Sample Collection & transportation
Summary
TLDRThis video script is a comprehensive guide to sample collection and transportation, focusing on universal precautions in healthcare settings. It covers the importance of barrier protection, glove use, and proper labeling for various clinical specimens. Detailed instructions are provided for collecting samples like abscess, blood, urine, stool, and respiratory secretions, emphasizing aseptic techniques and transport conditions. The script also discusses potential issues like mismatched information, temperature mishandling, and sample contamination, offering insights into laboratory diagnostics and patient care.
Takeaways
- π· Universal precautions are crucial for healthcare workers collecting samples, including using barrier protection, gloves, and face masks or goggles.
- π©Ί When handling body fluids or sharp objects, it's important to use caution to prevent accidental needle pricks or injuries.
- π§Ό Cleaning work areas with soap and water or disinfectants after sample collection helps maintain a sterile environment.
- π₯ Proper sample labeling is essential, including patient details, specimen type, collection site, and unique ID numbers for accurate identification.
- π The requisition form is vital for sample submission, as it provides patient history and clinical information necessary for accurate diagnosis.
- π« Mismatched information on labels or requisition forms can lead to sample rejection, emphasizing the need for accuracy.
- βοΈ Maintaining proper transport temperature for samples is critical, as improper temperatures can affect sample integrity.
- β±οΈ Timely transportation of samples within specified time frames is necessary to ensure sample viability and accuracy of results.
- π¬ The quantity and quality of samples are important; insufficient or contaminated samples may be rejected and require recollection.
- π©Έ Specific techniques are required for collecting different types of samples, such as abscess, blood, body fluids, and urine, each with unique considerations for sterility and transport.
Q & A
What are universal precautions that should be taken while collecting samples?
-Universal precautions include using barrier protection, wearing gloves when handling body fluids, wearing a face mask or goggles, and being cautious when handling sharp objects like needles and syringes.
Why is it important to clean the work area and use disinfectants?
-Cleaning the work area and using disinfectants is crucial to prevent cross-contamination and ensure the accuracy of the samples collected.
What should be included in proper sample labeling?
-Proper sample labeling should include the patient's name, clinical specimen type, the site where the sample was taken, a unique ID number, specimen type, date, time, and place of collection, and the initials of the person collecting the sample.
Why is the requisition form important for sample processing?
-The requisition form is important because it provides essential patient information and clinical history, which are necessary for accurate diagnosis and reporting.
What are the common types of body fluids that may require collection for microbiological analysis?
-Common types of body fluids for collection include blood, urine, ascitic fluid, pleural fluid, and pericardial fluid.
How should blood samples be collected for culture?
-Blood samples for culture should be collected using a sterile technique, with the appropriate volume depending on the patient's age and condition, and transported to the laboratory in specific culture bottles.
What are the different methods for urine sample collection and which is the most common?
-The different methods for urine sample collection include clean-catch midstream, catheterization, and suprapubic aspiration. The most common method is clean-catch midstream urine.
Why is it important to avoid contamination when collecting stool samples?
-Contamination of stool samples can lead to false-positive results, so it's important to collect the sample in a sterile container and avoid contact with disinfectants or the toilet environment.
What are the criteria for rejecting a sample in microbiology?
-Samples may be rejected if there is a mismatch of information on the label and request form, improper transport temperature, delay in transportation, insufficient quantity, leakage, or if collected without a requisition form.
How should samples be transported to ensure their integrity?
-Samples should be transported promptly to the laboratory using appropriate containers and transport media, and kept at the correct temperature as specified for the type of sample.
Outlines
π§ͺ Laboratory Sample Collection and Precautions
The speaker from Aaron or Mike Ability Department introduces the topic of sample collection, emphasizing the importance of universal precautions in healthcare settings, particularly in hospitals. Key points include the use of barrier protection, gloves when handling body fluids, and face masks. Caution is advised when handling sharp objects to prevent needle pricks. The speaker also highlights the need for proper cleaning of work areas and the importance of sample labeling with patient details, specimen type, collection site, and a unique ID number. The necessity of a requisition form for accurate diagnosis and reporting is also discussed, along with the importance of patient history and correct sample labeling to avoid mismatches and ensure proper sample handling and transportation.
