Sample Collection & transportation

Summary

This video script is a comprehensive guide to sample collection and transportation, focusing on universal precautions in healthcare settings. It covers the importance of barrier protection, glove use, and proper labeling for various clinical specimens. Detailed instructions are provided for collecting samples like abscess, blood, urine, stool, and respiratory secretions, emphasizing aseptic techniques and transport conditions. The script also discusses potential issues like mismatched information, temperature mishandling, and sample contamination, offering insights into laboratory diagnostics and patient care.

Takeaways

• 😷 Universal precautions are crucial for healthcare workers collecting samples, including using barrier protection, gloves, and face masks or goggles.
• 🩺 When handling body fluids or sharp objects, it's important to use caution to prevent accidental needle pricks or injuries.
• 🧼 Cleaning work areas with soap and water or disinfectants after sample collection helps maintain a sterile environment.
• 🏥 Proper sample labeling is essential, including patient details, specimen type, collection site, and unique ID numbers for accurate identification.
• 📝 The requisition form is vital for sample submission, as it provides patient history and clinical information necessary for accurate diagnosis.
• 🚫 Mismatched information on labels or requisition forms can lead to sample rejection, emphasizing the need for accuracy.
• ❄️ Maintaining proper transport temperature for samples is critical, as improper temperatures can affect sample integrity.
• ⏱️ Timely transportation of samples within specified time frames is necessary to ensure sample viability and accuracy of results.
• 🔬 The quantity and quality of samples are important; insufficient or contaminated samples may be rejected and require recollection.
• 🩸 Specific techniques are required for collecting different types of samples, such as abscess, blood, body fluids, and urine, each with unique considerations for sterility and transport.

Q & A

What are universal precautions that should be taken while collecting samples?

Universal precautions include using barrier protection, wearing gloves when handling body fluids, wearing a face mask or goggles, and being cautious when handling sharp objects like needles and syringes.

Why is it important to clean the work area and use disinfectants?

Cleaning the work area and using disinfectants is crucial to prevent cross-contamination and ensure the accuracy of the samples collected.

What should be included in proper sample labeling?

Proper sample labeling should include the patient's name, clinical specimen type, the site where the sample was taken, a unique ID number, specimen type, date, time, and place of collection, and the initials of the person collecting the sample.

Why is the requisition form important for sample processing?

The requisition form is important because it provides essential patient information and clinical history, which are necessary for accurate diagnosis and reporting.

What are the common types of body fluids that may require collection for microbiological analysis?

Common types of body fluids for collection include blood, urine, ascitic fluid, pleural fluid, and pericardial fluid.

How should blood samples be collected for culture?

Blood samples for culture should be collected using a sterile technique, with the appropriate volume depending on the patient's age and condition, and transported to the laboratory in specific culture bottles.

What are the different methods for urine sample collection and which is the most common?

The different methods for urine sample collection include clean-catch midstream, catheterization, and suprapubic aspiration. The most common method is clean-catch midstream urine.

Why is it important to avoid contamination when collecting stool samples?

Contamination of stool samples can lead to false-positive results, so it's important to collect the sample in a sterile container and avoid contact with disinfectants or the toilet environment.

What are the criteria for rejecting a sample in microbiology?

Samples may be rejected if there is a mismatch of information on the label and request form, improper transport temperature, delay in transportation, insufficient quantity, leakage, or if collected without a requisition form.

How should samples be transported to ensure their integrity?

Samples should be transported promptly to the laboratory using appropriate containers and transport media, and kept at the correct temperature as specified for the type of sample.

Outlines

00:00

🧪 Laboratory Sample Collection and Precautions

The speaker from Aaron or Mike Ability Department introduces the topic of sample collection, emphasizing the importance of universal precautions in healthcare settings, particularly in hospitals. Key points include the use of barrier protection, gloves when handling body fluids, and face masks. Caution is advised when handling sharp objects to prevent needle pricks. The speaker also highlights the need for proper cleaning of work areas and the importance of sample labeling with patient details, specimen type, collection site, and a unique ID number. The necessity of a requisition form for accurate diagnosis and reporting is also discussed, along with the importance of patient history and correct sample labeling to avoid mismatches and ensure proper sample handling and transportation.

