Praktikum Kimia Farmasi Analisis - KCKT

Summary

This video script outlines a pharmaceutical chemistry laboratory experiment focused on determining the content of sulfamethoxazole in tablet samples using high-performance liquid chromatography (HPLC). The procedure involves preparing standard solutions, including dissolving the active ingredient in methanol, and filtering samples for precision. The video details the steps of preparing the solutions, measuring sample consistency, and using specialized equipment like a 0.2μm membrane filter for filtration. It emphasizes the importance of accuracy and following set protocols for successful analysis.

Takeaways

• 😀 The demonstration covers the analysis of Sulfamethoxazole concentration in tablet form using liquid chromatography.
• 😀 Laboratory equipment required includes 10 mL, 25 mL, and 50 mL volumetric flasks, HPLC-grade methanol, syringes, and 0.20 μm membrane filters.
• 😀 The first step in the procedure involves preparing a stock solution of the active ingredient, Sulfamethoxazole, by dissolving it in methanol and diluting to the 10 mL mark.
• 😀 A primary stock solution with a concentration of 10,000 ppm is prepared, followed by the creation of a working solution at 1000 ppm for analysis.
• 😀 For sample preparation, tablets are carefully weighed (125 mg) and dissolved in HPLC-grade methanol, then filtered using Whatman 40 filter paper.
• 😀 The filtered sample is transferred to a 10 mL volumetric flask and further diluted with methanol to achieve a homogeneous solution.
• 😀 The sample solution is filtered again through a 0.20 μm membrane filter to remove any particulate matter before analysis.
• 😀 Proper labeling of the calibration and sample solutions is essential to track which is which during analysis.
• 😀 The process emphasizes the importance of accuracy and careful handling of samples to ensure reliable results.
• 😀 The demonstration concludes with the preparation of both calibration and sample solutions, ready for the next stage of analysis using liquid chromatography.

Q & A

What is the main objective of the pharmaceutical chemistry lab experiment described in the transcript?

The main objective is to determine the sulfamethoxazole content in tablet formulations using liquid chromatography.

Which method is used to analyze the sulfamethoxazole content in tablets?

The method used is liquid chromatography, specifically employing HPLC-grade methanol as the solvent.

What are the key materials required for preparing the stock solution in this experiment?

The key materials include a 10 mL, 25 mL, and 50 mL volumetric flask, HPLC-grade methanol, sulfamethoxazole sample, and a balance for precise measurement.

What is the purpose of preparing the stock solution in this lab procedure?

The purpose is to prepare a concentrated stock solution from which subsequent working solutions will be made for the analysis of tablet content.

How is the stock solution prepared in the lab?

The stock solution is prepared by accurately weighing a specific amount of sulfamethoxazole, dissolving it in methanol, and transferring it to a volumetric flask, adjusting the volume to the desired level.

What equipment is used for filtering the solutions during the experiment?

A 0.2 micron membrane filter is used to filter the solutions, ensuring the removal of any particulate matter before analysis.

What is the purpose of the filtration process in this experiment?

The filtration process ensures that any particles or impurities are removed from the solution, resulting in a clear and homogeneous sample for accurate analysis.

What is the role of the 'working solution' in the experiment?

The working solution is a diluted form of the stock solution used for analysis, allowing the quantification of sulfamethoxazole content in tablet samples.

Why is it necessary to weigh and prepare the tablet samples accurately in this experiment?

Accurate weighing of the tablet samples ensures the precision of the analysis, as the amount of active ingredient must be known for correct determination of sulfamethoxazole content.

What steps are involved in preparing the tablet sample for analysis?

The tablet is first weighed, then dissolved in methanol, filtered to remove impurities, and finally diluted to a known volume to create a sample solution for analysis.