π©Ί Proper Sample Collection Techniques
This section delves into the specifics of collecting various types of samples, such as abscesses, pus, blood, and body fluids. The speaker explains the differences between aerobic and anaerobic samples and the importance of collecting samples from the correct sites. For abscesses, the necessity of cleaning the infected area with sterile saline or alcohol before collection is emphasized. When collecting pus, the use of syringes for aspiration is recommended. For blood samples, the speaker details the types of blood culture bottles available and the appropriate volumes of blood to be collected based on the patient's condition. The collection of body fluids like ascitic, synovial, pericardial, and pleural fluids is also discussed, with a focus on using aseptic techniques and proper transport within a specified time frame to ensure sample integrity.
π‘οΈ Urine and Stool Sample Collection
The speaker discusses the collection of urine and stool samples, highlighting the importance of clean catch midstream urine and the different methods for collecting urine, including catheterization and suprapubic aspiration. The need for proper patient instruction and aseptic technique is emphasized to avoid contamination. For stool samples, the speaker advises on the use of a clean, leak-proof container and the appropriate amount of sample to be collected. The importance of transporting samples promptly to prevent degradation is also covered, with specific instructions for handling liquid and formed stool samples.
𧫠Specialized Sample Collection: Stool, Gastric Aspirate, and Sputum
This part of the script focuses on the collection of specialized samples such as stool, gastric aspirate, and sputum. The speaker provides guidance on the appropriate containers and transport media for stool samples, with an emphasis on avoiding preservatives that can interfere with certain tests. For gastric aspirate samples, used primarily for TB detection, the speaker outlines the need for aseptic conditions and the use of a nasogastric tube. The collection of sputum samples is also discussed, with instructions on how to induce expectoration and the importance of avoiding saliva contamination. The speaker stresses the need for proper patient preparation and the use of hypertonic solutions to promote secretion for sputum induction.
π¬ Nasal, Pharyngeal, and Eye Sample Collections
The speaker addresses the collection of samples from the nasal, pharyngeal, and eye areas. For nasal samples, the use of polyester swabs and a gentle rotation technique is recommended. Pharyngeal samples require aseptic swabs and a specific suction technique. Eye samples should be collected under aseptic conditions and transported promptly to the laboratory. The speaker also covers the importance of proper patient positioning and the use of appropriate materials to prevent contamination and ensure accurate sample collection.
π₯ Final Remarks on Sample Collection and Transportation
In the concluding part, the speaker summarizes the importance of proper sample collection and transportation, emphasizing the need for aseptic techniques and adherence to guidelines to ensure sample integrity. The speaker invites any further questions and provides contact information for additional clarification, highlighting the importance of accurate laboratory testing in patient diagnosis and treatment.
Mindmap
Keywords
π‘Universal Precautions
π‘Sample Collection
π‘Transport Medium
π‘Labeling
π‘Requisition Form
π‘Sterile Technique
π‘Disinfectant
π‘Specimen
π‘Microbiology Department
π‘Contamination
π‘Aerobic and Anaerobic Cultures
Highlights
Importance of universal precautions in sample collection and transportation.
Use of barrier protection and gloves to handle body fluids.
Necessity of face masks or goggles while collecting samples.
Caution while handling sharp objects like needles and syringes.
Proper disposal of condom native materials using biohazardous waste procedures.
Cleaning work areas with soap and water or disinfectants.
Thorough hand washing after handling samples.
Essential aspects of proper sample labeling for microbiology departments.
Requirements for patient name, clinical specimen, and unique ID number on labels.
Importance of requisition forms for accurate diagnosis and reporting.
Criteria for sample rejection due to mislabeling or improper transport.