05:02

🩺 Proper Sample Collection Techniques

This section delves into the specifics of collecting various types of samples, such as abscesses, pus, blood, and body fluids. The speaker explains the differences between aerobic and anaerobic samples and the importance of collecting samples from the correct sites. For abscesses, the necessity of cleaning the infected area with sterile saline or alcohol before collection is emphasized. When collecting pus, the use of syringes for aspiration is recommended. For blood samples, the speaker details the types of blood culture bottles available and the appropriate volumes of blood to be collected based on the patient's condition. The collection of body fluids like ascitic, synovial, pericardial, and pleural fluids is also discussed, with a focus on using aseptic techniques and proper transport within a specified time frame to ensure sample integrity.

10:02

🌡️ Urine and Stool Sample Collection

The speaker discusses the collection of urine and stool samples, highlighting the importance of clean catch midstream urine and the different methods for collecting urine, including catheterization and suprapubic aspiration. The need for proper patient instruction and aseptic technique is emphasized to avoid contamination. For stool samples, the speaker advises on the use of a clean, leak-proof container and the appropriate amount of sample to be collected. The importance of transporting samples promptly to prevent degradation is also covered, with specific instructions for handling liquid and formed stool samples.

15:03

🧫 Specialized Sample Collection: Stool, Gastric Aspirate, and Sputum

This part of the script focuses on the collection of specialized samples such as stool, gastric aspirate, and sputum. The speaker provides guidance on the appropriate containers and transport media for stool samples, with an emphasis on avoiding preservatives that can interfere with certain tests. For gastric aspirate samples, used primarily for TB detection, the speaker outlines the need for aseptic conditions and the use of a nasogastric tube. The collection of sputum samples is also discussed, with instructions on how to induce expectoration and the importance of avoiding saliva contamination. The speaker stresses the need for proper patient preparation and the use of hypertonic solutions to promote secretion for sputum induction.

20:04

🔬 Nasal, Pharyngeal, and Eye Sample Collections

The speaker addresses the collection of samples from the nasal, pharyngeal, and eye areas. For nasal samples, the use of polyester swabs and a gentle rotation technique is recommended. Pharyngeal samples require aseptic swabs and a specific suction technique. Eye samples should be collected under aseptic conditions and transported promptly to the laboratory. The speaker also covers the importance of proper patient positioning and the use of appropriate materials to prevent contamination and ensure accurate sample collection.

25:05

🏥 Final Remarks on Sample Collection and Transportation

In the concluding part, the speaker summarizes the importance of proper sample collection and transportation, emphasizing the need for aseptic techniques and adherence to guidelines to ensure sample integrity. The speaker invites any further questions and provides contact information for additional clarification, highlighting the importance of accurate laboratory testing in patient diagnosis and treatment.

Keywords

💡Universal Precautions

Universal precautions refer to standard procedures that healthcare workers follow to minimize the risk of exposure to bloodborne pathogens. In the video, these precautions are crucial for anyone collecting samples, emphasizing the use of barrier protection, gloves, and masks to prevent contact with potentially infectious materials. The script mentions covering wounds and using sterile materials as part of these precautions.

💡Sample Collection

Sample collection is the process of obtaining biological specimens from patients for laboratory analysis. The video script discusses various types of samples, including blood, urine, and swabs, and the importance of proper collection techniques to ensure the accuracy of diagnostic results. For instance, the script specifies the need for aseptic techniques when collecting blood samples to prevent contamination.

💡Transport Medium

A transport medium is a substance used to preserve and transport biological samples from the collection site to the laboratory without compromising the sample's integrity. The video mentions the use of transport media for various samples, such as urine and stool, to maintain the viability of microorganisms or cells for further analysis.

💡Labeling

Proper labeling of samples is essential for accurate identification and tracking within a healthcare facility. The script highlights the importance of labeling with patient details, sample type, collection site, and other relevant information to ensure that the correct analysis is performed and results are attributed to the right patient.

💡Requisition Form

A requisition form is a document that accompanies a patient sample to the laboratory, providing essential clinical information that guides the testing process. The video script stresses the necessity of a completed requisition form for every sample, including patient history and specific test requests, to enable the laboratory to provide an accurate and relevant report.

💡Sterile Technique

Sterile technique involves maintaining an environment free from microorganisms to prevent contamination during sample collection or medical procedures. The video script describes the use of sterile saline and alcohol for wiping the collection area and the importance of using sterile containers for sample storage, as part of maintaining a sterile technique.

