Parkinson's Research Model in Zebrafish
Summary
TLDRThis video delves into Parkinson's disease, exploring its progression and impact through a zebrafish animal model. It discusses the disease's genetic and environmental factors, dopamine deficiency, and the use of MPTP to induce phenotypic changes. The script covers behavioral tests to observe symptoms like tremors, muscular rigidity, and anxiety, highlighting the model's significance in Parkinson's research and potential for therapeutic development.
Takeaways
- 🧠 Parkinson's disease is the second most common neurodegenerative disorder, characterized by the degeneration of the substantia nigra and dopamine deficiency.
- 🔍 The etiology of Parkinson's is not fully understood but involves genetic, environmental, and aging factors, along with toxins.
- 🐟 The zebrafish animal model is used to study Parkinson's disease, with phenotypic changes induced by MPTP leading to dopamine neuron damage.
- 🧬 Genetic changes in the zebrafish model include alterations in the expression of alpha-synuclein isoforms, which may compensate for motor impairments.
- 💊 The model exhibits both motor and non-motor symptoms, such as tremors, muscular rigidity, and anxiety, which progress with the disease.
- 📊 Behavioral tests in the zebrafish model, like the novel tank test, can indicate anxiety and other stress responses associated with Parkinson's.
- 🔬 Post-mortem analysis of the fish brain can reveal the presence of degenerative cells and Lewy bodies, typical of Parkinson's pathology.
- 🚶♂️ Postural instability and gait disturbances are observed in the model, reflecting advanced Parkinson's symptoms in humans.
- 🔄 Stereotyped movements, repetitive behaviors, are indicative of the motor impairments seen in Parkinson's disease.
- 🤖 The zebrafish model is valuable for screening potential treatments and understanding the progression of Parkinson's disease.
- 🌟 Advancements in the zebrafish model with both phenotypic and genotypic changes offer promising avenues for translational research in Parkinson's.
Q & A
What is the main topic discussed in the video script?
-The main topic discussed is Parkinson's disease, particularly focusing on phenotypic changes in a zebrafish animal model of the disease.
What is Parkinson's disease?
-Parkinson's disease is a slowly progressive neurodegenerative disease associated with the degeneration of the basal ganglia of the brain and a deficiency of the neurotransmitter dopamine. It is the second most common neurodegenerative disease after Alzheimer's.
What are some factors that contribute to the etiology of Parkinson's disease?
-Factors contributing to the etiology of Parkinson's disease include genetic mutations, environmental triggers, aging, and toxins.
What animal model is used in the study of Parkinson's disease in this script?
-The animal model used is the zebrafish.
What compound is used to induce Parkinson's-like symptoms in the zebrafish model?
-The compound used to induce Parkinson's-like symptoms in zebrafish is MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine).
What are some phenotypic changes observed in zebrafish with Parkinson's disease?
-Phenotypic changes observed include abnormalities in body posture, tremor, muscular rigidity, movement loss, and anxiety.
How is tremor assessed in the zebrafish model?
-Tremor is assessed by introducing the zebrafish into a small tank and observing the number of tremors, particularly in the tail region, in response to a given time.
What method is used to evaluate muscular rigidity in zebrafish?
-Muscular rigidity is evaluated by introducing the fish into an experimental tank and counting the number of times the fish bends in response to a touch stimulus using a blunt capillary tube.
What behavioral assay is used to assess anxiety in zebrafish with Parkinson's disease?
-The novel tank diving test is used to assess anxiety. This test measures the time spent by the fish in the dark zone versus exploring the tank, with more time in the dark zone indicating anxiety.
What changes are observed in the brain histology of zebrafish with Parkinson's disease?
-The brain histology of zebrafish with Parkinson's disease shows aggregates of degenerative cells and abnormal cell development compared to normal cells in the control fish.
What is postural instability, and how is it observed in the zebrafish model?
-Postural instability is the loss of postural reflexes, leading to balance issues. In the zebrafish model, it is observed by recording the fish's body stability during both static and dynamic tasks.
What is the significance of stereotyped movement in Parkinson's disease zebrafish model?
-Stereotyped movement, which is repetitive and purposeless, is a significant symptom indicating behavioral changes in the zebrafish model of Parkinson's disease.
What is bradykinesia, and how is it studied in the zebrafish model?
-Bradykinesia refers to the slowness of movement, a hallmark of Parkinson's disease. It is studied by counting the number of quadrant changes the zebrafish makes in a divided tank within a given period.
Outlines
🧬 Parkinson's Disease and Zebrafish Animal Model
This paragraph introduces the topic of Parkinson's disease, a progressive neurodegenerative disorder characterized by the degeneration of the substantia nigra and dopamine deficiency. The zebrafish model is highlighted as a significant tool in studying the disease due to its phenotypic and genotypic changes post-MPTP induction, which mimic the human condition. The paragraph discusses the role of alpha-synuclein and the impact on the dopaminergic system, leading to motor impairments in zebrafish. It also outlines various behavioral tests used to assess Parkinson's disease symptoms in the model, including resting tremors, muscular rigidity, and uncoordinated movement, which are indicative of the disease's progression.
