the year and we

play00:38

will examine two questions specifically

play00:40

first what are the most common

play00:42

laboratory tools and techniques used in

play00:45

an introductory chemistry course and

play00:46

second how are these tools and

play00:49

techniques used and performed

play00:50

effectively one of the most common

play00:55

techniques in any laboratory is the

play00:57

ability to measure the mass of a

play00:59

substance using an electronic balance in

play01:02

this section we will look at the proper

play01:04

use and operation of an electronic

play01:07

balance before we talk about how to use

play01:10

the balance let's first talk about what

play01:12

not to do with the balance the balances

play01:15

that you will be using in chemistry lab

play01:17

are sensitive instruments most of them

play01:20

are designed to measure mass to the

play01:21

nearest one one hundredth of a gram and

play01:24

most of them have a capacity of only 400

play01:27

grams which is less than a pound so

play01:30

please resist the urge to test your

play01:32

superhuman strength by pushing down on

play01:34

the pan of the balance to see how high

play01:37

the numbers will go they won't go very

play01:39

high and you'll break the balance these

play01:41

balances cost about three to four

play01:43

hundred dollars a piece so they're

play01:45

fairly expensive to replace step one in

play01:50

using the electronic balance involves

play01:51

turning the balance on or tearing or re0

play01:54

in the balance both of these processes

play01:57

can be done by either holding the on

play02:00

button for several seconds if you want

play02:02

to turn the balance on or off or simply

play02:05

pressing quickly the on zero button if

play02:08

you want the balance to rezero or tear

play02:11

it

play02:11

before placing anything on the pan of

play02:14

the balance to determine its mass you

play02:17

should make sure that the balance is

play02:18

correctly zero one rule when using any

play02:24

balance is to never place a substance

play02:26

directly on the metal pan of the balance

play02:28

instead we should use a plastic weighing

play02:31

boat or dish or a folded piece of

play02:34

weighing paper to prevent the substance

play02:36

from coming in contact with the pan when

play02:39

that weighing boat or paper is placed on

play02:42

the balance the balance does what it's

play02:44

designed to do it determines the mass of

play02:46

any object placed on the pan so notice

play02:49

now the balance is telling us the mass

play02:52

of that plastic weigh boat

play02:55

at this point if we simply press the

play02:59

zero button the balance will

play03:01

automatically Reiser o itself and

play03:03

subtract the weight of the plastic

play03:06

weighing dish most people find this to

play03:09

be easier than recording the mass of the

play03:11

dish and then subtracting it from the

play03:14

mass of the dish and the compound notice

play03:18

if we remove the dish the balance shows

play03:20

a negative mass which is telling us that

play03:23

the balance is subtracting the weight of

play03:25

that plastic weighing dish which is two

play03:28

point zero two grams at this point we

play03:33

can place the plastic weighing dish back

play03:35

on the balance and start adding to it

play03:37

whatever substance we wish to measure

play03:39

the mass of keep in mind that these are

play03:42

chemical substances and we should never

play03:44

touch them directly with our fingers we

play03:46

should always use a spoon or some other

play03:48

apparatus specifically designed for

play03:51

dispensing chemicals to add the

play03:53

substance to the plastic weighing dish

play03:58

once you have obtained the desired mass

play04:00

of a particular substance you're ready

play04:02

to remove the plastic dish from the

play04:04

balance and take it back to your lab

play04:06

table area to carry on the rest of the

play04:10

procedure if you spilled any substance

play04:12

around the balance now would be the time

play04:14

to clean up that substance so that the

play04:16

balance area is ready for the next

play04:19

person who needs to use it at this point

play04:23

you're ready to try part number one of

play04:25

the practicing laboratory techniques

play04:27

procedure that can be found in your lab

play04:29

manual notice the procedure asked you to

play04:32

measure two separate substances with a

play04:35

specific mass 0.5 zero grams of NaCl

play04:38

which is common table salt and point

play04:41

five zero grams of sand use the

play04:43

techniques we just discussed and show

play04:46

your teacher when you have completed

play04:47

this step another common technique used

play04:52

in the chemistry laboratory is being

play04:54

able to measure a specific volume of a

play04:56

liquid using a graduated cylinder an

play05:00

important part of doing any job

play05:02

correctly is selecting the right tool

play05:04

for the job that you want to complete

play05:07

lots of different glassware in the

play05:09

laboratory can be used to measure volume

play05:11

but remember graduated cylinders do that