💡Disinfectant

Disinfectants are chemicals used to kill microorganisms on surfaces to prevent the spread of infection. The video mentions the use of disinfectants to clean work areas and collection sites, such as applying disinfectant to the skin before inserting a needle for blood collection, to reduce the risk of introducing contaminants into the sample.

💡Specimen

A specimen is a sample of tissue, fluid, or other biological material taken from a patient for diagnostic testing. The video script covers various types of specimens, such as blood, urine, and swabs, and the specific requirements for their collection, transport, and handling to ensure the reliability of laboratory tests.

💡Microbiology Department

The microbiology department in a healthcare facility is responsible for analyzing biological samples to identify microorganisms and determine their role in disease. The video script discusses the importance of sending properly collected and labeled samples to the microbiology department for accurate diagnosis and reporting.

💡Contamination

Contamination refers to the presence of unwanted substances or microorganisms in a sample that can compromise the accuracy of laboratory tests. The video script warns against contamination by emphasizing the need for aseptic techniques, proper labeling, and the use of sterile equipment during sample collection and handling.

💡Aerobic and Anaerobic Cultures

Aerobic and anaerobic cultures are laboratory tests used to grow microorganisms under oxygen-rich (aerobic) or oxygen-free (anaerobic) conditions, respectively. The video script mentions the need for different types of culture bottles for these tests, depending on the microorganisms expected in the sample, such as abscesses that may require both aerobic and anaerobic cultures.

Highlights

Importance of universal precautions in sample collection and transportation.

Use of barrier protection and gloves to handle body fluids.

Necessity of face masks or goggles while collecting samples.

Caution while handling sharp objects like needles and syringes.

Proper disposal of condom native materials using biohazardous waste procedures.

Cleaning work areas with soap and water or disinfectants.

Thorough hand washing after handling samples.

Essential aspects of proper sample labeling for microbiology departments.

Requirements for patient name, clinical specimen, and unique ID number on labels.

Importance of requisition forms for accurate diagnosis and reporting.

Criteria for sample rejection due to mislabeling or improper transport.

Transport temperature requirements for different types of samples.

Avoiding delays in transportation and the impact on sample integrity.

Correct collection techniques for abscess samples, including swab and aspirate methods.

Instructions for collecting blood samples, including quantities and sterility requirements.

Different types of blood culture bottles and their appropriate uses.

Collection and transport guidelines for body fluids like ascitic and pleural fluid.

Urine sample collection methods, including clean catch, catheter, and suprapubic aspiration.

Proper handling and transport of stool samples for bacterial and parasitic analysis.

Collection techniques for sputum and gastric aspirate samples for TB detection.

Guidelines for collecting nasopharyngeal and oropharyngeal samples.

Contraindications and precautions for sample collection to avoid contamination and errors.

Final remarks on ensuring sample quality and accuracy in lab testing.

