The effect of Temperature on Invertase enzyme

SCC Science Vacaville
29 Apr 202108:26

Summary

TLDRThis video explores how temperature affects the activity of the enzyme invertase in a sucrose solution. The experiment involves using various temperature settings, from refrigeration to boiling, to observe enzyme performance. Key materials include a spectrophotometer, test tubes, and DNS reagent. After incubating samples at different temperatures and measuring absorbance, results indicate that 50 degrees Celsius is the optimal temperature for invertase activity. The video provides a detailed step-by-step guide to conducting the experiment safely and effectively, making it a valuable resource for understanding enzyme kinetics.

Takeaways

  • đŸŒĄïž Temperature significantly impacts the activity of the enzyme invertase on sucrose solutions.
  • 🍬 Invertase is derived from yeast and plays a role in candy production.
  • 🔬 Essential equipment includes a spectrophotometer, test tubes, cuvettes, and safety gear like gloves and goggles.
  • 🧊 Solutions are incubated at various temperatures: 4°C, room temperature (20°C), 50°C, and 100°C.
  • ⏳ Each temperature group is incubated for 15 minutes before adding DNS (dinitro salicylic acid).
  • đŸ”„ DNS reacts with glucose, and its reduction can be measured using a spectrophotometer.
  • đŸ§Ș Proper labeling of test tubes and cuvettes is crucial to avoid confusion during the experiment.
  • 🔄 The spectrophotometer should be zeroed using a blank solution containing only sucrose and buffer.
  • 📈 The optimal temperature for invertase activity is identified as 50°C based on absorbance readings.
  • ⚠ Safety precautions are necessary when handling corrosive substances and high-temperature equipment.

Q & A

  • What is the purpose of the experiment described in the video?

    -The experiment aims to investigate how temperature affects the activity of the enzyme invertase on a sucrose solution.

  • What source is invertase obtained from?

    -Invertase is obtained from yeast.

  • What safety equipment is recommended for this experiment?

    -Recommended safety equipment includes gloves, goggles, lab coats, and the use of a fume hood.

  • What equipment is needed to conduct the experiment?

    -Essential equipment includes a spectrophotometer, water baths, test tubes, glass cuvettes, and pipettes.

  • What is the first step in preparing the test tubes for the experiment?

    -The first step is to label the test tubes according to the temperature groups being tested.

  • What solutions are added to the test tubes during the experiment?

    -1 ml of sucrose solution, followed by a pH 5 buffer (1 ml for test tube 1 and 0.5 ml for test tube 2), and 0.5 ml of invertase suspension only to test tube 2.

  • At what temperatures are the test tubes incubated during the experiment?

    -Test tubes are incubated at 4°C, 20°C, 50°C, and 100°C.

  • Why is dinitro salicylic acid (DNS) added to the test tubes?

    -DNS is added to reduce glucose, and its reduced form is measurable and visible on a spectrophotometer.

  • What is the optimal temperature for invertase enzyme activity according to the results?

    -The optimal temperature for invertase enzyme activity is 50°C.

  • How is the spectrophotometer prepared for measuring absorbance?

    -The spectrophotometer is zeroed using a blank cuvette containing only sucrose and pH buffer before measuring the absorbance of the other samples.

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Étiquettes Connexes
Invertase ExperimentEnzyme ActivityTemperature EffectsSucrose SolutionLaboratory ProcedureBiochemistry StudyScientific ResearchEducational ContentSafety PracticesSpectrophotometry
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