ACARA 13 REPRODUKSI : ANALISIS KUALITAS SPERMA (Praktikum Fisiologi Hewan 2020)

Summary

This video presents a practical session on animal physiology, focusing on sperm quality analysis. It details the procedure for creating a spermatozoa suspension from the male Mus musculus, including dissection techniques and the extraction of cauda epididymis. The video also covers methods for assessing sperm viability and morphology using methylene blue staining, alongside observations of sperm motility through microscopy. With clear instructions and visuals, the session aims to educate viewers on the critical aspects of reproductive physiology in animals.

Takeaways

• 😀 The practical session focuses on animal physiology, specifically reproduction and sperm quality analysis.
• 😀 The first step involves dislocating the male mouse (Mus musculus) for dissection.
• 😀 The cauda epididymis is extracted during the dissection for further analysis.
• 😀 A physiological saline solution is used to create a spermatozoa suspension.
• 😀 Viability and morphology of spermatozoa are assessed using methylene blue staining.
• 😀 Live spermatozoa appear transparent, while dead ones absorb the methylene blue dye.
• 😀 The percentage of live and abnormal sperm cells is calculated during the examination.
• 😀 The motility of spermatozoa is analyzed using a microscope.
• 😀 Different types of sperm movement are observed, including spinning and stationary behavior.
• 😀 The practical concludes with a summary of the findings and appreciation for participation.

Q & A

What is the primary focus of the practical session discussed in the video?

The primary focus is on animal physiology, specifically on the reproduction and analysis of sperm quality.

What is the first step in creating a spermatozoa suspension?

The first step is to dislocate the cervical region of the male Mus musculus by pinching its head and pulling its tail in a single swift motion.

How is the cauda epididymis obtained during the dissection?

The Mus musculus is dissected ventrally to retrieve the cauda epididymis.

What is done with the cauda epididymis after it is cut?

It is placed in a petri dish containing 2-3 milliliters of warm physiological saline for further processing.

How is the spermatozoa suspension prepared?

The tissue is chopped or diced to create a suspension of spermatozoa.

What method is used to assess sperm viability and morphology?

Suspensions of spermatozoa are placed on a slide, mixed with 1-2 drops of methylene blue, and then observed under a microscope.

What indicates that a sperm cell is alive when stained with methylene blue?

Live sperm cells appear transparent, while dead cells absorb the blue dye.

What additional analysis is performed on the spermatozoa?

The percentage of abnormal sperm shapes is calculated, in addition to the percentage of live cells.

What is evaluated regarding the movement of spermatozoa?

The spermatozoa's movement patterns are observed under a microscope to assess their motility.

What types of movement were noted during the practical observation?

Different movement patterns were observed, including sperm moving in place and those exhibiting rotational movement.