Transcripts

good afternoon everyone

what I am going to be discussed sample

collection and transportation

I am from Aaron or Mike ability

department tutor before going to be

topic we should know something about in

yourself precautions without any

universal precautions we don't go for

any other work mostly in hospitals okay

and what are universal precautions you

should take while collecting samples

first one is use barrier protection in

case of any work but once in working

area please cover that whopper notes by

using some other it reads like some

sterile materials next you should be

some gloves while handling body fluids

or some other condom metal materials

next you should be at some face mask or

gone without wearing any face masks are

gone you never take any sample and use

caution because while angling sharp

objects like needles and syringes or

some other thing okay you should take

some cautions because it should be taken

some needles needles pricking during

next discover condom native materials by

using by Assad miss are by or Sur

procedures next clean work area by using

some soap and water

if soap on what is not available then

use disinfectant then disinfectants not

available in Italy called respective

fellow to come and clean the respect to

areas then wash were and thoroughly its

soap and water after what gives you over

again each and after everything is over

then wash were close by using hot water

don't wash your clothes by using some

chilled water or ordinary water okay

before correcting sample labeling is

very important without proper labeling

you don't send any sample to micro micro

budget Department okay

then coming to labeling patient name is

very important we should write patient

improperly in bold letters don't write

any some giant letters then write

clinical specimen Part F of clinical

specimen whether it is in blood sample

whether it is an urine sample whether it

isn't per sample whatever we should

mention clearly then what site where you

an example whether you take sample from

left hand or right hand or lift lift

trick or like a right leg whatever you

shipment you should mention properly

then unique ID number in hospital each

and every patient a unique ID number is

there without any unique ID number you

don't send any sample to my committee

department then pessimal type you serve

what type of specimen then date time and

place of collection and name an initial

of character on what whether question is

ICU or OPD what operation Tector

whatever which should be mentioned

properly then we coming to requisition

form it is very important

without requisition form don't send any

sample to microbiology department based

on patient clinical esteem only we have

to diagnose we have to give accurate

report ok in that requisition form we

should fill all the information ok this

patient history is very important

without patient history don't send any

sample to Micro Department and signature

of consultant that is very important and

sign of the sample character who is

going to be correct sample and date and

time patient name patient ID age 6 word

sample or site and test request what

tests we want to share form ok these are

some criteria for inserting samples

which would remember all those things

while collecting samples too much and

sending to make up my geometry

department okay first you should know

mismatch of information on the label

under request this is very very

important because sometimes what

happened in that container should be

some other day our patient name and

request should be some other patient

name ok this should be avoided

next in a perfect transport temperature

sometimes in some blood blood sample is

in 4 degree Celsius

let them be using some ice bag in some

in some sample circuit 37 degree Celsius

you use by some other appropriate

technique in some sample using room

temperature is no problem you have to

same sample by room temperature then

access the delay in transportation

notice in all the sample it will 2 hours

if going about 2 hours they are not

responsible in appropriate transport

medium

suppose element dissident fixating agent

or pessimal should be in the right

condition our sample should be some

questionable questionable only we don't

know what type of sample that everything

should be rejected an insufficient

quantity in CSF okay no problem we have

to take 0.5 to 1 ml in your example you

don't send 0.5 to 1 ml at least we sent

5 ml of sample then leakage that is very

important in case of any leakage we

never accepting that sample then without

any requisition form see by using this

book transfer a specimen then we move to

one by one how to collect sample that

how to transport and all we are to

reserve first - abscess in abscess means

the tribulation own post fuels of ulcers

ok

the abscess should be two types aerobic

and anaerobic in arabic may be

superficial I never make me say it

should be depletions ok before

collecting sample we should wipe with

sterile saline alcohol the infected area

then go to take swaps rub along the edge

of the hole after taking sample we

should be transport within 2 hours and

we require 2 straps if we send one swabs

on recent of the three of us it should

be rejected in case of only one step ok

then you immediately call to my cabbages

you have to explain the condition of the

patient then when we accept other ways

me never accept any samples here see ya

based on fashionista money we give

reports in case of post-operative

patient conscious significant

without any patient history or we give

report they ought to give only no growth

next coming to pus

but mostly post should be aspirated by

using syringes only in case of closer

space abscess

what happened which would thicken

ammonite skin by using sterile saline or

alcohol then insert needle and aspirate

the first part of a sufficient amount

you take and send it to my compulsion

Department in case of open wound

remove superficial exudate okay aspect

through marginalist margin while you

