Hitung Jumlah Leukosit - TUTORIAL LENGKAP PLUS PERHITUNGAN & TAMPILAN SEL LEUKOSIT LANGSUNG

Summary

In this educational video, Abdi from Universitas Muhammadiyah Semarang leads a practical session on counting white blood cells (leukocytes) using a hemocytometer. The process involves preparing blood samples, diluting with reagents, and using a microscope to count the cells. The video covers step-by-step procedures, including proper use of tools, how to count leukocytes in the hemocytometer, and the calculation of the cell count using a specific formula. The session also emphasizes the importance of clean equipment and accurate sample preparation, concluding with a brief guide on preparing practical reports.

Takeaways

• 😀 The main objective of the practical session is to count the white blood cells (leukocytes) in a patient's blood sample.
• 😀 The tools required for the experiment include a hemocytometer, Thoma pipettes (with red and white tips for erythrocyte and leukocyte counting respectively), Petri dish, aspirator, and various reagents.
• 😀 Reagents should be checked to ensure they are not expired, and the diluent solution should be clear and not cloudy before use.
• 😀 Before starting, it’s essential to ensure all tools are clean and dry, including the hemocytometer, which should be gently wiped, not scrubbed.
• 😀 A proper blood sample should be non-lysed, with no clots, and the correct ratio of blood to anticoagulant should be used.
• 😀 The practical procedure involves preparing the hemocytometer, pipetting the blood and reagent, and performing a one-minute incubation.
• 😀 Once incubation is complete, a drop of the blood-reagent mixture is placed on the hemocytometer slide and incubated in a moist Petri dish for 5 minutes.
• 😀 The blood sample is then examined under a microscope, starting with low magnification to orient the view and adjust the focus.
• 😀 The leukocyte count is conducted by focusing on specific counting chambers of the hemocytometer, using both high and low magnification as necessary.
• 😀 The leukocyte count is determined by counting the cells in four corner squares of the counting grid, applying the dilution factor and calculation formula to find the total leukocyte count per cubic millimeter.
• 😀 The results should be compared to the normal leukocyte range for an adult male, which is typically between 4,000 and 10,000 cells per mm³. A value below this range indicates leukopenia (low leukocyte count).

Q & A

What is the main objective of the laboratory procedure described in the transcript?

The main objective is to count the number of white blood cells (leukocytes) in a blood sample using a hemocytometer and a microscope.

What are the key tools required for this leukocyte counting procedure?

The key tools include a hemositometer (Improved Neubauer chamber), Thoma pipettes (with white tips for leukocytes), a petri dish with tissue for incubation, an aspirator for pipetting, a counter for cell counting, reagents for dilution, and a microscope.

How should the blood sample be prepared before performing the leukocyte count?

The blood sample should be free from lysis and clots. The blood should be mixed with the appropriate amount of anticoagulant, ensuring a proper blood-to-anticoagulant ratio.

Why is it necessary to incubate the blood sample in a petri dish with moistened tissue?

The incubation helps the blood cells settle in the chamber and prevents the chamber from drying out by maintaining humidity, which is crucial for accurate counting.

What is the purpose of using the Thoma pipette with a white tip in this experiment?

The Thoma pipette with a white tip is specifically used for counting leukocytes, as it is designed for accurate measurement of blood samples mixed with dilution reagents.

How is the leukocyte count conducted under the microscope?

The leukocytes are counted within a specific grid area on the hemocytometer. The procedure involves counting the cells in the 4 central squares, with cells touching the left and top boundaries of the squares being included in the count.

What is the formula used to calculate the total leukocyte count?

The formula used is: Total Leukocyte Count = (N / V) × P, where N is the total leukocytes counted in the 4 squares, V is the volume of the chamber, and P is the dilution factor.

What is the normal range for leukocyte count in adults?

The normal range for leukocytes in adults (both males and females) is between 4,000 and 10,000 leukocytes per cubic millimeter (mm³).

What should be done if the leukocyte count is outside the normal range?

If the leukocyte count is below 4,000 or above 10,000 leukocytes per cubic millimeter, further clinical interpretation and diagnostic tests are necessary to determine the underlying condition.

How should the laboratory equipment be cleaned after completing the leukocyte counting procedure?

The equipment, such as the hemocytometer and pipettes, should be cleaned with mild soap and water. The hemocytometer should be rinsed and dried with tissue paper, and the pipettes should be cleaned with distilled water and air-dried.