Transport temperature requirements for different types of samples.
Avoiding delays in transportation and the impact on sample integrity.
Correct collection techniques for abscess samples, including swab and aspirate methods.
Instructions for collecting blood samples, including quantities and sterility requirements.
Different types of blood culture bottles and their appropriate uses.
Collection and transport guidelines for body fluids like ascitic and pleural fluid.
Urine sample collection methods, including clean catch, catheter, and suprapubic aspiration.
Proper handling and transport of stool samples for bacterial and parasitic analysis.
Collection techniques for sputum and gastric aspirate samples for TB detection.
Guidelines for collecting nasopharyngeal and oropharyngeal samples.
Contraindications and precautions for sample collection to avoid contamination and errors.
Final remarks on ensuring sample quality and accuracy in lab testing.
Transcripts
good afternoon everyone
what I am going to be discussed sample
collection and transportation
I am from Aaron or Mike ability
department tutor before going to be
topic we should know something about in
yourself precautions without any
universal precautions we don't go for
any other work mostly in hospitals okay
and what are universal precautions you
should take while collecting samples
first one is use barrier protection in
case of any work but once in working
area please cover that whopper notes by
using some other it reads like some
sterile materials next you should be
some gloves while handling body fluids
or some other condom metal materials
next you should be at some face mask or
gone without wearing any face masks are
gone you never take any sample and use
caution because while angling sharp
objects like needles and syringes or
some other thing okay you should take
some cautions because it should be taken
some needles needles pricking during
next discover condom native materials by
using by Assad miss are by or Sur
procedures next clean work area by using
some soap and water
if soap on what is not available then
use disinfectant then disinfectants not
available in Italy called respective
fellow to come and clean the respect to
areas then wash were and thoroughly its
soap and water after what gives you over
again each and after everything is over
then wash were close by using hot water
don't wash your clothes by using some
chilled water or ordinary water okay
before correcting sample labeling is
very important without proper labeling
you don't send any sample to micro micro
budget Department okay
then coming to labeling patient name is
very important we should write patient
improperly in bold letters don't write
any some giant letters then write
clinical specimen Part F of clinical
specimen whether it is in blood sample
whether it is an urine sample whether it
isn't per sample whatever we should
mention clearly then what site where you
an example whether you take sample from
left hand or right hand or lift lift
trick or like a right leg whatever you
shipment you should mention properly
then unique ID number in hospital each
and every patient a unique ID number is
there without any unique ID number you
don't send any sample to my committee
department then pessimal type you serve
what type of specimen then date time and
place of collection and name an initial
of character on what whether question is
ICU or OPD what operation Tector
whatever which should be mentioned
properly then we coming to requisition
form it is very important
without requisition form don't send any
sample to microbiology department based
on patient clinical esteem only we have
to diagnose we have to give accurate
report ok in that requisition form we
should fill all the information ok this
patient history is very important
without patient history don't send any
sample to Micro Department and signature
of consultant that is very important and
sign of the sample character who is
going to be correct sample and date and
time patient name patient ID age 6 word
sample or site and test request what
tests we want to share form ok these are
some criteria for inserting samples
which would remember all those things
while collecting samples too much and
sending to make up my geometry
department okay first you should know
mismatch of information on the label
under request this is very very
important because sometimes what
happened in that container should be
some other day our patient name and
request should be some other patient
name ok this should be avoided
next in a perfect transport temperature
sometimes in some blood blood sample is
in 4 degree Celsius
let them be using some ice bag in some
in some sample circuit 37 degree Celsius
you use by some other appropriate
technique in some sample using room
temperature is no problem you have to
same sample by room temperature then
access the delay in transportation
notice in all the sample it will 2 hours
if going about 2 hours they are not
