PEMERIKSAAN CREATININ

Summary

In this video, the process of creatinine testing without deproteinization is demonstrated. A serum sample from a 63-year-old female patient is used, along with reagents R1 and R2. The preparation involves creating a monoreagent, which must be used within five hours for stability. The testing process includes pipetting the monoreagent into cuvettes, followed by adding distilled water and the serum sample. After homogenizing the sample, the test is conducted using a kinetic measurement method with two absorbance readings. The final result, 1.7 mg/dL of creatinine, is provided along with a brief conclusion for the viewers.

Takeaways

• 😀 The demonstration focuses on creatinine testing using a method without deproteinization.
• 😀 The patient sample used is from a 63-year-old female.
• 😀 Reagents used for testing are labeled as R1 and R2.
• 😀 A monoreagent needs to be prepared and used within five hours for stability.
• 😀 A 500-microliter micropipette is used to transfer the monoreagent to the first cuvette for blank measurement.
• 😀 A 25-microliter micropipette is used to add aquabides to the first cuvette for the blank sample.
• 😀 The serum sample must be homogenized before being pipetted into the cuvette for analysis.
• 😀 After adding the serum sample, it is mixed using the machine's mix function.
• 😀 The machine settings are configured for creatinine testing using the 'fixed time' or 'kinetic' method, with two readings taken.
• 😀 The final result for creatinine is obtained without deproteinization, and in this case, the result is 1.7 mg/dL.

Q & A

What is the purpose of this demonstration?

The purpose of the demonstration is to show how to perform a creatinine test without deproteinization using specific reagents and equipment.

What type of sample is used in this creatinine test?

The sample used is a serum sample from a 63-year-old female patient.

Why is the monoreagent prepared, and what is its stability time?

The monoreagent is prepared to carry out the test. It is only stable for 5 hours after mixing, so it must be prepared fresh according to the needs of the test.

What reagents are used in the creatinine test?

The reagents used are R1 and R2, which are required for the creatinine test.

How is the monoreagent applied to the cuvettes during the test?

A micropipette with a 500-micron volume is used to pipette the monoreagent into cuvettes, starting with a blank cuvette.

What is the purpose of using aquabides in this test?

Aquabides is used in the first cuvette as a blank sample to help measure the background absorbance, allowing for accurate test readings.

How is the serum sample processed before testing?

The serum sample is homogenized by gently pipetting it into the cuvette, then mixed using a 'mix' button on the equipment to ensure uniform distribution before reading.

What settings are adjusted on the testing machine?

The machine is set to the appropriate testing method, which is a fixed time or kinetic measurement, with the sample and blank cuvette properly configured.

How long does the incubation step take in this creatinine test?

The incubation step lasts for 60 seconds before the first absorbance reading is taken.

What is the final result of this creatinine test?

The final result of the creatinine test, after processing the sample, is 1.7 mg/dL.