Difference Between Density Gradient Centrifugation and Differential Centrifugation

Summary

This video explains the differences between density gradient centrifugation and differential centrifugation. Density gradient centrifugation separates particles based on density using a sugar solution, while differential centrifugation separates particles based on size without a sugar solution. The former is more difficult and used for molecules or large particles, whereas the latter is easier and used for cells, organelles, and macromolecules.

Takeaways

• 📊 Density gradient centrifugation separates particles based on their density.
• ⚖️ Differential centrifugation separates particles based on their size.
• 🧪 Density gradient centrifugation was invented by Miron Brachet in 1951.
• 🕰️ Differential centrifugation was introduced by Bensley in 1934.
• 🌡️ The separation technique for density gradient centrifugation relies on the density of particles.
• 📏 The separation technique for differential centrifugation relies on the size of particles.
• 🍬 Sucrose or other sugar solutions are used in density gradient centrifugation, but not in differential centrifugation.
• 🔄 Two types of density gradient centrifugation exist: rate zonal centrifugation and isopycnic centrifugation.
• 🧩 Density gradient centrifugation is used to separate large particles and molecules, while differential centrifugation is used to separate cells, organelles, and macromolecules.
• 🧫 Applications: Density gradient centrifugation is for homogeneous solutions, while differential centrifugation is for homogenized organs.

Q & A

What is density gradient centrifugation?

Density gradient centrifugation is an analytical technique where particles are separated in a medium depending on the density of the particle.

Who invented density gradient centrifugation?

Miron Bracha invented the first kind of density gradient centrifugation in 1951.

What is differential centrifugation?

Differential centrifugation is an analytical technique where particles are separated in a mixture depending on the size of the particle.

Who introduced differential centrifugation?

Differential centrifugation was first introduced by Bensley in 1934.

What is the basis of separation in density gradient centrifugation?

The separation in density gradient centrifugation is based on the density of the analyte particle.

What is the basis of separation in differential centrifugation?

The separation in differential centrifugation is based on the size of the analyte particle.

Is there a sugar solution used in density gradient centrifugation?

Yes, a sugar solution like sucrose is used in density gradient centrifugation.

Is there a sugar solution used in differential centrifugation?

No, there is no sugar solution used in differential centrifugation.

What are the two types of density gradient centrifugation mentioned in the script?

The two types of density gradient centrifugation are rate zonal centrifugation and isopycnic centrifugation.

Is there a difficulty level associated with density gradient centrifugation?

Yes, density gradient centrifugation is considered difficult to use.

Is there a difficulty level associated with differential centrifugation?

No, differential centrifugation is considered easy to use.

What are the applications of density gradient centrifugation?

Density gradient centrifugation is used to separate molecules or large particles and is used for homogeneous solutions.

What are the applications of differential centrifugation?

Differential centrifugation is used to separate cells, organelles, and macromolecules and is used for homogenized organs.