Unilateral Ureteric Obstruction: Reversible and Irreversible Model | Protocol Preview

Summary

This video demonstrates a mouse model of obstructive neuropathy induced by unilateral ureteric obstruction, which can either be irreversible or reversible. The model involves ligating the ureter to create hydronephrosis, with a procedure for reversible obstruction using soft plastic tubing. Over time, the kidney becomes hydronephrotic, and in the reversible model, the obstruction is reversed to allow urine flow, leading to kidney decompression. This model is used to study tissue injury, fibrosis, and remodeling after obstructive nephropathy, with experiments comparing outcomes after seven days of obstruction and subsequent reversal or sacrifice of the mice.

Takeaways

• 😀 The video presents a mouse model for studying obstructive neuropathy induced by unilateral ureteric obstruction (UUO).
• 😀 The obstruction can either be irreversible or reversible, with histological data showing structural injury and recovery.
• 😀 A schematic cartoon illustrates the major steps involved in the model, focusing on the left kidney, ureter, and bladder.
• 😀 A standard unilateral ureteric obstruction is induced by ligating the ureter, causing hydronephrosis in the kidney over time.
• 😀 To study reversible obstruction, a soft plastic tubing is applied around the ureter, limiting excessive dilation during obstruction.
• 😀 After seven days of obstruction, the soft tubing is removed, and the ureter is severed above the ligation site for reversal.
• 😀 The unobstructed ureter is anastomosed into the bladder to allow urine flow, and the kidney decompresses over several days.
• 😀 The UUO model is used to study tissue injury and fibrosis resulting from obstructive nephropathy.
• 😀 The reversible UUO model (rUUO) is employed to investigate tissue remodeling and recovery following injury.
• 😀 A typical experiment involves inducing UUO on Day Zero, with mice either being sacrificed or monitored after seven days.

Q & A

What is the main focus of the video?

The video focuses on a mouse model of obstructive neuropathy induced by unilateral ureteric obstruction, detailing the histological data of structural injury and its recovery following reversal.

What does the schematic cartoon in the video illustrate?

The schematic cartoon illustrates the major steps of the unilateral ureteric obstruction model, showing the left kidney, ureter, and bladder, as well as the process of obstruction and recovery.

How is a standard unilateral ureteric obstruction induced in the mouse model?

A standard unilateral ureteric obstruction is induced by ligating the ureter, which causes the kidney to become hydronephrotic over time.

What is the purpose of using soft wall plastic tubing in the reversible ureteric obstruction model?

The soft wall plastic tubing is applied around the ureter at the point of obstruction to limit excessive dilation of the ureter during the reversible obstruction process.

What happens to the kidney in the reversible unilateral ureteric obstruction model?

In this model, the kidney becomes hydronephrotic over time due to the obstruction. Once the obstruction is reversed, the kidney decompresses over several days.

How is the ureter treated in the reversible model once the obstruction is reversed?

Once the obstruction is reversed, the soft tubing is removed, and the ureter is severed just above the ligation level. The unobstructed ureter is then anastomosed into the bladder to allow urine flow.

What is the significance of the unilateral ureteric obstruction (UUO) model?

The UUO model is used to study the development of tissue injury and fibrosis following obstructive nephropathy.

What is the role of the reversible ureteric obstruction (RUUO) model?

The RUUO model is used to study tissue remodeling that may follow injury and recovery after the obstruction is reversed.

What is the typical experimental procedure involving both UUO and RUUO models?

In the typical procedure, mice undergo UUO on Day 0, and after seven days, they are either sacrificed or used for further studies involving tissue analysis.

How long is the ureter obstructed before being reversed in the RUUO model?

The ureter is obstructed for seven days before the reversal procedure takes place.