How to Use an Analytical Balance

Summary

This script offers a detailed guide on using an analytical balance in biotech labs for precise measurements up to 0.0001g. It emphasizes the importance of a steady surface, avoiding air currents, and proper calibration. The video demonstrates measuring sodium bicarbonate on weighing paper and powdered Tris buffer in a weigh boat, highlighting the process of taring, adding substances, and documenting results. It concludes with cleaning procedures and resources for further learning in life sciences.

Takeaways

• 🔬 Analytical balances are essential in biotech labs for precise measurements up to four decimal places.
• 📏 A paperclip weighs approximately one gram, indicating the balance's sensitivity for small weights.
• 🚫 Analytical balances are not designed for heavy items; the example balance has a 310-gram limit.
• 🏗️ Place the balance on a steady surface like a marble counter to minimize vibrations.
• 🚫 Avoid using the balance near doorways or air vents due to its sensitivity to air currents.
• 🔄 Check and adjust the balance's level using the air bubble within the black ring.
• ⏱ Allow the balance to warm up to reach the required operating temperature if it's been unplugged or turned off.
• 📝 Use weighing paper for items under 5 grams and follow specific steps to zero out the paper's weight.
• 🧪 For heavier items, use a weigh boat and aim for accuracy within plus or minus 0.02 grams.
• 📋 Always document measurements and procedures in a lab notebook for record-keeping.
• 🧼 Cleaning the balance is crucial, especially at the end of the day; follow proper steps to maintain its accuracy.

Q & A

What is an analytical balance and what is its primary use in a biotech laboratory?

An analytical balance is a piece of equipment used for taking very precise measurements, accurate to four places to the right of the decimal point. It is primarily used in biotech laboratories for weighing small to moderately heavy items with high accuracy.

Why is it important to consider the workspace when using an analytical balance?

The workspace is important because the balance needs to be placed on a steady surface to minimize vibrations. It should not be used near doorways or air vents to avoid being affected by air currents, which can impact the accuracy of the measurements.

What is the maximum weight capacity of the analytical balance mentioned in the script?

The analytical balance mentioned in the script has a maximum weight capacity of 310 grams, which is comparable to the weight of a can of tomato soup.

How should the balance be checked for level before use?

To check if the balance is level, you should look at the air bubble and adjust the feet until the bubble is inside the black ring.

What should be done if the balance has been unplugged or turned off?

If the balance has been unplugged or turned off, it needs to warm up and reach the required operating temperature before use.

Why is weighing paper typically used for measuring less than 5 grams?

Weighing paper is used for measuring less than 5 grams because it helps to prevent contamination and provides a stable surface for the substance being weighed, ensuring accuracy.

How should you prepare the weighing paper before placing it on the balance pan?

To prepare the weighing paper, fold the opposite corners together and make a crease, then repeat for the other corners. This helps to create a stable platform for the substance on the balance pan.

What is the purpose of pressing the tare button on the balance?

Pressing the tare button on the balance zeros out the weight of the weighing paper or container, allowing the balance to measure only the weight of the substance added to it.

Why is it important to document the measurements taken with the analytical balance?

Documenting the measurements is crucial for maintaining an accurate record of the experiment, which can be referenced for future analysis and to ensure the reproducibility of the results.

How should the analytical balance be cleaned at the end of the day?

To clean the balance, put it into standby mode or unplug it, remove the weigh pan and components, use a soft bristle paintbrush to sweep off any powder, and then replace the drip ring and weigh pan.

Where can one find a life science course to learn more about using an analytical balance?

To find a life science course, one can visit nccommunitycolleges.edu or ncbionetwork.org for more information.