responsible in appropriate transport
medium
suppose element dissident fixating agent
or pessimal should be in the right
condition our sample should be some
questionable questionable only we don't
know what type of sample that everything
should be rejected an insufficient
quantity in CSF okay no problem we have
to take 0.5 to 1 ml in your example you
don't send 0.5 to 1 ml at least we sent
5 ml of sample then leakage that is very
important in case of any leakage we
never accepting that sample then without
any requisition form see by using this
book transfer a specimen then we move to
one by one how to collect sample that
how to transport and all we are to
reserve first - abscess in abscess means
the tribulation own post fuels of ulcers
ok
the abscess should be two types aerobic
and anaerobic in arabic may be
superficial I never make me say it
should be depletions ok before
collecting sample we should wipe with
sterile saline alcohol the infected area
then go to take swaps rub along the edge
of the hole after taking sample we
should be transport within 2 hours and
we require 2 straps if we send one swabs
on recent of the three of us it should
be rejected in case of only one step ok
then you immediately call to my cabbages
you have to explain the condition of the
patient then when we accept other ways
me never accept any samples here see ya
based on fashionista money we give
reports in case of post-operative
patient conscious significant
without any patient history or we give
report they ought to give only no growth
next coming to pus
but mostly post should be aspirated by
using syringes only in case of closer
space abscess
what happened which would thicken
ammonite skin by using sterile saline or
alcohol then insert needle and aspirate
the first part of a sufficient amount
you take and send it to my compulsion
Department in case of open wound
remove superficial exudate okay aspect
through marginalist margin while you
sings sir ng or if there is no purse we
can take swaps and yo to rub it and send
it to my cabinet department see this is
an expected purse I'm coming to blood
sample in blood sample my cables you
mostly using for culture only and next
thing is serves a reactions me that
antigen antibody reactions I'm coming to
culture in our department some that
touch a bottle is available that is
especially suitable for culturing
bacteria bacteria swoon living okay in
the blood culture bottle we have three
types of bottles available in our
department one is arabic bottle another
big bottle and periodic bottle you come
and ask my culture department we want to
we want here a big bottle we want an
anaerobic bottle periodic but whatever
you want you come at nasse my comedy
department we will provide in case of in
fact you should take 0.5 to 2 ml in case
of children you take 2 to 5 ml in case
of forward you take 5 to 10 ml ok this
blood should be taken by wind puncher
only I'm coming to depression of in
puncher first you have to look at skin
you have to pail plate whether it's
serving appropriately then use alcohol
for 5 minutes then play for 30 to 60
seconds then take tension of IOD you
have to apply to scheme again right for
45 to 60 seconds then without poly
plating okay you have to take 20 ml of
blood and of this 20 ml 10 ml enacted
into arabic bottle aunt animal should be
knocked at end in a big bottle this is
based on a patient condition one day you
have to take in case of Petula's very
sick when you take 5 ml okay so question
listen LT then you take 20 ml then this
was our ng
in shop container label bottle and send
it to laboratory see these are metal
check bottles in case of travel febrile
episode you take two set of two sets of
blood work in case of battle
endocarditis you take three sets of
blood cultures bottles
okay don't take any three sets of blood
within 24 hours
then come into body fluids there are so
many body fields available okay of this
only few body flicks I mentioned here
okay on it somatic field ascetic fate by
signing off a pericardial fluid pleural
fluid and see yourself if you want to
take in your body fluids same thing
first we apply disinfectant or sterile
saline
then insert needle then you have to take
aspirate how much amount you want to
take we want 5 to 10 ml you take 5 to 10
ml if you want 10 to 20 ml you take 10
to 20 ml how much amount you want you
take then immediately transfer into
sterile screwcap container if this crew
captain is not available then you die to
inducted into periodic blood culture
bottle system now label it properly and
transport within 15 minutes we send
after 30 minutes we are not responsible
you know to sustain sample again back to
respective words see this is sterile
container may be some needle escalators
I'm coming to urine sample collection of
urine sample should be very important
because sometimes what happens some
cross combination will take place okay
urine collection must be three types
first one is clean catch midstream urine
