Praktikum Pemeriksaan hematokrit

Summary

The video tutorial covers the practical aspects of hematocrit testing, highlighting both macro and micro methods. It details the equipment needed, including a centrifuge and specialized hematocrit tubes. The process begins with blood sample preparation, emphasizing proper handling and filling techniques to ensure accuracy. After centrifugation, viewers learn how to read the results accurately by aligning the plasma and cell boundaries on the hematocrit reader. The session concludes with a demonstration of calculating hematocrit percentages, showcasing the significance of the test in clinical practice.

Takeaways

• 🔬 The practical session focuses on hematocrit examination, which can be performed using two methods: macro and microhematocrit.
• 🧪 The equipment needed for microhematocrit includes a centrifuge and hematocrit tubes with specific markings.
• 💉 Before drawing blood, it is essential to homogenize the sample by inverting it 5-8 times.
• 📏 Blood is drawn into the hematocrit tube up to two-thirds of its capacity.
• 🧼 The end of the hematocrit tube should be cleaned with tissue to avoid contamination before proceeding.
• 🔒 It is important to seal the end of the hematocrit tube securely before centrifugation to prevent sample loss.
• ⚖️ A balancing tube must be used opposite the hematocrit tube in the centrifuge to maintain equilibrium during spinning.
• ⏱️ The centrifuge should be set to 1600 RPM and run for 3-5 minutes for accurate results.
• 📊 After centrifugation, the hematocrit level is read from the hematocrit reader, ensuring proper alignment at the zero line.
• 📈 The final hematocrit value is reported as a percentage, with an example given of 47%.

Q & A

What is the purpose of the hematocrit test?

The hematocrit test measures the proportion of blood volume that is occupied by red blood cells, providing important information about a person's health.

What are the two methods of hematocrit testing mentioned?

The two methods are macrohematocrit and microhematocrit.

What equipment is used for the microhematocrit method?

The equipment includes a microhematocrit centrifuge, hematocrit tubes, and a hematocrit reader.

How should blood be prepared before using it for the hematocrit test?

The blood sample should be homogenized by gently inverting it 5-8 times before it is drawn into the hematocrit tube.

What is the significance of filling the hematocrit tube to two-thirds full?

Filling the tube to two-thirds full ensures that there is enough sample for accurate measurement during the centrifugation process.

What precautions should be taken when placing the hematocrit tube in the centrifuge?

The hematocrit tubes should be placed with the open end facing outward, and a balance tube should be used on the opposite side to maintain equilibrium.

What is the recommended centrifuge speed and duration for this procedure?

The recommended centrifuge speed is 1600 RPM for a duration of 3-5 minutes.

How is the hematocrit value read after centrifugation?

The hematocrit value is read by aligning the boundary between the red blood cells and plasma with the zero line on the hematocrit reader, then reading the corresponding value on the scale.

What does a hematocrit value of 47% indicate?

A hematocrit value of 47% indicates that 47% of the blood volume is made up of red blood cells, which is within the normal range for many adults.

What should be done if there is a mistake during the hematocrit testing process?

If a mistake occurs, it is important to document it and ensure that the sample is prepared and tested correctly in subsequent attempts.