play05:14

job much better than beakers and flasks

play05:19

since our goal is to measure exactly

play05:21

ninety 5.0 milliliters of water we want

play05:25

to be sure to select the appropriate

play05:26

size graduated cylinder we're choosing

play05:29

to use one that can measure a maximum

play05:32

volume of 100 milliliters you may find

play05:35

it easier to take the cap off the

play05:37

deionized water squirt bottle and pour

play05:40

the volume of water to about ninety

play05:43

milliliters then you can fill up the

play05:47

remaining volume using the fine tip of

play05:50

the squirt bottle to level the volume at

play05:52

95 milliliters remember to carefully

play05:57

breathe the volume on the graduated

play06:00

cylinder at eye level and remember to

play06:02

measure from the bottom of the meniscus

play06:04

formed by the surface of the water not

play06:07

the top

play06:09

at this point you should have measured

play06:11

point five zero grams of salt and point

play06:13

five zero grams of sand and they should

play06:16

be in a 250 milliliter beaker you just

play06:19

finished measuring ninety five point

play06:21

zero milliliters of water and at this

play06:23

point you're ready to combine those

play06:25

substances by combining the water with

play06:29

the salt and sand you've created a

play06:32

mixture swirling will allow the salt to

play06:34

dissolve and the sand will settle to the

play06:37

bottom of the beaker you've just created

play06:40

a day at the beach a third technique

play06:44

that is often employed in a chemistry

play06:46

laboratory is measuring the temperature

play06:48

of a substance or material using a

play06:51

thermometer in order to obtain the most

play06:55

accurate temperature reading possible

play06:57

the thermometer must be submerged as far

play07:00

as possible

play07:01

into the material without touching the

play07:03

walls of the container for this

play07:07

particular experiment and anytime you

play07:09

need to take a quick measurement you can

play07:11

simply hold the thermometer by hand but

play07:14

for longer periods of time you may be

play07:16

better off using a stand with a clamp

play07:19

attached specifically designed to hold a

play07:22

thermometer one lab safety issue to be

play07:26

aware of is never to allow a thermometer

play07:28

to rest in a beaker or other container

play07:31

unsupported if you bump into it it will

play07:33

easily tip over

play07:35

so if using a thermometer and holding it

play07:37

by hand be sure to set it down flat on

play07:40

the countertop when you are finished

play07:44

remember when breeding any instrument

play07:47

with a printed scale we are always

play07:49

allowed to estimate one decimal place

play07:51

smaller than the scale that is printed

play07:53

so in this particular case the scale is

play07:57

in one degree Celsius increments so we

play07:59

can estimate to the nearest tenth of one

play08:02

degree in this particular case our

play08:04

reading would be twenty point zero

play08:06

degrees Celsius so right now you should

play08:09

measure the temperature of your water

play08:11

salt and sand mixture and record this

play08:14

value have your teacher check this value

play08:17

before moving on another common

play08:21

technique to master in

play08:22

the lab is the separation of a mixture

play08:25

using filtration the process of

play08:29

filtration will involve a more

play08:32

complicated equipment setup compared to

play08:34

what we've used up into this point

play08:36

notice in the picture a funnel is being

play08:39

supported by a triangle which in turn is

play08:42

supported by a rink Lant to a stand

play08:45

which rests on the lab table underneath

play08:47

the funnel is a clean empty beaker that

play08:50

we're going to use to catch the salt

play08:52

water that runs through the filter paper

play08:56

by itself the funnel is not a good tool

play08:59

to use to separate sand from salt where

play09:02

in order to do this process more

play09:04

effectively we will need to use a piece

play09:07

of filter paper in order to get the

play09:10

filter paper which is flat to fit into

play09:13

the funnel which is not we have to do a

play09:15

little paper folding first fold the

play09:18

paper in half then fold the paper in

play09:21

half again

play09:25

finally notice if you look at the curved

play09:28

end of your folded filter paper you

play09:30

should see four distinct layers by

play09:33

grabbing three of those four layers and

play09:35

pulling them to one side you will create

play09:38

a three-dimensional cone out of the

play09:40

one's flat piece of filter paper that

play09:44

cone-shaped

play09:45

piece of filter paper will now fit very

play09:47

nicely into the funnel and you're ready

play09:49

to begin the process of filtration two

play09:54

more carefully pour the salt water into

play09:57

the funnel containing the filter paper

play09:59

we're going to use a technique that's

play10:01

called decanting notice that I'm holding

play10:04

a glass rod against the top of the

play10:07

beaker the rod will serve as a conduit