Nixon catheter is green that means
suprapubic expression urine you see one
by one first one is clean catch
midstream urine okay it is very
important because most of a time using
clean catch midstream urine monthly okay
instruction to patient is very important
instruction to patient clean period the
area with soap and water then tell
inform the patient to pass initial urine
into toilet then collect midstream urine
into sterile cup suppose the cup the
holding cup pass on should be down or
you have to touch in select cup okay Oh
to inform immediately to respect to
nursing stuff tell them to take another
new cup okay before collection label is
important because you are incurring what
happens sometimes it should be spoiled
entire continent during passing urine so
you know to avoid all those things
here to the label all those things
properly before collecting any samples
okay if we found more than three or four
types of bacteria okay what happened the
same ultimately we put into the
contamination
it's kindly simply paid sample okay
suppose inhibited sample also again
three to four bacterias then we give
significant growth see this is an
pictorial representation of how to
collect urine sample see was clean ever
pee with a little soap and water then
was initially urine into toilet
then you collect middle portion of the
urine okay then cross it properly then
label it and send it to laboratory now
coming to catheterize via urine okay it
is very important this was mostly useful
for patient we having in under like some
unrelated disease condition or some
children you have to use
mischaracterized urine okay
first they inform to clean the brain
Oracle AERS open water then apply some
sprayed or some sterile saline to
decontaminate then insert catheter into
bladder then discard initial URI then
collect specimens towel cup okay this is
for normal catheter during incoming to
chronic in Velenje catheter work in that
what happened you can take sample from
junction between kettle and collection
to you don't take any sample from this
discarding back okay if you come collect
urine from discoloring back mostly from
contamination so only because it is
24-hour collection what happened during
24 hours collection
mostly fuel and fuel contamination
smoothly so you don't track any sample
from this discarding back it should
collect sample from this junction
modeling then coming to suprapubic
aspiration sample this is mostly from
some unrelated business condition only
mostly in periodic patient okay those
who are unable to pass urine
okay what happened you have to follow
the full aseptic condition because they
are to insert needle into super pubic
region okay it should be little risk in
from the patient bladder should be
filled before performing this procedure
then papers came with alcohol or iodine
then give some little key for local
system then insert needle to drop and
two ml e 0.2 centimeters of the above
the synthesis then a spec 20 ml of urine
for culture then send it to laboratory
see this is a procedure how to take
urine by using super epic aspirations
then coming to scroll sample stool is
generally non sterile sample okay before
cutting stool sample we should know
something about stool okay what is a
normal floor of elementary cannot they
mainly we give reports without knowing
elementary can all normal flora we never
give any reports okay freshly possible
sample is very important avoid basement
from Big Bang bed pan is not a suitable
placement for culturing stool sample use
sterile or clean container okay
don't use any container with
disinfectant during outbreak you should
correct
10 to 20 percent stool samples I'm
coming to collection we take clean
leak-proof container in case of liquid
liquid you take one tablespoon that is 5
ml of stool sample in case of Foreman
stool you take pea-sized rule is
sufficient then after collecting the
transport within 30 minutes
if delay transfer fecal sample into
transport medium
don't send any sample for or don't send
any sample with preservative or
something say tubes if we send any
samples in preservatives or fixatives it
is not suitable for right Widman
examination president difficile toxin
reduction reduction of viruses elias on
lattice occupation tasters and coming to
bacterial culture mostly we encounter
Salmonella Shigella and Campylobacter if
doctor asking if specify specifying we
want be Brio we want aroma last report
we want to BZ amana's el Senor equal
o157 hs7
then that time only ought to process
otherwise we never possess this thing
suppose if doctor asking she got ox and
I say then you follow otherwise you
don't follow any Shiga toxin I see ok
this everything
should be recommended by CDC Center for
Disease Control suppose some people
giving report equally in that thing
and can't you go and confirm in doctors
what is a patient condition then we will
put as an equalising significant and
don't go don't give any grand
possibility basically a gram positive
cocci and coming to school for parasites