🔍 Behavioral and Neurological Assessments in Parkinson's Disease Models
The second paragraph delves into specific behavioral and neurological assessments used to evaluate the effects of Parkinson's disease in zebrafish models. It describes tests for anxiety, motor coordination, and postural stability, which are crucial for understanding the disease's impact. The paragraph explains how these tests, including the novel tank test and the assessment of stereotyped movements, help in identifying anxiety-like behavior and motor impairments associated with Parkinson's. It also touches on the use of brain cytology to observe degenerative cell changes and the importance of these models in advancing translational research for Parkinson's disease.
Mindmap
Keywords
💡Parkinson's disease
💡phenotypic changes
💡zebrafish animal model
💡dopaminergic neurons
💡MPTP
💡motor symptoms
💡alpha-synuclein
💡genetic mutation
💡resting tremor
💡anxiety
Highlights
Introduction to Parkinson's disease and its significance as the second most common neurodegenerative disease.
Parkinson's disease is associated with the degeneration of the basal ganglia of the brain and deficiency of the neurotransmitter dopamine.
Etiology of Parkinson's disease remains unknown, but factors include genetic mutation, environmental triggers, aging, and toxins.
Use of zebrafish as an animal model to study phenotypic changes related to Parkinson's disease.
MPTP used to induce Parkinson's disease in zebrafish, leading to the generation of reactive oxygen species.
Destruction of dopaminergic cells is a key aspect of Parkinson's disease progression.
Humans with Parkinson's disease exhibit motor and non-motor symptoms.
Zebrafish model exhibits both phenotypic and genotypic changes post-induction.
Alpha-synuclein plays a significant role in genetic changes observed in Parkinson's models.
Zebrafish alpha-synuclein is closest to human alpha-synuclein in function.
Motor impairments in zebrafish become more severe with the expression of alpha-synuclein.
Phenotypic changes in zebrafish include abnormalities in body posture, tremor, muscular rigidity, movement loss, and anxiety.
Experimental setups are used to validate the prognosis of Parkinson's disease in response to behavioral changes in zebrafish.
Resting tremor and action tremor are two types of tremors associated with Parkinson's disease.
Behavioral assays in zebrafish include response to hectic stimuli, jaw movement recording, and novel tank diving tests.
Transcripts
hi everyone this is your metal duck from
Metacritic I'm very happy to see you
back again now with our new topic in
position the topic for this issue is
based on Parkinson's disease up next our
position the topic for this session is
with a Parkinson's exhibit phenotypic
changes in zebrafish animal model
Parkinson's disease is a slowly
progressive second most common
neurodegenerative disease after as cheap
as it is associated with the
regeneration of the Bassel Campea of the
brain and the deficiency of the
neurotransmitter dopamine as a consensus
acrimony can progress if this is the
degree of disability varies among the
effector projects it is also known as
paralyzes residents unchanging path see
the etiology of Parkinson's remains
unknown but several factors play a role
including genetic mutation environmental
triggers aging and toxins of phenotype
changes during Parkinson's zip lavash
were in used with the MPTP after
induction the mood of our main oxidase
be thus taken up by other dopamine
transporter into dopamine neurons
whether it leads to the increased
generation of reactive oxygen species
mostly young BTK effects enter
dopaminergic system the progression of
Parkinson's disease occurs due to the
destruction of dopaminergic cells next
is modern development in Parkinson's
disease
humans with Parkinson's disease inhibit
motor symptoms as well as non-motor
symptoms signal fish animal model with
Parkinson's disease processes
motor symptoms comparatively progress
depends of human symptoms of post
induction the model starts exhibits both
phenotype and genotype changes any
abilities or changes in the function of
XX EEG that is simply alpha causes
genetic changes in Parkinson's model
signals which express threesome you
clean isoforms of beta gamma 1 and gamma
2 significance to compensate the axons
of alpha-synuclein functionally the
zebrafish alpha once a new clean appears
to be closest to the human signaling not
Amna of the beta I am gamma 1 see
nucleus in users motor impairments in
zebrafish
which are even more severe when the
expression of Watson you cleaners after
they have the tracking works and
nutrients have an abnormal development
of the dopaminergic system including
delayed differentiation of dopaminergic
neurons and reduced levels of the common
which results in Parkinson's disease
phenotype exchangers include the
abnormalities in the body posture tremor
muscular rigidity movement loss and
anxiety which are observed with the
progression of the disease model here I
am going to take you through the
phenotype it changes in zebrafish animal
border with packing since this is
different experimental setups are used
to validate the prognosis of Parkinson's
disease in response to the behavioral
changes in the model some of that this
represents the prognosis of Parkinson's
disease with respect to the phenotype of
the asli's are interesting Jemma number
of severe in response to a tech
stimulate a muscular rigidity number of
for job access or uncoordinated movement
died contest psychology of the brain
host of instability stereotype movement
and radical miss yeah first is trauma
ademir is not involuntary quivering
movement or shape or uncontrolled muscle
contraction which manifest as shaking in
body parts there are two types of
tremors occurs in Parkinson's disease
resting tremor and action/drama
the resting tremor is more commonly
associated with Parkinson's disease but
there are action tremor is less common
in some cases there is a probability of
internet drama which is not noticeable
