Pemeriksaan Feses Metode Tidak Langsung (Pengapungan) Untuk Deteksi Telur Cacing

Summary

This video demonstrates a laboratory procedure for testing fecal samples using an indirect method. The process involves using a saturated NaCl solution to float parasitic eggs in feces. The sample is mixed, filtered, and left to settle. After 45-60 minutes, a microscope slide is prepared for staining with a 1-2% solution to examine the sample for eggs. The careful preparation ensures clear observation under a microscope, providing an essential tool for identifying intestinal parasites in a non-invasive manner.

Takeaways

• 😀 The indirect fecal examination method is used to detect worm eggs in feces.
• 😀 The principle involves using a saturated NaCl solution, where worm eggs float on the surface of the liquid.
• 😀 Step 1: Fill a beaker with approximately 10 ml of saturated NaCl solution.
• 😀 Step 2: Add about half a teaspoon of fecal sample into the beaker.
• 😀 Step 3: Mix the fecal sample gently until the solution becomes homogeneous.
• 😀 Step 4: Prepare a clean glass slide and a test tube for the filtration process.
• 😀 Step 5: Pour the mixture into the test tube and filter through two layers of gauze to remove solid elements.
• 😀 Step 6: Allow the solution to stand for 45 minutes to 1 hour for proper separation.
• 😀 Step 7: After the settling period, prepare a microscope slide for observation.
• 😀 Step 8: Use a 1% or 2% iodine solution for staining the specimen on the slide.
• 😀 Step 9: Use tweezers to carefully place the specimen on the slide, ensuring no air bubbles are trapped.
• 😀 Step 10: The specimen is now ready for examination under a microscope.

Q & A

What is the purpose of the indirect feces examination method mentioned in the script?

The purpose of the indirect feces examination method is to detect parasitic eggs, specifically worm eggs, which float to the surface of a saturated NaCl solution when mixed with fecal matter.

What role does the saturated NaCl solution play in this examination process?

The saturated NaCl solution is used because it has a density that allows parasitic eggs, such as those of worms, to float when mixed with feces, aiding in their detection.

How long should the feces and NaCl solution mixture be left to stand?

The mixture should be left to stand for approximately 45 minutes to an hour to allow the parasitic eggs to float to the surface.

What is the first step in preparing the fecal specimen for examination?

The first step is to fill a chemical glass with about 10 ml of saturated NaCl solution, then add about half a teaspoon of the fecal specimen.

What should be done after adding the fecal specimen to the NaCl solution?

After adding the specimen, the mixture should be gently stirred to break up the fecal matter and create a homogeneous solution.

Why is filtering done with a two-layer gauze during the process?

The filtering with a two-layer gauze is done to remove any solid elements from the mixture, ensuring that only the liquid portion is used for further examination.

What is the purpose of using a glass slide and cover glass in this procedure?

A glass slide and cover glass are used to prepare the sample for microscopic examination, ensuring that the sample is properly prepared for staining and observation.

What staining solution is recommended for the microscopic examination?

A 1% or 2% Lugol's iodine solution is recommended for staining the specimen before it is examined under the microscope.

What should be checked before examining the prepared sample under a microscope?

Before examining the sample, it should be ensured that there are no air bubbles or excess water between the cover glass and the specimen.

What should the examiner look for during the microscopic examination?

The examiner should look for the presence of parasitic eggs, specifically those of worms, which will appear after proper staining.