Uji Resistensi Vektor Terhadap Insektisida Metode CDC Bottle Assay - Balai Litbangkes Banjarnegara

Summary

The transcript details a method for testing mosquito resistance to insecticides using CDC bottle bioassays. It outlines the steps for preparing and coating test bottles with insecticide, creating stock solutions, and conducting the tests with specific mosquito species. The procedure emphasizes proper handling and monitoring of mosquito mortality rates to assess resistance levels. Results categorize resistance as susceptible, suspected resistant, or resistant, guiding further insecticide management strategies. This method is vital for effective vector control in public health.

Takeaways

• 🧪 The CDC bottle test assesses mosquito resistance to insecticides by determining if mosquitoes are still susceptible or resistant.
• 💧 The testing process starts with cleaning the bottles to ensure no dead mosquitoes are found post-wash.
• 🔬 Proper drying of bottles is essential to meet testing standards, which may involve air drying or using an oven.
• 📏 Stock solutions of insecticides are prepared by diluting according to specific concentration formulas.
• 📝 Labeling is crucial: four bottles are used for treatment and one for control, each properly marked for identification.
• ⚗️ Bottles are coated with insecticide, ensuring even distribution and drying before testing.
• 🐜 Each test requires 10-25 female mosquitoes of specific age and conditions for accurate results.
• ⏱️ The test duration includes monitoring mosquito mortality at intervals of 15 minutes up to 2 hours.
• 📊 Results are interpreted based on mortality rates: 98% or more indicates susceptibility, while below 80% indicates resistance.
• 🔄 If resistance is suspected, further testing is recommended using increased dosages to assess resistance intensity.

Q & A

What is the purpose of the CDC bottle test mentioned in the transcript?

The purpose of the CDC bottle test is to evaluate the resistance of mosquito vectors to insecticides, determining whether the tested mosquitoes are resistant or still susceptible, and to assess the intensity of resistance.

What are the initial steps for preparing the CDC bottles for testing?

The initial steps include washing the bottles with warm water and a little soap, soaking them for about 2 hours, and then rinsing them with tap water three times.

How should the bottles be dried after washing?

The bottles can be dried at room temperature overnight or in an oven at 50°C for 15 to 20 minutes. They should be checked for cleanliness by introducing susceptible mosquitoes.

What is required to create a stock solution of the insecticide?

To create a stock solution, the insecticide must be diluted to achieve the desired concentration as specified in the diagnostic dose, using a suitable solvent such as acetone or absolute ethanol.

How many bottles are used in the experiment and how are they labeled?

A total of five bottles are used: four for the treatment and one for the control. The control bottle is labeled 'K', and the treatment bottles are numbered 1 to 4 along with the test date.

What does the coating process of the bottles involve?

The coating process involves applying the insecticide solution inside the bottles and ensuring it coats the surface evenly. Each treatment bottle receives one milliliter of the stock solution.

What conditions should be maintained for the coated bottles before testing?

Coated bottles should be stored in a dark place with the lids opened if not used immediately. They should be used within five days of coating.

What criteria determine whether a mosquito is considered dead during the test?

A mosquito is considered dead if it cannot stand or move when the bottle is rotated. Additionally, if mortality exceeds 10% in the control bottle within two hours, the test must be repeated.

How is the mortality rate calculated during the resistance test?

The mortality rate is calculated by subtracting the percentage of mortality in the control from the percentage in the treatment and then dividing by 100, adjusted for control mortality.

What classifications are given based on the mortality rate of the mosquitoes?

If mortality is 98% or more, mosquitoes are categorized as susceptible. Mortality between 80% and 98% indicates suspected resistance, while less than 80% is classified as resistant.