Pengambilan Sample Uji Air Limbah

Summary

This video outlines the procedure for collecting a wastewater sample in a sterile and aseptic manner. It demonstrates the necessary tools and steps, such as sterilizing the sample bottle with a Bunsen burner, carefully filling the bottle with a specified volume of water, and properly sealing and labeling the sample. The process emphasizes safety and precision, ensuring accurate and uncontaminated sample collection. The video serves as a practical guide for individuals needing to collect and store water samples for testing or analysis.

Takeaways

• 😀 Prepare necessary equipment including sterilized sample bottles, string, alcohol spray, gloves, Bunsen burner, and lighter or matches.
• 😀 Follow aseptic procedures during the sample collection to ensure no contamination of the sample.
• 😀 Tie the sample bottle securely with string to prevent accidental spills or contamination.
• 🔥 Use a Bunsen burner to sterilize the mouth and neck of the sample bottle before opening it.
• 💧 Immerse the sample bottle slowly into the water surface to collect the sample, ensuring a minimum volume of 100 ml (± 2.5 ml).
• 🔥 After collecting the sample, sterilize the bottle’s mouth and neck again with the Bunsen burner before closing it.
• 📝 Record important information on a label to identify the sample, such as the time and location of collection.
• 🏷️ Attach the label securely to the sample bottle to ensure proper identification and tracking.
• 📦 Place the filled sample bottle along with any used equipment back into the sample box for storage or transport.
• 🧴 Make sure to use alcohol spray (70%) for disinfecting equipment and your hands before and after handling the sample bottle.

Q & A

What equipment is required for the wastewater sampling procedure?

The required equipment includes a sterile sample bottle, string, 70% alcohol spray, gloves, a Bunsen burner, a lighter or match, and other necessary tools for the procedure.

Why is it important to handle the sample aseptically?

Aseptically handling the sample is crucial to avoid contamination, ensuring the integrity of the sample for accurate testing and analysis.

What is the purpose of tying the bottle neck with string during the sampling process?

Tying the bottle neck with string helps secure the bottle and makes it easier to handle during the sample collection process.

How is the Bunsen burner used in the sampling process?

The Bunsen burner is used to sterilize the mouth and neck of the sample bottle before and after filling it with the wastewater sample.

What should be done to the bottle before it is filled with the sample?

Before filling the bottle, the mouth and neck should be sterilized by burning them over the flame of the Bunsen burner to prevent contamination.

How much wastewater should be collected in the sample bottle?

The sample bottle should be filled with at least 100 ml of wastewater, with a tolerance of ±2.5 ml.

Why is it necessary to burn the bottle's mouth and neck after it is filled?

Burning the bottle's mouth and neck after filling it ensures any potential contamination from the environment is eliminated before sealing the bottle.

What information should be recorded on the sample label?

The label should include relevant details about the sample, such as the date, location, and any other necessary information that helps in identifying the sample.

Where should the label be placed on the sample bottle?

The label should be securely attached to the body of the sample bottle for easy identification.

What should be done with the collected sample and equipment after the process is completed?

After collecting the sample, place the sample bottle along with the used equipment back into a sample box for storage and further analysis.