to others in this as we observe the
Equality Movement in the zebrafish the
model is introduced into the small
oxalate attack and observed for number
of temes at a given time the trauma
mostly occurs in the tail region in a
cadenza crayfish on the screen is the
graph between control and water in
response to trauma we observe resting
tremor occurs in the model with
Parkinson's disease next is number of
ribbon in response to hectic stimuli or
muscular rigidity
cement and not which the fish body
follows the curves to form a thin shape
and then the fish propels itself a bit
at an angle from its previous position
using your fastest book using the text
stimulus the fish pond cement in order
to exhibit escape response the fish is
introduced into the small experimental
tank from the housing
and the head was gently touch using no
bland capillary tube the number of see
been in response to a touchback counter
on the screen is the graph between
control and water hundred show sleepin
in response over each touch that the
model shows this response of wind
compared to control this indicated the
development of muscular rigidity which
one of the primary symptoms in
Parkinson's
next is behavioral passes on Chama
mentor Parkinson's disease often leads
to involuntary under the pincer
movements of uncoordinated movement may
be the sign of the starter motor
response that causes an inability to
coordinate vector the study which was
introduced into us more on experimental
time and the jaw movements were recorded
for a period of 10 minutes the number of
job causes was countered from the video
recorded on the screen is the graph
between control and model the motor
shows more behavioral processes on John
come back to control indicating rigidity
of fine verses and in warranty passes
next is nor by die contest the novel
type time test extracts the stress
response that is exactly known you know
regenerative response in normal die tank
the tank is filled with water and
separated into two zones that is drops
of high water surface the night spent in
the upper portion of the gun latency to
reach the upper portion of the tank
number of transitions into the upper
portion and number of erratic humans for
motor using them all by that cactus
the fish is introduced into the study
tank from the housing pack and calculate
the time spent by the fish in the water
and dogs one of the tank up on the
screen is the graph between control and
model the control spends little time in
the dark zone and starts to explore the
tank as a natural behavior whereas the
murder
in contrast prefer to spend more time on
the dark zone of the time indicating the
presence of anxiety makes the psychology
of the brain the elevated level of
factors in the brain may be an early
sign of Parkinson's disease the brain
cytologists now started out in response
to find the nature of cells in water the
fish was euthanized at 4 degrees celsius
and the brain was dissected and fixed on
plastics
net oxidant and yoson stains values -
for differential staining on the screen
is the path
of the brain factor 8 and modern
representing the Uranus's under shows
normal cells where the water shoots
aggregates of degenerative cells and
also the presence of an in-between
develop fat cells
next is postural instability boostin
instability is one of the cardinal
manifestations of Parkinson's disease
you do the laws of hoster reflexes
although it is not common in the early
stages it becomes a clinical concern in
the middle later stages of Parkinson's
disease models with the Parkinson's
disease Humpty please do stability
during both static and dynamic tasks
this assay is performed to observe the
presence or absence of question
instability the fissures introduced into
the experiment attack from the housing
using the neck the unstable portion of
the fish is recorded in a video on the
screen is the video which is represents
the instability in the body portion of
the model and graph represents the
presence of posters and stability model
and access control next stereotyping by
data
stereotyped movement is the repetitive
purposeless movement it is one of the
oppressive or repeated movement symptoms
in Arkansas border occurs in at one
stage the fissures introduced into the
experimental dance from the housing from
the number of stereotyped movement
occurs in a fish for a minute after the
adaptation period of one minute of the
time on the screen is the graph shows
number of stereotyped movement between
hunter and model model shows to detect a
pattern of behavior which is one of the
phenotype biomarkers
next is very famous love daddy Venezia
is known as the movement and as one of
the cardinal manifestations of
Parkinson's diseases it is associated
with that impaired ability to access the
body's position to study the data pain
is not the experimental fishtank was
divided into four quadrants with solid
lines and calculate the number of
quadrant changes for a given period of
time the study fish was introduced into
the experimental tank from the housing
can you see the delta home the number of
quadrants changed by the fish for three
minutes so on the screen is the graph
between control and model representing
the average quadrant change is decreased
in the model in comparison to the
control this indicates of restricted
movement of the modern in comparison to
Parkinson's disease represents the most
frequent hypokinetic syndromes
these include Hakeem Islam gladion is
not gait and balance disturbances
phasing phenomenon and acidity it is
concluded that the market of Parkinson's
phenotypes or observing a zebrafish
animal oil but Parkinson's discusses the
signal which model with phenotype
changes and observed in Parkinson's is
variegated and is widely used in
screening we paint the target index of
translational research is quite
promising with advancement in the model
with both phenotype angelo exchanges
thank you this is metal designing from
you and see you back again with next
talk
[Music]
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