sings sir ng or if there is no purse we

can take swaps and yo to rub it and send

it to my cabinet department see this is

an expected purse I'm coming to blood

sample in blood sample my cables you

mostly using for culture only and next

thing is serves a reactions me that

antigen antibody reactions I'm coming to

culture in our department some that

touch a bottle is available that is

especially suitable for culturing

bacteria bacteria swoon living okay in

the blood culture bottle we have three

types of bottles available in our

department one is arabic bottle another

big bottle and periodic bottle you come

and ask my culture department we want to

we want here a big bottle we want an

anaerobic bottle periodic but whatever

you want you come at nasse my comedy

department we will provide in case of in

fact you should take 0.5 to 2 ml in case

of children you take 2 to 5 ml in case

of forward you take 5 to 10 ml ok this

blood should be taken by wind puncher

only I'm coming to depression of in

puncher first you have to look at skin

you have to pail plate whether it's

serving appropriately then use alcohol

for 5 minutes then play for 30 to 60

seconds then take tension of IOD you

have to apply to scheme again right for

45 to 60 seconds then without poly

plating okay you have to take 20 ml of

blood and of this 20 ml 10 ml enacted

into arabic bottle aunt animal should be

knocked at end in a big bottle this is

based on a patient condition one day you

have to take in case of Petula's very

sick when you take 5 ml okay so question

listen LT then you take 20 ml then this

was our ng

in shop container label bottle and send

it to laboratory see these are metal

check bottles in case of travel febrile

episode you take two set of two sets of

blood work in case of battle

endocarditis you take three sets of

blood cultures bottles

okay don't take any three sets of blood

within 24 hours

then come into body fluids there are so

many body fields available okay of this

only few body flicks I mentioned here

okay on it somatic field ascetic fate by

signing off a pericardial fluid pleural

fluid and see yourself if you want to

take in your body fluids same thing

first we apply disinfectant or sterile

saline

then insert needle then you have to take

aspirate how much amount you want to

take we want 5 to 10 ml you take 5 to 10

ml if you want 10 to 20 ml you take 10

to 20 ml how much amount you want you

take then immediately transfer into

sterile screwcap container if this crew

captain is not available then you die to

inducted into periodic blood culture

bottle system now label it properly and

transport within 15 minutes we send

after 30 minutes we are not responsible

you know to sustain sample again back to

respective words see this is sterile

container may be some needle escalators

I'm coming to urine sample collection of

urine sample should be very important

because sometimes what happens some

cross combination will take place okay

urine collection must be three types

first one is clean catch midstream urine

Nixon catheter is green that means

suprapubic expression urine you see one

by one first one is clean catch

midstream urine okay it is very

important because most of a time using

clean catch midstream urine monthly okay

instruction to patient is very important

instruction to patient clean period the

area with soap and water then tell

inform the patient to pass initial urine

into toilet then collect midstream urine

into sterile cup suppose the cup the

holding cup pass on should be down or

you have to touch in select cup okay Oh

to inform immediately to respect to

nursing stuff tell them to take another

new cup okay before collection label is

important because you are incurring what

happens sometimes it should be spoiled

entire continent during passing urine so

you know to avoid all those things

here to the label all those things

properly before collecting any samples

okay if we found more than three or four

types of bacteria okay what happened the

same ultimately we put into the

contamination

it's kindly simply paid sample okay

suppose inhibited sample also again

three to four bacterias then we give

significant growth see this is an

pictorial representation of how to

collect urine sample see was clean ever

pee with a little soap and water then

was initially urine into toilet

then you collect middle portion of the

urine okay then cross it properly then

label it and send it to laboratory now

coming to catheterize via urine okay it

is very important this was mostly useful

for patient we having in under like some

unrelated disease condition or some

children you have to use

mischaracterized urine okay

first they inform to clean the brain

Oracle AERS open water then apply some

sprayed or some sterile saline to

decontaminate then insert catheter into

bladder then discard initial URI then

collect specimens towel cup okay this is

for normal catheter during incoming to

chronic in Velenje catheter work in that

what happened you can take sample from

junction between kettle and collection

to you don't take any sample from this

discarding back okay if you come collect

urine from discoloring back mostly from

contamination so only because it is

24-hour collection what happened during

24 hours collection

mostly fuel and fuel contamination

smoothly so you don't track any sample

from this discarding back it should

collect sample from this junction

modeling then coming to suprapubic

aspiration sample this is mostly from

some unrelated business condition only

mostly in periodic patient okay those

who are unable to pass urine

okay what happened you have to follow

the full aseptic condition because they

are to insert needle into super pubic

region okay it should be little risk in

from the patient bladder should be

filled before performing this procedure

then papers came with alcohol or iodine

then give some little key for local

system then insert needle to drop and

two ml e 0.2 centimeters of the above

the synthesis then a spec 20 ml of urine