okay now would it take three perceptual
sample to one product we celebrate you
mostly using passivity here ten person
is firmly or Paul evening chloride okay
this unprecedented action and PCR this
present sample is not suitable for PCR
piece here and antigen detection and can
come in two vectors where this is mostly
suitable for small children and nibbled
at a patient and other situation
suggests those who unable to take it
from the bid that time you have to take
this rectal swab come into collection in
cells where approximately two point five
centimeter IELTS printer and figures
should be visible on the sweats they
know to take out immediately and
transport immediately without any delay
delay use the transport medium it is not
suitable for Paris le Direction talks in
toxic direction and my agents it should
be for only for culture smoothly Bechdel
culture only and coming to draw back
there is no microscopy assessment
possible unless material available on
out a chimera for viruses and coming to
gastric aspirate the gastric content
mostly useful for TB detection mostly
what happened some in children have
difficulty to expediting put them simple
so that time what happened we were to
recommend gastric has spread because
what happen in gastric the spread and
children with pneumonia sleep what
happened during Miko's Larry mechanism
okay the entire milk is sample what
happen comes into a participatory track
when reaches into throat region again
the swallowing the children then what
happened is a intestine to stomach
region okay that is enforced during
stomach region what happy contain which
source of TB TB organisms and come into
collection before collection instruction
is very important
in section 2 a patient or come early
morning an infirmity patient you should
sleep at least six hours before
collection okay and infirmity patients
should not eat or drink anything
overnight to prevent the stomach from
empty and come collection by using an
ass or gastric true okay
don't use any sub W because it is in
vector static it will kill all bacterias
and mostly rig you report going for
negative only use large bore nasogastric
tube don't do small sized strips okay
and white too deep because what happen
if we entered too deep and this on the
fire is raging okay what happen it will
take whatever condense into fire season
and you have to take from outside so it
leaving some false positive or false
negative a put during processing should
be too simple swim required for
processing one non each day suppose if
it is not possible in form to respect to
my cable this I mean from my shiny
condition is very difficult so it is not
possible to take two sample one is
sufficient of after taking sample you
have to transfer 5 to 10 ml gastric
fluid from syringe into sterile
container then add equal volume of
sodium bichromate solution because in
what happened acidic we ought to take in
sample from gastric content in gastric
content what happening because
increasing in pH increasing acidic pH so
neutralizes acidic pH by adding sodium
bicarbonate solution to prevent be the
section of table food basically
I am coming to spot em sample protein
sample is mostly taken by using two
methods one is induces put'em next one
is export a test bottom and coming to
export at this bottom it is very very
important most of the time a of you take
on coming to elaborate it is put on only
and this is a very risky also because
most of the time patient will come and
meet with saliva also so it requires
some initial technique and what happened
some good knowledge about patient and
nurses then we only be getting this
Goods put them and come into collection
we before collection it should be
informed to patient don't take any food
one two tours before taking any sample
ok mode should be rinsing with soap
saline or water and should instead to
patient to provide beef cuff ok and
weapon the cuttin L and keep it near to
the mote okay what happened doing diff
cover depends put him back Lee
come on contact with wide monster
container then immediately close that
tube and market market market properly
and send it to a laboratory many may
avoid avoid cyber contamination
suppose he have any saliva condemnation
it's it's not suitable for my ecology
laboratory we send it back to respect to
love what's see these are common
procedure cleaned my mouth and breathe
in and out three times then take a
sample then come into a certain area it
is a rejection criteria first put them
sample suppose if sample is sending to
microbiology laboratory s and remorse
saliva e and microscopy out to find out
more than ten squamous epithelial cell
it is rejected okay
come into excellent sample it should be
more than twenty five polymorphonuclear
cells plus few epithelial cells that is
five to six cells it is it is excellent
suppose if you find out more than ten
squamous epithelial cell on one or two
per cells it is not suitable for culture
it may be some saliva contaminations