play10:10

for the flow of water so I can aim that

play10:13

flow of water directly where I want it

play10:16

as I tip the beaker and the glass rod

play10:19

over the funnel with the filter paper

play10:22

the salt water will flow down the

play10:25

surface of the glass rod and end up in

play10:28

the middle of the filter paper this will

play10:31

keep me from spilling salt water on the

play10:33

lab table

play10:35

our goal is to eventually transfer all

play10:38

of the sand into the filter paper in

play10:40

order to complete this task you may need

play10:43

to rinse the beaker several times with

play10:46

small volumes of water from the

play10:48

deionized water squirt bottle another

play10:52

successful filtration procedure

play10:55

completed notice that all of the sand

play10:57

has been transferred from the beaker

play10:59

into the filter paper and all of the

play11:02

salt water has flowed through the filter

play11:05

paper and is now in the beaker resting

play11:08

on the surface of the lab table be sure

play11:10

to show your teacher the completion of

play11:12

this technique before moving on the

play11:17

final technique that we will talk about

play11:18

today is properly lighting and adjusting

play11:22

a Bunsen burner of all the pieces of

play11:24

equipment in a chemistry laboratory

play11:26

perhaps nothing is more symbolic than

play11:29

the Bunsen burner in itself one of the

play11:34

biggest considerations when using a

play11:36

Bunsen burner in a laboratory is safety

play11:38

so at this point you should prepare your

play11:41

lab table area by removing any

play11:43

combustible materials any long sleeves

play11:47

or loose clothing should be rolled up or

play11:49

secured and dangling jewelry should be

play11:51

removed before attempting to light the

play11:54

Bunsen burner you should also put on an

play11:56

apron and safety goggles on the bottom

play12:01

of the Bunsen burner itself there is a

play12:03

gas shutoff valve before hooking the

play12:06

Bunsen burner up to the gas jet at your

play12:09

table please be sure to turn this valve

play12:11

off by turning it in the clockwise

play12:14

direction until it's tight in addition

play12:19

to closing the gas valve on the bottom

play12:21

of the Bunsen burner you should also

play12:23

adjust the combustion column downward by

play12:26

turning it in a clockwise direction this

play12:29

will prevent there from being too much

play12:31

oxygen mixing with the gas when you

play12:33

first light the burner once those two

play12:38

adjustments have been made on the Bunsen

play12:40

burner itself you may hook up the tube

play12:43

from the Bunsen burner to the gas jet at

play12:46

your table please be sure that they

play12:48

connect

play12:48

you know the rubber to to the outlet of

play12:51

the gas jet is tight at this point

play12:55

you're ready to turn on the gas at the

play12:57

table

play12:58

notice that the valve has been moved

play13:01

from a perpendicular position to a

play13:04

position that's parallel and pointing in

play13:06

the same direction as the outlet of the

play13:08

gas jet at this point no gas is coming

play13:12

through the Bunsen burner because the

play13:14

valve on the bottom of the Bunsen burner

play13:16

is shut off before attempting to light

play13:21

the Bunsen burner you should be sure

play13:23

that you can make a spark consistently

play13:25

with the striker that's provided when

play13:28

you can do this open up the valve on the

play13:31

bottom of the Bunsen burner to allow gas

play13:33

to move up through the combustion

play13:35

chamber make a spark with a striker and

play13:38

the Bunsen burner should light notice at

play13:41

this point that the flame of the Bunsen

play13:43

burner is a yellow color because not

play13:45

much oxygen is mixing with the gas to

play13:49

increase the amount of oxygen and the

play13:51

temperature of the flame simply turn the

play13:54

combustion column in a counterclockwise

play13:56

direction moving it upward away from the

play13:59

base this will increase the amount of

play14:02

oxygen that mixes with the gas and

play14:04

caused the flame to turn a bright blue

play14:06

color

play14:08

notice that the flame has a brighter

play14:10

blue inner cone the tip of that inner

play14:13

cone is the hottest most part of the

play14:16

Bunsen burner flame be sure to show your

play14:20

teacher that you can properly light and

play14:22

adjust the Bunsen burner and ask them if

play14:25

time allows if you can evaporate the

play14:28

water from the salt water mixture that

play14:30

we filtered previously which would

play14:33

separate the salt from the water itself

play14:37

if time doesn't allow simply clean up

play14:40

your equipment and put it away at this

play14:42

time congratulations you have

play14:44

successfully completed the practicing

play14:47

laboratory techniques lab

Rate This
β˜…
β˜…
β˜…
β˜…
β˜…

5.0 / 5 (0 votes)

Related Tags
Chemistry LabLab TechniquesElectronic BalanceMeasuring MassVolume MeasurementGraduated CylinderTemperature ReadingFiltration ProcessBunsen BurnerLab SafetyExperimentation