for culture then send it to laboratory

see this is a procedure how to take

urine by using super epic aspirations

then coming to scroll sample stool is

generally non sterile sample okay before

cutting stool sample we should know

something about stool okay what is a

normal floor of elementary cannot they

mainly we give reports without knowing

elementary can all normal flora we never

give any reports okay freshly possible

sample is very important avoid basement

from Big Bang bed pan is not a suitable

placement for culturing stool sample use

sterile or clean container okay

don't use any container with

disinfectant during outbreak you should

correct

10 to 20 percent stool samples I'm

coming to collection we take clean

leak-proof container in case of liquid

liquid you take one tablespoon that is 5

ml of stool sample in case of Foreman

stool you take pea-sized rule is

sufficient then after collecting the

transport within 30 minutes

if delay transfer fecal sample into

transport medium

don't send any sample for or don't send

any sample with preservative or

something say tubes if we send any

samples in preservatives or fixatives it

is not suitable for right Widman

examination president difficile toxin

reduction reduction of viruses elias on

lattice occupation tasters and coming to

bacterial culture mostly we encounter

Salmonella Shigella and Campylobacter if

doctor asking if specify specifying we

want be Brio we want aroma last report

we want to BZ amana's el Senor equal

o157 hs7

then that time only ought to process

otherwise we never possess this thing

suppose if doctor asking she got ox and

I say then you follow otherwise you

don't follow any Shiga toxin I see ok

this everything

should be recommended by CDC Center for

Disease Control suppose some people

giving report equally in that thing

and can't you go and confirm in doctors

what is a patient condition then we will

put as an equalising significant and

don't go don't give any grand

possibility basically a gram positive

cocci and coming to school for parasites

okay now would it take three perceptual

sample to one product we celebrate you

mostly using passivity here ten person

is firmly or Paul evening chloride okay

this unprecedented action and PCR this

present sample is not suitable for PCR

piece here and antigen detection and can

come in two vectors where this is mostly

suitable for small children and nibbled

at a patient and other situation

suggests those who unable to take it

from the bid that time you have to take

this rectal swab come into collection in

cells where approximately two point five

centimeter IELTS printer and figures

should be visible on the sweats they

know to take out immediately and

transport immediately without any delay

delay use the transport medium it is not

suitable for Paris le Direction talks in

toxic direction and my agents it should

be for only for culture smoothly Bechdel

culture only and coming to draw back

there is no microscopy assessment

possible unless material available on

out a chimera for viruses and coming to

gastric aspirate the gastric content

mostly useful for TB detection mostly

what happened some in children have

difficulty to expediting put them simple

so that time what happened we were to

recommend gastric has spread because

what happen in gastric the spread and

children with pneumonia sleep what

happened during Miko's Larry mechanism

okay the entire milk is sample what

happen comes into a participatory track

when reaches into throat region again

the swallowing the children then what

happened is a intestine to stomach

region okay that is enforced during

stomach region what happy contain which

source of TB TB organisms and come into

collection before collection instruction

is very important

in section 2 a patient or come early

morning an infirmity patient you should

sleep at least six hours before

collection okay and infirmity patients

should not eat or drink anything

overnight to prevent the stomach from

empty and come collection by using an

ass or gastric true okay

don't use any sub W because it is in

vector static it will kill all bacterias

and mostly rig you report going for

negative only use large bore nasogastric

tube don't do small sized strips okay

and white too deep because what happen

if we entered too deep and this on the

fire is raging okay what happen it will

take whatever condense into fire season

and you have to take from outside so it

leaving some false positive or false

negative a put during processing should

be too simple swim required for

processing one non each day suppose if

it is not possible in form to respect to

my cable this I mean from my shiny

condition is very difficult so it is not

possible to take two sample one is

sufficient of after taking sample you

have to transfer 5 to 10 ml gastric

fluid from syringe into sterile

container then add equal volume of

sodium bichromate solution because in

what happened acidic we ought to take in

sample from gastric content in gastric

content what happening because

increasing in pH increasing acidic pH so

neutralizes acidic pH by adding sodium

bicarbonate solution to prevent be the

section of table food basically

I am coming to spot em sample protein

sample is mostly taken by using two

methods one is induces put'em next one

is export a test bottom and coming to

export at this bottom it is very very

important most of the time a of you take

on coming to elaborate it is put on only

and this is a very risky also because

most of the time patient will come and

meet with saliva also so it requires

some initial technique and what happened

some good knowledge about patient and

nurses then we only be getting this

Goods put them and come into collection

we before collection it should be

informed to patient don't take any food

one two tours before taking any sample

ok mode should be rinsing with soap

saline or water and should instead to

patient to provide beef cuff ok and

weapon the cuttin L and keep it near to