okay and come in to induce report on
okay it is mostly suitable for patient
unable to produce bottom sample because
mostly it's the patient only it is
difficult to do in this put'em okay but
in that time are using some some partial
technique to take sport and sample okay
before collection patient should be
brushed the buccal mucosa
tank and gums and queen you the patient
some nibblies hypotonic solution what
happened this solution it will give for
the area secretion and promote the
coughing and allowing butum spittle
secretion to outside
I am coming to contraindications and
precautions while collecting samples
because induces put em be giving
hypertonic solutions only what happened
15 giving iPod on excellent solution
it causes Branco construction did this
manga construction what happened
perchance is going to be some critical
stage okay
before giving this iPod on external
solution in case of asthma patient or
some suspect asthma patient a civil lung
impairment function patient able to use
a medical premedication then mainly we
give you this Austral solution otherwise
I never give any sterile solution then
apart from this in case of severe
cufflink pressure don't form this
procedure okay
an apart from this patient young Emma
Topsy's accurate expedited stress
unstable cardiovascular acts and
abdominal a cerebellar infections and I
fucsia
run functional impairment pneumothorax
pulmonary emboli these are conditions we
never use this technique we go for
experts put and technique only I'm
coming to it is equation endotracheal
take secretion okay it mostly by you
that custom a patient mentally what
happened by using glue can trap you can
take sample okay what happened mostly
Tyco so my patient
mostly GN are present in this our mouth
region and apart from this this stack of
straw me equipments okay
it is not clinically revelant okay
sometimes what happen it will enters
into your lungs and cause pneumonia
during aspiration okay we have to
confirm the patient history and based on
the semi quality report we have to
correlate and new clinic miss on a semi
quality culture report on clinical
condition of the patient then we only be
out doing new clinic it is significant
other way see never give it is not
significant and coming to NASA
pharyngeal aspirate
okay this is also another is Polly
secretions what happened attach Amica
strapped into suction out like pitously
this is some suction tip okay we have to
attach Inlet and outlet okay in outlet
what happen inside into NASA pharyngeal
region in outlet which would connect
into some suction pressure okay with the
help of general section what happened it
will take all week a sample from a
prospective tract into this we can strap
this tube then after taking this thing
you have to cut this tube and
immediately transferred into laboratory
you have to transport within 15 minutes
if taken more than Tatem 30 minutes what
happened it should be condemnation only
so that time you have to reject sample
so you should take care while
transporting sample also now to place
the sampling ice box or in refrigerator
it's transportation delay and coming to
NASA fragile aspirate now NSF orange
else perhaps okay
infirm to a patient sit comfortably in
chair or some other our stool then tilt
helped backwards and insert flexible
finds shaft Polly stress wraps don't use
any cotton swabs okay if you use cotton
swabs in case of anaerobic
we do some wrong reports use some
polyester swabs into nostrils and back
to the nasa pharynx leave it for few
seconds and withdraw slowly by using
gentle rotation and coming to ear sample
in air sample in case of in air sample
over to clean in a year with soap and
water before functioning haradrim
okay then after washing you have to
puncture a spread material okay then
in case of is no pus then you're to take
swabs and go to Gen gentle rotate and
send it to laboratory in case of outer
air rotor it takes trial cotton swabs it
should be most units saline awesome
transport media and how to wipe the
crust with sterile saline monthly then
you have to gently rotate the swept into
outer cannot then you take gently
outside then transported to laboratory
immediately I am coming to ice samples
mostly using conductive also apps it res
fluids on corner scraping only this we
require some full aseptic conditions
aren't fully precautions without using
any precaution don't take any eye
samples those who express that represent
only taking this samples okay after
taking this sample transport within 50
minutes to laboratory if you delay don't
send any sample to laboratory otherwise
you keep it in refrigerator then we same
sample after 30 minutes to one another
whatever but before whenever you send
sample to a laboratory if you have any
clarification okay see this website okay
in this episode of 114 videos regarding
about sample collection and lab
management system ok if you have further
any doubts come and ask me in Department
thank you
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