Fouchet's test - for detection of bile pigments in urine

Summary

This video demonstrates the procedure for detecting bile pigments in urine using a simple test. It begins by preparing a clean test tube, adding 5 ml of urine, and a pinch of magnesium sulfate. Then, 1 ml of 10% barium chloride is added and the mixture is filtered. Afterward, a few drops of the fashion reagent are sprinkled onto the precipitate, resulting in a green color. This green hue confirms a positive result for bile pigments, specifically indicating the presence of bilirubin, which is crucial in diagnosing liver-related conditions.

Takeaways

• 😀 A test is being performed to detect bile pigments in urea.
• 😀 The necessary equipment for this test includes test tubes, a test tube stand, a measuring cylinder, a test holder, and a funnel.
• 😀 The test involves using magnesium sulfate (10%), barium chloride, and fashion reagent.
• 😀 First, a clean test tube is required to perform the test.
• 😀 5 ml of urine sample is added to the test tube.
• 😀 A pinch of magnesium sulfate is added to the test tube.
• 😀 1 ml of 10% barium chloride is added to the test tube.
• 😀 The contents are filtered after the reagents are added.
• 😀 The precipitate is collected after filtration.
• 😀 A few drops of fashion reagent are added to the precipitate, resulting in a formation of a green color.
• 😀 The appearance of the green color indicates a positive result for the fashion test.

Q & A

What is the purpose of the test described in the script?

The test is used to detect the presence of bile pigments in urine.

What equipment is required for this test?

The required equipment includes a test tube, test tube stand, measuring cylinder, test holder, and a funnel.

What chemicals are used in this test?

The chemicals used are magnesium sulfate (10%), barium chloride, and fashion reagent.

How is the urine sample prepared for the test?

A clean test tube is taken, and 5 ml of the urine sample is added to it.

What is the first step after adding the urine sample to the test tube?

A pinch of magnesium sulfate is added to the urine sample.

What happens after the magnesium sulfate is added?

1 ml of 10% barium chloride is added to the mixture, and the contents are then filtered.

What is done after filtering the contents?

The precipitate is taken, and a few drops of the fashion reagent are sprinkled onto it.

What indicates a positive result in this test?

The formation of a green color after adding the fashion reagent indicates a positive result.

Why is the green color significant?

The green color signifies the presence of bile pigments in the urine sample, confirming a positive fashion test.

What does the test specifically detect in the urine?

The test specifically detects bile pigments, which can be indicative of liver or biliary system conditions.