the mote okay what happened doing diff

cover depends put him back Lee

come on contact with wide monster

container then immediately close that

tube and market market market properly

and send it to a laboratory many may

avoid avoid cyber contamination

suppose he have any saliva condemnation

it's it's not suitable for my ecology

laboratory we send it back to respect to

love what's see these are common

procedure cleaned my mouth and breathe

in and out three times then take a

sample then come into a certain area it

is a rejection criteria first put them

sample suppose if sample is sending to

microbiology laboratory s and remorse

saliva e and microscopy out to find out

more than ten squamous epithelial cell

it is rejected okay

come into excellent sample it should be

more than twenty five polymorphonuclear

cells plus few epithelial cells that is

five to six cells it is it is excellent

suppose if you find out more than ten

squamous epithelial cell on one or two

per cells it is not suitable for culture

it may be some saliva contaminations

okay and come in to induce report on

okay it is mostly suitable for patient

unable to produce bottom sample because

mostly it's the patient only it is

difficult to do in this put'em okay but

in that time are using some some partial

technique to take sport and sample okay

before collection patient should be

brushed the buccal mucosa

tank and gums and queen you the patient

some nibblies hypotonic solution what

happened this solution it will give for

the area secretion and promote the

coughing and allowing butum spittle

secretion to outside

I am coming to contraindications and

precautions while collecting samples

because induces put em be giving

hypertonic solutions only what happened

15 giving iPod on excellent solution

it causes Branco construction did this

manga construction what happened

perchance is going to be some critical

stage okay

before giving this iPod on external

solution in case of asthma patient or

some suspect asthma patient a civil lung

impairment function patient able to use

a medical premedication then mainly we

give you this Austral solution otherwise

I never give any sterile solution then

apart from this in case of severe

cufflink pressure don't form this

procedure okay

an apart from this patient young Emma

Topsy's accurate expedited stress

unstable cardiovascular acts and

abdominal a cerebellar infections and I

fucsia

run functional impairment pneumothorax

pulmonary emboli these are conditions we

never use this technique we go for

experts put and technique only I'm

coming to it is equation endotracheal

take secretion okay it mostly by you

that custom a patient mentally what

happened by using glue can trap you can

take sample okay what happened mostly

Tyco so my patient

mostly GN are present in this our mouth

region and apart from this this stack of

straw me equipments okay

it is not clinically revelant okay

sometimes what happen it will enters

into your lungs and cause pneumonia

during aspiration okay we have to

confirm the patient history and based on

the semi quality report we have to

correlate and new clinic miss on a semi

quality culture report on clinical

condition of the patient then we only be

out doing new clinic it is significant

other way see never give it is not

significant and coming to NASA

pharyngeal aspirate

okay this is also another is Polly

secretions what happened attach Amica

strapped into suction out like pitously

this is some suction tip okay we have to

attach Inlet and outlet okay in outlet

what happen inside into NASA pharyngeal

region in outlet which would connect

into some suction pressure okay with the

help of general section what happened it

will take all week a sample from a

prospective tract into this we can strap

this tube then after taking this thing

you have to cut this tube and

immediately transferred into laboratory

you have to transport within 15 minutes

if taken more than Tatem 30 minutes what

happened it should be condemnation only

so that time you have to reject sample

so you should take care while

transporting sample also now to place

the sampling ice box or in refrigerator

it's transportation delay and coming to

NASA fragile aspirate now NSF orange

else perhaps okay

infirm to a patient sit comfortably in

chair or some other our stool then tilt

helped backwards and insert flexible

finds shaft Polly stress wraps don't use

any cotton swabs okay if you use cotton

swabs in case of anaerobic

we do some wrong reports use some

polyester swabs into nostrils and back

to the nasa pharynx leave it for few

seconds and withdraw slowly by using

gentle rotation and coming to ear sample

in air sample in case of in air sample

over to clean in a year with soap and

water before functioning haradrim

okay then after washing you have to

puncture a spread material okay then

in case of is no pus then you're to take

swabs and go to Gen gentle rotate and

send it to laboratory in case of outer

air rotor it takes trial cotton swabs it

should be most units saline awesome

transport media and how to wipe the

crust with sterile saline monthly then

you have to gently rotate the swept into

outer cannot then you take gently

outside then transported to laboratory

immediately I am coming to ice samples

mostly using conductive also apps it res

fluids on corner scraping only this we

require some full aseptic conditions

aren't fully precautions without using

any precaution don't take any eye

samples those who express that represent

only taking this samples okay after

taking this sample transport within 50

minutes to laboratory if you delay don't

send any sample to laboratory otherwise

you keep it in refrigerator then we same

sample after 30 minutes to one another

whatever but before whenever you send

sample to a laboratory if you have any

clarification okay see this website okay

in this episode of 114 videos regarding

about sample collection and lab

management system ok if you have further

any doubts come and ask me in Department

thank you